Tc8, a Tourist-like Transposon in Caenorhabditis elegans

Genetics ◽  
2001 ◽  
Vol 158 (3) ◽  
pp. 1081-1088 ◽  
Author(s):  
Quang Hien Le ◽  
Kime Turcotte ◽  
Thomas Bureau
Keyword(s):  
Caenorhabditis Elegans ◽  
Expressed Sequence Tags ◽  
Large Family ◽  
Insertion Sequences ◽  
Normal Plant ◽  
Nematode Caenorhabditis Elegans ◽  
Plant Genomes ◽  
Plant Genes ◽  
Expressed Sequence ◽  
Eukaryotic Genomes

Abstract Members of the Tourist family of miniature inverted-repeat transposable elements (MITEs) are very abundant among a wide variety of plants, are frequently found associated with normal plant genes, and thus are thought to be important players in the organization and evolution of plant genomes. In Arabidopsis, the recent discovery of a Tourist member harboring a putative transposase has shed new light on the mobility and evolution of MITEs. Here, we analyze a family of Tourist transposons endogenous to the genome of the nematode Caenorhabditis elegans (Bristol N2). One member of this large family is 7568 bp in length, harbors an ORF similar to the putative Tourist transposase from Arabidopsis, and is related to the IS5 family of bacterial insertion sequences (IS). Using database searches, we found expressed sequence tags (ESTs) similar to the putative Tourist transposases in plants, insects, and vertebrates. Taken together, our data suggest that Tourist-like and IS5-like transposons form a superfamily of potentially active elements ubiquitous to prokaryotic and eukaryotic genomes.

scholarly journals Analysis of expressed sequence tags derived from the endophytic fungus Neotyphodium lolii grown in vitro and in association with its host plant perennial ryegrass

2007 ◽  
Vol 13 ◽  
pp. 501-504
Author(s):  
R. Johnson ◽  
A. Khan ◽  
C. Voisey ◽  
S. Bassett ◽  
C. Gaborit ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Virulence Genes ◽  
In Planta ◽  
Suppression Subtractive Hybridisation ◽  
The Public ◽  
Neotyphodium Lolii ◽  
Subtractive Hybridisation ◽  
Plant Genes ◽  
Expressed Sequence

As a first step towards a functional genomics approach to gain a greater understanding of this important symbiosis, we have generated, sequenced and analysed two EST libraries from cultures of N. lolii and six in planta subtracted EST libraries enriched for differentially expressed genes. A total of 12871 ESTs were sequenced which, after filtering for quality, clustered into 1066 contigs and 3230 singletons to give a set of 4296 unique sequences or unigenes. BLASTX analysis revealed that 60% of fungal sequences derived from cultures were of unknown function with a sub-set of these corresponding to orphans. For the in planta-derived ESTs, most of the sequences with homologs in the public databases (98%) were of ryegrass origin. Comparisons made against fully sequenced genomes revealed that most fungal ESTs were homologous to genes present in both pathogenic and non-pathogenic ascomycete filamentous fungi, whereas the subtracted libraries comprised mostly plant genes. A range of sequences having significant homology to demonstrated pathogenicity/virulence genes in other fungal pathosystems were also identified, as well as some ESTs with proven roles in endophyte secondary metabolism. Keywords: ESTs, cDNA, Neotyphodium lolii, Lolium perenne, symbiosis, mutualism, suppression subtractive hybridisation

Caenorhabditis elegans expressed sequence tags identify gene families and potential disease gene homologues

1992 ◽  
Vol 1 (2) ◽  
pp. 124-131 ◽  
Author(s):  
W. Richard McCombie ◽  
Mark D. Adams ◽  
Jenny M. Kelley ◽  
Michael G. FitzGerald ◽  
Teresa R. Utterback ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Expressed Sequence Tags ◽  
Disease Gene ◽  
Gene Families ◽  
Expressed Sequence

Plant transposable elements: Their role in evolution

1988 ◽  
Vol 8 (6) ◽  
pp. 585-588 ◽  
Author(s):  
Heinz Saedler
Keyword(s):  
Transposable Elements ◽  
Biological Significance ◽  
Normal Plant ◽  
Wild Type ◽  
Induced Mutations ◽  
Plant Genomes ◽  
Plant Genes ◽  
Structure And Functions

Transposable elements (TE) are natural constituents of plant genomes. However, their presence only becomes apparent if they become dislodged from their resident positions in the genome and transpore into another gene, thereby inducing a mutation. Such TE-induced mutations are somatically unstable because they revert to wild type and hence reconstitute the expression of the mutated gene. The frequent somatic excision of the TE results in a variegated phenotype. Since this instability is inherited in a Mendelian manner the variegated phenotype is nuclear determined. By this criterion TE have been shown to occur in more than 30 species belonging to different families and genera. Many questions arise when dealing with TE: their structure and functions, and the biological significance of the activity of elements in the differentiation of a normal plant or in the evolution of plant genes.

scholarly journals Expressed Sequence Tags from Roots and Nodule Primordia of Lotus japonicus Infected with Mesorhizobium loti

2002 ◽  
Vol 15 (4) ◽  
pp. 376-379 ◽  
Author(s):  
Carsten Poulsen ◽  
Lone Pødenphant
Keyword(s):  
Expressed Sequence Tags ◽  
Messenger Rna ◽  
Root Nodules ◽  
Lotus Japonicus ◽  
Cdna Expression Library ◽  
Expression Library ◽  
Mesorhizobium Loti ◽  
Pcr Products ◽  
Plant Genes ◽  
Expressed Sequence

Messenger RNA from young Lotus japonicus roots carrying root nodule primordia appearing after inoculation with Mesorhizobium loti bacteria were used to construct a cDNA expression library. Single-pass sequencing employing colony-polymerase chain reaction (PCR) and analysis of PCR products established a total of 2,397 new expressed sequence tags (ESTs). We have putatively identified 1,236 known and 484 hypothetical proteins coded by the corresponding mRNAs. The remaining cDNAs are unknown (316) or redundant overlapping cDNAs (361). We hope that this batch of ESTs will assist in the recognition of plant genes involved during development of nitrogen-fixing root nodules.

Expressed sequence tags from cold-acclimatized barley can identify novel plant genes

2001 ◽  
Vol 120 (6) ◽  
pp. 497-502 ◽  
Author(s):  
P. FACCIOLI ◽  
N. PECCHIONI ◽  
L. CATTIVELLI ◽  
A. M. STANCA ◽  
V. TERZI
Keyword(s):  
Expressed Sequence Tags ◽  
Plant Genes ◽  
Expressed Sequence

scholarly journals The sugarcane signal transduction (SUCAST) catalogue: prospecting signal transduction in sugarcane

2001 ◽  
Vol 24 (1-4) ◽  
pp. 25-34 ◽  
Author(s):  
Glaucia Mendes Souza ◽  
Ana Carolina Quirino Simoes ◽  
Katia Cristina Oliveira ◽  
Humberto Miguel Garay ◽  
Leonardo Costa Fiorini ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Expressed Sequence Tags ◽  
Tissue Specificity ◽  
Expressed Sequence Tag ◽  
Large Family ◽  
Small Gtpases ◽  
Response Regulators ◽  
New Genes ◽  
Est Sequencing ◽  
Expressed Sequence

EST sequencing has enabled the discovery of many new genes in a vast array of organisms, and the utility of this approach to the scientific community is greatly increased by the establishment of fully annotated databases. The present study aimed to identify sugarcane ESTs sequenced in the sugarcane expressed sequence tag (SUCEST) project (<A HREF="http://sucest.lad.ic.unicamp.br/">http://sucest.lad.ic.unicamp.br</A>) that corresponded to signal transduction components. We also produced a sugarcane signal transduction (SUCAST) catalogue (<A HREF="http://sucest.lad.ic.unicamp.br/private/mining-reports/QG/QG-mining.htm">http://sucest.lad.ic.unicamp.br/private/mining-reports/QG/QG-mining.htm</A>) that covered the main categories and pathways. Expressed sequence tags (ESTs) encoding enzymes for hormone (gibberellins, ethylene, auxins, abscisic acid and jasmonic acid) biosynthetic pathways were found and tissue specificity was inferred from their relative frequency of occurrence in the different libraries. Whenever possible, transducers of hormones and plant peptide signaling were catalogued to the respective pathway. Over 100 receptors were found in sugarcane, which contains a large family of Ser/Thr kinase receptors and also photoreceptors, histidine kinase receptors and their response regulators. G-protein and small GTPases were analyzed and compared to known members of these families found in mammalian and plant systems. Major kinase and phosphatase pathways were mapped, with special attention being given to the MAP kinase and the inositol pathway, both of which are well known in plants.

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