scholarly journals A Genetic Linkage Map of the Model Legume Lotus japonicus and Strategies for Fast Mapping of New Loci

Genetics ◽  
2002 ◽  
Vol 161 (4) ◽  
pp. 1673-1683 ◽  
Author(s):  
Niels Sandal ◽  
Lene Krusell ◽  
Simona Radutoiu ◽  
Magdalena Olbryt ◽  
Andrea Pedrosa ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Map ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Lotus Japonicus ◽  
Aflp Markers ◽  
Linkage Groups ◽  
Model Legume ◽  
Group I

Abstract A genetic map for the model legume Lotus japonicus has been developed. The F2 mapping population was established from an interspecific cross between L. japonicus and L. filicaulis. A high level of DNA polymorphism between these parents was the source of markers for linkage analysis and the map is based on a framework of amplified fragment length polymorphism (AFLP) markers. Additional markers were generated by restriction fragment length polymorphism (RFLP) and sequence-specific PCR. A total of 524 AFLP markers, 3 RAPD markers, 39 gene-specific markers, 33 microsatellite markers, and six recessive symbiotic mutant loci were mapped. This genetic map consists of six linkage groups corresponding to the six chromosomes in L. japonicus. Fluorescent in situ hybridization (FISH) with selected markers aligned the linkage groups to chromosomes as described in the accompanying article by Pedrosa  et al. (2002, this issue). The length of the linkage map is 367 cM and the average marker distance is 0.6 cM. Distorted segregation of markers was found in certain sections of the map and linkage group I could be assembled only by combining colormapping and cytogenetics (FISH). A fast method to position genetic loci employing three AFLP primer combinations yielding 89 markers was developed and evaluated by mapping three symbiotic loci, Ljsym1, Ljsym5, and Ljhar1-3.

An Amplified Fragment Length Polymorphism Map of the Silkworm

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1277-1284 ◽  
Author(s):  
Yuan-De Tan ◽  
Chunling Wan ◽  
Yufang Zhu ◽  
Chen Lu ◽  
Zhonghuai Xiang ◽  
...  
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Polymorphic Markers ◽  
Linkage Groups ◽  
Group 3

Abstract The silkworm (Bombyx mori L.) is a lepidopteran insect with a long history of significant agricultural value. We have constructed the first amplified fragment length polymorphism (AFLP) genetic linkage map of the silkworm B. mori at a LOD score of 2.5. The mapping AFLP markers were genotyped in 47 progeny from a backcross population of the cross no. 782 × od100. A total of 1248 (60.7%) polymorphic AFLP markers were detected with 35 PstI/TaqI primer combinations. Each of the primer combinations generated an average of 35.7 polymorphic AFLP markers. A total of 545 (44%) polymorphic markers are consistent with the expected segregation ratio of 1:1 at the significance level of P = 0.05. Of the 545 polymorphic markers, 356 were assigned to 30 linkage groups. The number of markers on linkage groups ranged from 4 to 36. There were 21 major linkage groups with 7-36 markers and 9 relatively small linkage groups with 4-6 markers. The 30 linkage groups varied in length from 37.4 to 691.0 cM. The total length of this AFLP linkage map was 6512 cM. Genetic distances between two neighboring markers on the same linkage group ranged from 0.2 to 47 cM with an average of 18.2 cM. The sex-linked gene od was located between the markers P1T3B40 and P3T3B27 at the end of group 3, indicating that AFLP linkage group 3 was the Z (sex) chromosome. This work provides an essential basic map for constructing a denser linkage map and for mapping genes underlying agronomically important traits in the silkworm B. mori L.

scholarly journals A Molecular Genetic Map and Electrophoretic Karyotype of the Plant Pathogenic Fungus Cochliobolus sativus

2002 ◽  
Vol 15 (5) ◽  
pp. 481-492 ◽  
Author(s):  
Shaobin Zhong ◽  
Brian J. Steffenson ◽  
J. Patrick Martinez ◽  
Lynda M. Ciuffetti
Keyword(s):  
Genetic Map ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Molecular Genetic ◽  
Aflp Markers ◽  
Cochliobolus Sativus ◽  
Electrophoretic Karyotype ◽  
Linkage Groups ◽  
Molecular Genetic Map ◽  
Map Distance

A molecular genetic map was constructed and an electrophoretic karyotype was resolved for Cochliobolus sativus, the causal agent of spot blotch of barley and wheat. The genetic map consists of 27 linkage groups with 97 amplified fragment length polymorphism (AFLP) markers, 31 restriction fragment length polymorphism (RFLP) markers, two polymerase chain reaction amplified markers, the mating type locus (CsMAT), and a gene (VHv1) conditioning high virulence on barley cv. Bowman. These linkage groups covered a map distance of 849 cM. The virulence gene VHv1 cosegregated with six AFLP markers and was mapped on one of the major linkage groups. Fifteen chromosome-sized DNAs were resolved in C. sativus isolates ND93-1 and ND90Pr with contour-clamped homogeneous electric field (CHEF) electrophoresis combined with telo-mere probe analysis of comigrating chromosome-sized DNAs. The chromosome sizes ranged from 1.25 to 3.80 Mbp, and the genome size of the fungus was estimated to be approximately 33 Mbp. By hybridizing genetically mapped RFLP and AFLP markers to CHEF blots, 25 of the 27 linkage groups were assigned to specific chromosomes. The barley-specific virulence locus VHv1 was localized on a chromosome of 2.80 Mbp from isolate ND90Pr in the CHEF gel. The total map length of the fungus was estimated to be at least 1,329 cM based on the map distance covered by the linked markers and the estimated gaps. Therefore, the physical to genetic distance ratio is approximately 25 kb/cM. Construction of a high-resolution map around target loci will facilitate the cloning of the genes conferring virulence and other characters in C. sativus by a map-based cloning strategy.

Genetic map of triticale compiling DArT, SSR, and AFLP markers

Genome ◽  
2011 ◽  
Vol 54 (5) ◽  
pp. 391-401 ◽  
Author(s):  
M. Tyrka ◽  
P.T. Bednarek ◽  
A. Kilian ◽  
M. Wędzony ◽  
T. Hura ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Map ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Structural Rearrangements ◽  
Linkage Groups ◽  
Array Technology ◽  
Dh Lines ◽  
Simple Sequence

A set of 90 doubled haploid (DH) lines derived from F1plants that originated from a cross between × Triticosecale Wittm. ‘Saka3006’ and ×Triticosecale Wittm. ‘Modus’, via wide crossing with maize, were used to create a genetic linkage map of triticale. The map has 21 linkage groups assigned to the A, B, and R genomes including 155 simple sequence repeat (SSR), 1385 diversity array technology (DArT), and 28 amplified fragment length polymorphism (AFLP) markers covering 2397 cM with a mean distance between two markers of 4.1 cM. Comparative analysis with wheat consensus maps revealed that triticale chromosomes of the A and B genomes were represented by 15 chromosomes, including combinations of 2AS.2AL#, 2AL#2BL, 6AS.6AL#, and 2BS.6AL# instead of 2A, 2B, and 6A. In respect to published maps of rye, substantial rearrangements were found also for chromosomes 1R, 2R, and 3R of the rye genome. Chromosomes 1R and 2R were truncated and the latter was linked with 3R. A nonhomogeneous distribution of markers across the triticale genome was observed with evident bias (48%) towards the rye genome. This genetic map may serve as a reference linkage map of triticale for efficient studies of structural rearrangements, gene mapping, and marker-assisted selection.

Efficiency of RFLP, RAPD, and AFLP markers for the construction of an intraspecific map of the tomato genome

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Vera Saliba-Colombani ◽  
Mathilde Causse ◽  
Laurent Gervais ◽  
Jacqueline Philouze
Keyword(s):  
Lycopersicon Esculentum ◽  
Linkage Map ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Aflp Markers ◽  
Rflp Rapd

We have constructed a tomato genetic linkage map based on an intraspecific cross between two inbred lines of Lycopersicon esculentum and L. esculentum var. cerasiforme. The segregating population was composed of 153 recombinant inbred lines. This map is comprised of one morphological, 132 RFLP (restriction fragment length polymorphism, including 16 known-function genes), 33 RAPD (random amplified polymorphic DNA), and 211 AFLP (amplified fragment length polymorphism) loci. We compared the 3 types of markers for their polymorphism, segregation, and distribution over the genome. RFLP, RAPD, and AFLP methods revealed 8.7%, 15.8%, and 14.5% informative bands, respectively. This corresponded to polymorphism in 30% of RFLP probes, 32% of RAPD primers, and 100% of AFLP primer combinations. Less deviation from the 1:1 expected ratio was obtained with RFLP than with AFLP loci (8% and 18%, respectively). RAPD and AFLP markers were not randomly distributed over the genome. Most of them (60% and 80%, respectively) were grouped in clusters located around putative centromeric regions. This intraspecific map spans 965 cM with an average distance of 8.3 cM between markers (of the framework map). It was compared to other published interspecific maps of tomato. Despite the intraspecific origin of this map, it did not show any increase in length when compared to the high-density interspecific map of tomato. Key words: Lycopersicon esculentum, molecular linkage map, RFLP, AFLP, intraspecific cross.

An integrated restriction fragment length polymorphism - amplified fragment length polymorphism linkage map for cultivated sunflower

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Melaku Ayele Gedil ◽  
Crispin Wye ◽  
Simon Berry ◽  
Bart Segers ◽  
Johan Peleman ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Restriction Fragment Length

Restriction fragment length polymorphism (RFLP) maps have been constructed for cultivated sunflower (Helianthus annuus L.) using three independent sets of RFLP probes. The aim of this research was to integrate RFLP markers from two sets with RFLP markers for resistance gene candidate (RGC) and amplified fragment length polymorphism (AFLP) markers. Genomic DNA samples of HA370 and HA372, the parents of the F2 population used to build the map, were screened for AFLPs using 42 primer combinations and RFLPs using 136 cDNA probes (RFLP analyses were performed on DNA digested with EcoRI, HindIII, EcoRV, or DraI). The AFLP primers produced 446 polymorphic and 1101 monomorphic bands between HA370 and HA372. The integrated map was built by genotyping 296 AFLP and 104 RFLP markers on 180 HA370 × HA372 F2 progeny (the AFLP marker assays were performed using 18 primer combinations). The HA370 × HA372 map comprised 17 linkage groups, presumably corresponding to the 17 haploid chromosomes of sunflower, had a mean density of 3.3 cM, and was 1326 cM long. Six RGC RFLP loci were polymorphic and mapped to three linkage groups (LG8, LG13, and LG15). AFLP markers were densely clustered on several linkage groups, and presumably reside in centromeric regions where recombination is reduced and the ratio of genetic to physical distance is low. Strategies for targeting markers to euchromatic DNA need to be tested in sunflower. The HA370 × HA372 map integrated 14 of 17 linkage groups from two independent RFLP maps. Three linkage groups were devoid of RFLP markers from one of the two maps.Key words: amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), Helianthus, sunflower, genetic map.

Applications of genetic markers in cytogenetic manipulation of the wheat genomes

Genome ◽  
1989 ◽  
Vol 31 (1) ◽  
pp. 137-142 ◽  
Author(s):  
M. D. Gale ◽  
P. J. Sharp ◽  
S. Chao ◽  
C. N. Law
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Genetic Maps ◽  
Linkage Groups ◽  
Large Genome ◽  
Large Genome Size ◽  
Restriction Fragment Length

A molecular map of wheat, Triticum aestivum, is being developed. Problems associated with the large genome size, the large number of linkage groups, polyploidy, and limited polymorphism at the DNA level are being overcome. In addition to the breeding applications expected from the map, various uses for restriction fragment length polymorphism markers as tools in cytogenetic manipulation of wheat chromosomes and those from related species are being found. These include identification of aneuploid genotypes, added precision in intervarietal chromosome manipulations, tests of chromosome stability, identification of alien chromosomes, and marker-aided introgression of genes of agronomic importance from related species.Key words: wheat, restriction fragment length polymorphism, genetic maps, aneuploidy, alien chromosomes.

Sign in / Sign up

Export Citation Format

Share Document