An AFLP-Based Genetic Linkage Map of Channel Catfish (Ictalurus punctatus) Constructed by Using an Interspecific Hybrid Resource Family

Genetics ◽  
2003 ◽  
Vol 165 (2) ◽  
pp. 687-694 ◽  
Author(s):  
Zhanjiang Liu ◽  
Attila Karsi ◽  
Ping Li ◽  
Dongfeng Cao ◽  
R Dunham
Keyword(s):  
Linkage Map ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
The United States ◽  
Interspecific Backcross ◽  
Aflp Markers ◽  
Production Traits ◽  
Blue Catfish

Abstract Catfish is the major aquaculture species in the United States. The hybrid catfish produced by crossing channel catfish females with blue catfish males exhibit a number of desirable production traits, but their mass production has been difficult. To introduce desirable genes from blue catfish into channel catfish through introgression, a genetic linkage map is helpful. In this project, a genetic linkage map was constructed using amplified fragment length polymorphism (AFLP). A total of 607 AFLP markers were analyzed using 65 primer combinations and an interspecific backcross resource family. A total of 418 AFLP markers were assigned to 44 linkage groups. Among the remaining 189 markers, 101 were not used because of significant segregation distortion, 29 were unlinked, and 59 were eliminated because they span very large distances. The 418 AFLP markers covered 1593 cM Kosambi. The AFLP markers showed a high level of clustering that appears to be related to certain primer combinations. This linkage map will serve as the basis for mapping a greater number of markers to provide a map with high enough resolution for it to be useful for selective breeding programs using introgression.

A Microsatellite-Based Genetic Linkage Map for Channel Catfish, Ictalurus punctatus

Genetics ◽  
2001 ◽  
Vol 158 (2) ◽  
pp. 727-734 ◽  
Author(s):  
Geoffrey C Waldbieser ◽  
Brian G Bosworth ◽  
Danny J Nonneman ◽  
William R Wolters
Keyword(s):  
Linkage Map ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Microsatellite Loci ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Type I ◽  
Type Ii ◽  
Linkage Groups ◽  
Multipoint Linkage

Abstract Microsatellite loci were identified in channel catfish gene sequences or random clones from a small insert genomic DNA library. Outbred populations of channel catfish contained an average of eight alleles per locus and an average heterozygosity of 0.70. A genetic linkage map of the channel catfish genome (N = 29) was constructed from two reference families. A total of 293 microsatellite loci were polymorphic in one or both families, with an average of 171 informative meioses per locus. Nineteen type I loci, 243 type II loci, and one EST were placed in 32 multipoint linkage groups covering 1958 cM. Nine more type II loci were contained in three two-point linkage groups covering 24.5 cM. Twenty-two type II loci remained unlinked. Multipoint linkage groups ranged in size from 11.9 to 110.5 cM with an average intermarker distance of 8.7 cM. Seven microsatellite loci were closely linked with the sex-determining locus. The microsatellite loci and genetic linkage map will increase the efficiency of selective breeding programs for channel catfish.

A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and the localization of fusarium vascular wilt resistance

2005 ◽  
Vol 110 (4) ◽  
pp. 669-677 ◽  
Author(s):  
A. Hamwieh ◽  
S. M. Udupa ◽  
W. Choumane ◽  
A. Sarker ◽  
F. Dreyer ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Aflp Markers ◽  
Vascular Wilt ◽  
Wilt Resistance

A first genetic linkage map of bluegill sunfish (Lepomis macrochirus) using AFLP markers

2009 ◽  
Vol 18 (5) ◽  
pp. 825-835 ◽  
Author(s):  
Wei-Ji Wang ◽  
Han-Ping Wang ◽  
Hong Yao ◽  
Geoff K. Wallat ◽  
Laura G. Tiu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Lepomis Macrochirus ◽  
Aflp Markers ◽  
Bluegill Sunfish

CONSTRUCTION OF A MOLECULAR GENETIC LINKAGE MAP FOR LONGAN BASED ON RAPD, ISSR, SRAP AND AFLP MARKERS

2010 ◽  
pp. 141-148 ◽  
Author(s):  
Y.S. Guo ◽  
C.J. Liu ◽  
Y.H. Zhao ◽  
J.H. Xie ◽  
P.R. Ren ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Molecular Genetic ◽  
Aflp Markers

Construction of a genetic linkage map and identification of AFLP markers for resistance to root-knot nematodes in peach rootstocks

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Zhen-Xiang Lu ◽  
B Sosinski ◽  
G L Reighard ◽  
W V Baird ◽  
A G Abbott
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Prunus Persica ◽  
Aflp Marker ◽  
Aflp Markers ◽  
Single Linkage ◽  
Root Knot Nematodes ◽  
Group I ◽  
Peach Rootstocks

A genetic linkage map for peach (Prunus persica (L.) Batsch) rootstocks has been constructed using amplified fragment length polymorphism (AFLP) markers in 55 F2 individuals derived from the cross Lovell x Nemared. From 21 different primer combinations, a total of 169 AFLP markers were scored, of which, 153 were assigned to 15 linkage groups covering 1297 centimorgans (cM) of the peach nuclear genome. The average interval between these markers was 9.1 cM. Two genes (Mi and Mij) involved in resistance to root-knot nematodes (Meloidogyne incognita (Kofoid and White) Chitwood and Meloidogyne javanica (Treub) Chitwood) were mapped to a single linkage group (Group I). These two loci were separated by a 16.5-cM interval. One codominant AFLP marker (EAA/MCAT10) was tightly linked to the Mij locus (3.4 cM), and a dominant AFLP marker (EAT/MCAT2) was found to be closely associated with the Mi locus (6.0 cM). These markers are being studied for utilization in peach rootstock breeding with marker-assisted selection.Key words: peach rootstocks, root-knot nematodes, resistance, AFLP, mapping.

scholarly journals A genetic linkage map of mouse chromosome 10: localization of eighteen molecular markers using a single interspecific backcross.

Genetics ◽  
1990 ◽  
Vol 125 (4) ◽  
pp. 855-866
Author(s):  
M J Justice ◽  
L D Siracusa ◽  
D J Gilbert ◽  
N Heisterkamp ◽  
J Groffen ◽  
...  
Keyword(s):  
Linkage Map ◽  
Mouse Chromosome ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Molecular Genetic ◽  
Interspecific Backcross ◽  
Mus Spretus ◽  
Chromosome 10 ◽  
Genetic Length ◽  
First Time

Abstract Interspecific mouse backcross analysis was used to generate a molecular genetic linkage map of mouse chromosome 10. The map locations of the Act-2, Ahi-1, Bcr, Braf, Cdc-2a, Col6a-1, Col6a-2, Cos-1, Esr, Fyn, Gli, Ifg, Igf-1, Myb, Pah, pgcha, Ros-1 and S100b loci were determined. These loci extend over 80% of the genetic length of the chromosome, providing molecular access to many regions of chromosome 10 for the first time. The locations of the genes mapped in this study extend the known regions of synteny between mouse chromosome 10 and human chromosomes 6, 10, 12 and 21, and reveal a novel homology segment between mouse chromosome 10 and human chromosome 22. Several loci may lie close to, or correspond to, known mutations. Preferential transmission of Mus spretus-derived alleles was observed for loci mapping to the central region of mouse chromosome 10.

A genetic linkage map of swimming crab (Portunus trituberculatus) based on SSR and AFLP markers

Aquaculture ◽  
2012 ◽  
Vol 344-349 ◽  
pp. 66-81 ◽  
Author(s):  
Lei Liu ◽  
Jian Li ◽  
Ping Liu ◽  
Fazhen Zhao ◽  
Baoquan Gao ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Aflp Markers ◽  
Portunus Trituberculatus ◽  
Swimming Crab
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