Use of a Direct Fluorescent Antibody Test for Detecting Chlamydia trachomatis Cervical Infection in Women Seeking Routine Gynecologic Care

1987 ◽  
Vol 156 (4) ◽  
pp. 575-581 ◽  
Author(s):  
R. S. Phillips ◽  
P. A. Hanff ◽  
R. S. Kauffman ◽  
M. D. Aronson
Keyword(s):  
Chlamydia Trachomatis ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Cervical Infection ◽  
Direct Fluorescent Antibody

Confirmation of the Syva MicroTrak Enzyme Immunoassay for Chlamydia trachomatis by Syva Direct Fluorescent Antibody Test

1996 ◽  
Vol 23 (6) ◽  
pp. 465-470 ◽  
Author(s):  
JAMES L. BEEBE ◽  
HELEN MASTERS ◽  
DONALD JUNGKIND ◽  
DAVID M. HELTZEL ◽  
ADRIANA WEINBERG
Keyword(s):  
Chlamydia Trachomatis ◽  
Enzyme Immunoassay ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Direct Fluorescent Antibody

scholarly journals A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes

2017 ◽  
Vol 9 (01) ◽  
pp. 053-056 ◽  
Author(s):  
Vrushali Patwardhan ◽  
Preena Bhalla ◽  
Deepti Rawat ◽  
Vijay Kumar Garg ◽  
Kabir Sardana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Genital Ulcer ◽  
Chain Reaction ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Simplex Virus

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

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