Comparison of direct, Rapid Immunohistochemical Test Performance with Direct Fluorescent Antibody Test for rabies diagnosis in Ethiopia

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

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Comparative evaluation of direct fluorescent antibody test, Seller's staining test and rapid immunodiagnostic assay for post-mortem diagnosis of rabies in cattle

Indian Journal of Veterinary Pathology ◽

10.5958/0973-970x.2019.00034.8 ◽

2019 ◽

Vol 43 (3) ◽

pp. 165

Author(s):

Monika Thakur ◽

B.S. Sandhu

Keyword(s):

Comparative Evaluation ◽

Fluorescent Antibody ◽

Antibody Test ◽

Post Mortem ◽

Direct Fluorescent Antibody

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Salmonid whirling disease: myxosporean and actinosporean stages cross-react in direct fluorescent antibody test

Journal of Fish Diseases ◽

10.1111/j.1365-2761.1989.tb00285.x ◽

1989 ◽

Vol 12 (2) ◽

pp. 137-141 ◽

Cited By ~ 21

Author(s):

M. E. MARKIW

Keyword(s):

Fluorescent Antibody ◽

Antibody Test ◽

Whirling Disease ◽

Direct Fluorescent Antibody

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Comparison of Automated Quantitative Reverse Transcription-PCR and Direct Fluorescent-Antibody Detection for Routine Rabies Diagnosis in the United States

Journal of Clinical Microbiology ◽

10.1128/jcm.01227-15 ◽

2015 ◽

Vol 53 (9) ◽

pp. 2983-2989 ◽

Cited By ~ 15

Author(s):

Michelle Dupuis ◽

Scott Brunt ◽

Kim Appler ◽

April Davis ◽

Robert Rudd

Keyword(s):

United States ◽

Reverse Transcription ◽

Gold Standard ◽

Fluorescent Antibody ◽

The United States ◽

Pcr Assay ◽

Qrt Pcr ◽

Reverse Transcription Pcr ◽

Direct Fluorescent Antibody ◽

Rabies Diagnosis

Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunction with quantitative reverse transcription-PCR (qRT-PCR), to augment or potentially replace the DFA test, was the focus of this project. Here we describe a triplex qRT-PCR assay, including assembly and evaluation for sensitivity, specificity, and ability to detect variants. Additionally, we compared the qRT-PCR assay to the gold standard direct fluorescent-antibody test. More than 1,000 specimens submitted for routine rabies diagnosis were tested to directly compare the two methods. All results were in agreement between the two methods, with one additional specimen detected by qRT-PCR below the limits of the DFA sensitivity. With the proper continued validation for variant detection, molecular methods have a place in routine rabies diagnostics within the United States.

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