The use of immunochemically purified anti-brucella antibody in a direct fluorescent antibody test for Brucella abortus

1980 ◽  
Vol 1 (4) ◽  
pp. 343-352 ◽  
Author(s):  
Keith W. Thomas ◽  
Ian P. McCausland
Keyword(s):  
Brucella Abortus ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Direct Fluorescent Antibody

scholarly journals Direct fluorescent antibody test and bacteriological culture for detection of Brucella suis in swine tissues

2019 ◽  
Vol 30 (1) ◽  
pp. 39
Author(s):  
S M. Estein ◽  
A R. Bence ◽  
C S. Cacciato ◽  
H M. Echavarría ◽  
P Soto
Keyword(s):  
Brucella Abortus ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Bacteriological Culture ◽  
Brucella Suis ◽  
Direct Fluorescent Antibody

<p>El diagnóstico de brucelosis se apoya en el cultivo bacteriológico o en la detección de fragmentos de ADN de la bacteria mediante la reacción en cadena de la polimerasa. El empleo de una inmunoglobulina anti-Brucella conjugada a fluoresceína para la detección de este antígeno en tejidos constituye una técnica simple, fácil, reproducible, económica y rápida. El objetivo de este trabajo fue evaluar la fracción gammaglobulínica de un suero policlonal anti-Brucella abortus marcada con isotiocianato de fluoresceína (FITC), 1) contra distintas especies lisas y rugosas de Brucella sp, 2) contra bacterias de otros géneros, y 3) comparar los resultados obtenidos con la inmunofluorescencia directa y el cultivo bacteriológico para la detección de B. suis en distintos tejidos de porcinos infectados. Este conjugado detectó todas las brucelas con distinta intensidad de fluorescencia, pero no hubo fluorescencia inespecífica cuando se ensayaron las bacterias de otros géneros. La fluorescencia de fondo en muestras de los distintos tejidos infectados fue baja. La mayoría de los tejidos infectados mostraron la presencia de microorganismos verde-fluorescentes con la morfología de las brucelas. El anticuerpo conjugado a FITC permitió un diagnóstico de brucelosis rápido, efectivo y económico.</p>

scholarly journals The direct fluorescent antibody test for detection ofBrucella abortusin bovine abortion material

1973 ◽  
Vol 71 (1) ◽  
pp. 123-129 ◽  
Author(s):  
M. J. Corbel
Keyword(s):  
Brucella Abortus ◽  
Evans Blue ◽  
Q Fever ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Brucella Infection ◽  
Direct Fluorescent Antibody ◽  
Auto Fluorescence ◽  
Bovine Abortion

SUMMARYThe direct fluorescent antibody test was assessed for detection ofBrucella abortusin bovine abortion material.Br. abortusorganisms could be readily detected as characteristic intra-cellular clumps, in smears stained with fluorescent antibody. Counterstaining with Evans Blue was necessary to suppress tissue auto-fluorescence. The method was specific and enabled brucella infection to be differentiated,inter alia, from Q-fever infection.

scholarly journals A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes

2017 ◽  
Vol 9 (01) ◽  
pp. 053-056 ◽  
Author(s):  
Vrushali Patwardhan ◽  
Preena Bhalla ◽  
Deepti Rawat ◽  
Vijay Kumar Garg ◽  
Kabir Sardana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Genital Ulcer ◽  
Chain Reaction ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Simplex Virus

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

Evaluation of the direct fluorescent antibody test for diagnosis of chlamydial infections

1985 ◽  
Vol 4 (6) ◽  
pp. 548-552 ◽  
Author(s):  
K. H. Tjiam ◽  
R. V. W. van Eijk ◽  
B. Y. M. van Heijst ◽  
G. J. Tideman ◽  
Th. van Joost ◽  
...  
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Test ◽  
Direct Fluorescent Antibody ◽  
Chlamydial Infections
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