The direct fluorescent antibody test for the detection of Treponema hyodysenteriae in pigs

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

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Comparative evaluation of direct fluorescent antibody test, Seller's staining test and rapid immunodiagnostic assay for post-mortem diagnosis of rabies in cattle

Indian Journal of Veterinary Pathology ◽

10.5958/0973-970x.2019.00034.8 ◽

2019 ◽

Vol 43 (3) ◽

pp. 165

Author(s):

Monika Thakur ◽

B.S. Sandhu

Keyword(s):

Comparative Evaluation ◽

Fluorescent Antibody ◽

Antibody Test ◽

Post Mortem ◽

Direct Fluorescent Antibody

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Evaluation of Multivalent Leptospira Fluorescent Antibody Conjugates for General Diagnostic Use

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063878900100210 ◽

1989 ◽

Vol 1 (2) ◽

pp. 146-149 ◽

Cited By ~ 28

Author(s):

David A. Miller ◽

Mark A. Wilson ◽

Clyde A. Kirkbride

Keyword(s):

Fluorescent Antibody ◽

Bacterial Species ◽

Antibody Test ◽

Additional Method ◽

Diagnostic Laboratory ◽

Bacterial Cultures ◽

Typical Cell ◽

Antibody Conjugates ◽

Treponema Hyodysenteriae ◽

Culture Positive

Four lots of conjugate were evaluated for optimal dilution and degree of fluorescence produced with reference cultures and bovine and porcine leptospira isolates. One lot that uniformly produced better fluorescence was evaluated for sensitivity and specificity with reference cultures, isolates, culture-positive tissues, and 13 other bacterial species. Further evaluation of the conjugates was done with bovine, porcine, and ovine specimens submitted to a diagnostic laboratory. Leptospires were detected with the fluorescent antibody test (FAT) in 9 of 21 culture-positive bovine kidneys and were detected in diluted cultures when present at concentrations of 102-1O3 organisms/ml. With the exception of Treponema hyodysenteriae, FAT's of other bacterial cultures produced minimal fluorescence or were negative. Positives were characterized by moderate to brilliant fluorescence of typical cell forms, and most nonspecific fluorescence was eliminated with a flazo-orange counter-stain. The results indicated that the FAT utilizing multivalent conjugates could be used successfully as an additional method for diagnosis of leptospira infections.

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