T cell receptor cross-recognition of an HIV-1 CD8+ T cell epitope presented by closely related alleles from the HLA-A3 superfamily

Previous studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine. In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8+T cell epitope, we investigated the influence of multiplying epitope copies on both the frequency and the MFI of specific IFN-γsecreting CD8+T cells. We found that frequencies of specific CD8+T cell could be improved by multiplying epitope copies, while the MFI of IFN-γsecreted by epitope-specific CD8+T cells decreased synchronously. And further analysis showed that the decrease of MFI was not caused by the functional avidity variation of CD8+T cell receptor.

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Highly restricted T-cell receptor repertoire in the CD8+ T-cell response against an HIV-1 epitope with a stereotypic amino acid substitution

AIDS ◽

10.1097/qad.0b013e32832605e6 ◽

2009 ◽

pp. 1 ◽

Cited By ~ 3

Author(s):

Eriko Miyazaki ◽

Ai Kawana-Tachikawa ◽

Mariko Tomizawa ◽

Jun-ichi Nunoya ◽

Takashi Odawara ◽

...

Keyword(s):

Amino Acid ◽

T Cell ◽

T Cell Receptor ◽

Cd8 T Cell ◽

Cell Receptor ◽

T Cell Response ◽

Receptor Repertoire ◽

T Cell Receptor Repertoire ◽

Hiv 1 ◽

Cell Receptor Repertoire

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PEMETAAN EPITOP DAN APLIKASI KLINISNYA

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v17i3.1168 ◽

2018 ◽

Vol 17 (3) ◽

pp. 166

Author(s):

Jusak Nugraha

Keyword(s):

T Cell ◽

T Cell Receptor ◽

Epitope Mapping ◽

Cell Epitope ◽

Cell Receptor ◽

T Cell Epitope ◽

Recombinant Peptide ◽

Protective Epitope ◽

Binding Epitope ◽

Epitope Binding

Epitope mapping is one of the important findings in immunoIogy. The idea of systematic epitope mapping was first described byGeysen et al (Geysen et al., 1987a,b). This technic is further developed together with other important findings such as monoclonalantibody production, DNA recombinant, peptide synthesis and phage display of peptide or protein. The usage of this technic is to know theexact binding site of antigen with antibody or T cell receptor, and can be used as the basic information to design a vaccine or diagnostictools. The term epitope can be further classified as functional epitope, structural epitope, binding epitope, protective epitope, heavyinfection epitope, neutralization epitope etc. In this article will be reviewed topics about epitope: B and T cell epitope mapping technicsusing synthetic pin and its application.

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Breadth of CD8 T-cell mediated inhibition of replication of diverse HIV-1 transmitted-founder isolates correlates with the breadth of recognition within a comprehensive HIV-1 Gag, Nef, Env and Pol potential T-cell epitope (PTE) peptide set

PLoS ONE ◽

10.1371/journal.pone.0260118 ◽

2021 ◽

Vol 16 (11) ◽

pp. e0260118

Author(s):

Peter Hayes ◽

Natalia Fernandez ◽

Christina Ochsenbauer ◽

Jama Dalel ◽

Jonathan Hare ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

Cd8 T Cells ◽

Cell Epitope ◽

T Cell Responses ◽

Cd8 T Cell ◽

T Cell Epitope ◽

Cell Numbers ◽

Cell Responses ◽

Hiv 1

Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral responses, would aid development of effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify T-cell epitopes utilising extensive peptide sets require subjects’ cell numbers that exceed feasible sample volumes. To address this, CD8 T-cells were polyclonally expanded from PBMC from 13 anti-retroviral naïve subjects living with HIV using CD3/CD4 bi-specific antibody. Assessment of recognition of individual peptides within a set of 1408 HIV-1 Gag, Nef, Pol and Env potential T-cell epitope peptides was achieved by sequential IFNγ ELISpot assays using peptides pooled in 3-D matrices followed by confirmation with single peptides. A Renilla reniformis luciferase viral inhibition assay assessed CD8 T-cell-mediated inhibition of replication of a cross-clade panel of 10 HIV-1 isolates, including 9 transmitted-founder isolates. Polyclonal expansion from one frozen PBMC vial provided sufficient CD8 T-cells for both ELISpot steps in 12 of 13 subjects. A median of 33 peptides in 16 epitope regions were recognised including peptides located in previously characterised HIV-1 epitope-rich regions. There was no significant difference between ELISpot magnitudes for in vitro expanded CD8 T-cells and CD8 T-cells directly isolated from PBMCs. CD8 T-cells from all subjects inhibited a median of 7 HIV-1 isolates (range 4 to 10). The breadth of CD8 T-cell mediated HIV-1 inhibition was significantly positively correlated with CD8 T-cell breadth of peptide recognition. Polyclonal CD8 T-cell expansion allowed identification of HIV-1 isolates inhibited and peptides recognised within a large peptide set spanning the major HIV-1 proteins. This approach overcomes limitations associated with obtaining sufficient cell numbers to fully characterise HIV-1-specific CD8 T-cell responses by different functional readouts within the context of extreme HIV-1 diversity. Such an approach will have useful applications in clinical development for HIV-1 and other diseases.

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