Evaluation of the Methicillin-Resistant Staphylococcus aureus (MRSA)-Screen Latex Agglutination Test for Detection of MRSA of Animal Origin

The performance of a second-generation rapid agglutination kit, Slidex Staph Plus (SSP; bioMérieux), was compared to those of the Slidex Staph (SS; bioMérieux), Staphaurex (SRX; Murex Diagnostics), and BBL Staphyloslide (BBL; Becton Dickinson) kits by using 508 clinical isolates composed of 150 methicillin-sensitiveStaphylococcus aureus (MSSA) organisms, 154 methicillin-resistant S. aureus (MRSA) organisms, and 204 non-S. aureus Staphylococcus spp. Of the 508 isolates tested, 75% were fresh clinical isolates, with the remainder taken from five different freezer collections. All four agglutination tests had comparable sensitivities for MSSA and MRSA. However, the SS kit was significantly less specific (93.1%) than the three other tests (P > 0.05, McNemar test). These results demonstrate that the new rapid latex agglutination kit, SSP, was more specific for the identification of S. aureus than the previous version and performed comparably to the SRX and BBL kits.

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Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus

Journal of Clinical Microbiology ◽

10.1128/jcm.37.9.2789-2792.1999 ◽

1999 ◽

Vol 37 (9) ◽

pp. 2789-2792 ◽

Cited By ~ 50

Author(s):

Arjanne van Griethuysen ◽

Miranda Pouw ◽

Nan van Leeuwen ◽

Max Heck ◽

Piet Willemse ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistance ◽

Screen Test ◽

Latex Agglutination ◽

Agglutination Test ◽

Latex Agglutination Test ◽

Specific Test ◽

Methicillin Resistant ◽

Phage Types ◽

Test Specificity

The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test for the determination of methicillin resistance by detection of PBP 2a inStaphylococcus aureus, was compared with the oxacillin agar screen test and PCR detection of the mecA gene. A total of 563 S. aureus isolates were tested. Two hundred ninety-six of the isolates were methicillin-susceptible isolates from cultures of blood from consecutive patients. Also, 267 methicillin-resistant isolates that comprised 248 different phage types were tested. Methicillin resistance was defined as the presence of themecA gene. Of the 267 mecA gene-positive isolates, 263 were positive by the MRSA screen test (sensitivity, 98.5%), and all the mecA-gene negative strains were negative by the MRSA screen test (specificity, 100%). The oxacillin agar screen test detected methicillin resistance in 250 of themecA gene-positive isolates (sensitivity, 93.6%). The sensitivity of the MRSA screen test was statistically significantly higher than the sensitivity of the oxacillin agar screen test (P < 0.05). The MRSA screen test is a highly sensitive and specific test for the detection of methicillin resistance. Also, it offers results within half an hour and is easy to perform, which makes this test a valuable tool in the ongoing battle against methicillin-resistant S. aureus.

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Evaluation of Three Techniques for Detection of Low-Level Methicillin-Resistant Staphylococcus aureus (MRSA): a Disk Diffusion Method with Cefoxitin and Moxalactam, the Vitek 2 System, and the MRSA-Screen Latex Agglutination Test

Journal of Clinical Microbiology ◽

10.1128/jcm.40.8.2766-2771.2002 ◽

2002 ◽

Vol 40 (8) ◽

pp. 2766-2771 ◽

Cited By ~ 128

Author(s):

A. Felten ◽

B. Grandry ◽

P. H. Lagrange ◽

I. Casin

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Latex Agglutination ◽

Agglutination Test ◽

Disk Diffusion ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Methicillin Resistant ◽

Low Level ◽

Vitek 2

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P900 Performance of the GeneXpert ®system for detection of methicillin-resistant Staphylococcus aureus including the non-typeable clone from animal origin

International Journal of Antimicrobial Agents ◽

10.1016/s0924-8579(07)70741-0 ◽

2007 ◽

Vol 29 ◽

pp. S233-S234

Author(s):

M.W.M. Wassenberg ◽

S. Nijssen ◽

X.W. Huijsdens ◽

A.M.C. Bergmans ◽

C.M.J.E. Vandenbroucke-Grauls ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Animal Origin ◽

Methicillin Resistant

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Detection of mecA gene by latex agglutination and its molecular analysis by PCR in methicillin resistant staphylococcus aureus.

The Professional Medical Journal ◽

10.29309/tpmj/2020.27.07.3752 ◽

2020 ◽

Vol 27 (07) ◽

pp. 1363-1370

Author(s):

Aneela Khawaja ◽

Iffat Javed ◽

Sohaila Mushtaq ◽

Saeed Anwar ◽

Faiqa Arshad ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistance ◽

Meca Gene ◽

Latex Agglutination ◽

Medical Institute ◽

Cross Sectional ◽

Methicillin Resistant ◽

Agglutination Method ◽

Resistant Strains

Antimicrobial resistance (AMR) is a devastating question that is threatening the health globally. The extensive and indiscriminative use of antibiotics has evolved a notorious resistance in Staphylococcus aureus. This resistance developed through possession of mecA gene, which codes for modified penicillin binding protein (PBP2a) and the emergent strain being labeled “methicillin resistant Staphylococcus aureus”. Conventional phenotypic techniques for detection of MRSA rely on standardization of cultural characteristics. The drawbacks of diagnostic error to report MRSA include: poor prognosis, expensive treatment, dissemination of multi-drug resistant strains and even treatment failure. Latex agglutination method can be adopted as a more accurate and quick strategy for rapid detection of methicillin resistance. Objectives: To compare detection of mecA gene in methicillin resistant isolates of Staphylococcus aureus by latex agglutination and PCR; by assessing the sensitivity and specificity of both methods. Study Design: Descriptive Cross-Sectional study. Setting: Pathology Department, Post Graduate Medical Institute, Lahore. Period: From January 2015 to December 2015; according to standard operating procedures at Microbiology laboratory. Material & Methods: A total 713 consecutive, non-duplicate isolates of Staphylococcus aureus were processed. Methicillin resistance was determined using cefoxitin (30mg) by Kirby-Bauer method using CLSI guideline (2016), latex agglutination method; and PCR for mecA gene. Results: The results showed that out of 713 Staphylococcus aureus isolates, 92 (12.90%) isolates were resistant to cefoxitin and were labelled as MRSA. majority MRSA isolates recovered from pus (44.57%) and wound swab (20.65%), followed by blood (13.04%), fluid (8.70%), CSF (4.35%), CVP (3.26%), HVS (3.26%) and tracheal secretion (2.17%). By latex agglutination method, 87 (94.50%) were positive for PBP2a; while on PCR mecA gene was detected only in 82 (89.10%) MRSA isolates. When assessed with PCR (gold standard) the sensitivity and diagnostic accuracy of latex agglutination was 100% and 94.57%, respectively. Conclusion: Latex agglutination test can be employed as rapid and reliable diagnostic technique in MRSA isolates for mecA gene detection, where resources for molecular methods are inadequate. This can effectually lessen the misdiagnosis of resistant strains, and over/ ill-use of antibiotics.

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Molecular Characterization and Antimicrobial Resistance Profile of Methicillin-Resistant Staphylococcus aureus in Retail Chicken

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-150 ◽

2015 ◽

Vol 78 (10) ◽

pp. 1879-1884 ◽

Cited By ~ 24

Author(s):

KHALID IBRAHIM SALLAM ◽

SAMIR MOHAMMED ABD-ELGHANY ◽

MOHAMED ELHADIDY ◽

TOMOHIRO TAMURA

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Resistance ◽

Molecular Characterization ◽

Methicillin Resistant Staphylococcus Aureus ◽

Meca Gene ◽

Multidrug Resistant ◽

Animal Origin ◽

Contamination Rate ◽

Methicillin Resistant ◽

Food Of Animal Origin

The emergence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is of increasing interest, raising questions about the presence of MRSA in food of animal origin and potential sources of transmission to humans via the food chain. In this study, the prevalence, molecular characterization, virulence factors, and antimicrobial susceptibility patterns of MRSA isolates from 200 retail raw chicken samples in Egypt were determined. MRSA was detected by positive amplification of the mecA gene in 38% (76 of 200) of chicken samples analyzed. This represents a potential public health threat in Egypt, as this contamination rate seems to be the highest among other studies reported worldwide. Furthermore, genes encoding α-hemolysin (hla) and staphylococcal enterotoxins (sea, seb, and sec) were detected in all of the 288 MRSA isolates. Nonetheless, none of the strains tested carried tst, the gene encoding toxic shock syndrome toxin 1. Antimicrobial resistance of MRSA isolates was most frequently detected against penicillin (93.4%), ampicillin (88.9%), and cloxacillin (83.3%). These results suggest that retail chicken might be a significant potential source for transmission of multidrug-resistant and toxigenic S. aureus in Egypt. This underlines the need for stricter hygienic measures in chicken production in Egypt to minimize the risk of transmission of these strains to consumers. To the best of our knowledge, this is the first study that reports the isolation and molecular characterization of MRSA in retail chicken samples in Egypt.

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