scholarly journals Detection of the plasmid-mediated colistin-resistance genemcr-1in clinical isolates and stool specimens obtained from hospitalized patients using a newly developed real-time PCR assay: Table 1.

2016 ◽  
Vol 71 (8) ◽  
pp. 2344-2346 ◽  
Author(s):  
R. H. T. Nijhuis ◽  
K. T. Veldman ◽  
J. Schelfaut ◽  
A. Van Essen-Zandbergen ◽  
E. Wessels ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Hospitalized Patients ◽  
Clinical Isolates ◽  
Colistin Resistance ◽  
Pcr Assay ◽  
Stool Specimens

scholarly journals Real-Time PCR Assay for Detection of blaZ Genes in Staphylococcus aureus Clinical Isolates

2014 ◽  
Vol 52 (4) ◽  
pp. 1259-1261 ◽  
Author(s):  
L. A. Pereira ◽  
G. B. Harnett ◽  
M. M. Hodge ◽  
J. A. Cattell ◽  
D. J. Speers
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Isolates ◽  
Pcr Assay

Improvement in the detection rate of diarrhoeagenic bacteria in human stool specimens by a rapid real-time PCR assay

2004 ◽  
Vol 53 (7) ◽  
pp. 617-622 ◽  
Author(s):  
Yoshio Iijima ◽  
Nahoko T. Asako ◽  
Masanori Aihara ◽  
Kozaburo Hayashi
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Infections ◽  
Food Poisoning ◽  
Culture Method ◽  
Pcr Analysis ◽  
Laboratory System ◽  
Pcr Assay ◽  
Conventional Culture ◽  
Stool Specimens

A rapid laboratory system has been developed and evaluated that can simultaneously identify major diarrhoeagenic bacteria, including Salmonella enterica, Vibrio parahaemolyticus, Campylobacter jejuni and Shiga toxin-producing Escherichia coli, in stool specimens by real-time PCR. Specific identification was achieved by using selective TaqMan probes, detecting two targets in each pathogen. A positive result was scored only when both targets of a pathogen were amplified and the difference between threshold cycles for detection was less than five. Diagnosis of enteric bacterial infections using this highly sensitive method, including DNA extraction and real-time PCR, requires only 3 h. Forty stool specimens related to suspected food poisoning outbreaks were analysed: 16 (40 %) of these samples were found to be positive for diarrhoeagenic bacteria using a conventional culture method; 28 (70 %) were positive using the real-time PCR assay. Of the 12 PCR-positive but culture-negative cases, 11 patients had consumed pathogen-contaminated or high-risk food. Analysis of faecal samples from 105 outpatients who complained of diarrhoea and/or abdominal pain identified 19 (18 %) patients as being positive for diarrhoeagenic bacteria using the culture method. An additional six (6 %) patients were found to be positive by PCR analysis.

scholarly journals Development of real-time PCR assay allowed describing the first clinical Klebsiella pneumoniae isolate harboring plasmid-mediated colistin resistance mcr-8 gene in Algeria

2020 ◽  
Vol 20 ◽  
pp. 266-271 ◽  
Author(s):  
Larbi Zakaria Nabti ◽  
Farida Sahli ◽  
Edgarthe Priscilla Ngaiganam ◽  
Nadia Radji ◽  
Wahiba Mezaghcha ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
Colistin Resistance ◽  
Pcr Assay
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