Determination of Vanillin by Ultraviolet Absorption

1964 ◽  
Vol 47 (3) ◽  
pp. 555-557 ◽  
Author(s):  
Fraxk J Feexy
Keyword(s):  
Chromatographic Method ◽  
Rapid Determination ◽  
Ultraviolet Absorption ◽  
The Other ◽  
Green Fluorescence ◽  
Ethyl Vanillin ◽  
Alkaline Conditions

Abstract Because the official chromatographic method for vanillin, ethyl vanillin, and coumarin in vanilla extracts is timeconsuming, a sorting procedure based on ultraviolet absorption was developed for rapid determination of vanillin in the absence of the other two components. Small amounts of coumarin are detected by its green fluorescence under alkaline conditions. Thirteen collaborators analyzed four samples for vanillin by the official and the proposed methods; seven checked for the presence of coumarin. On the basis of results, the method is recommended for adoption as official, first action.

Quantitative Determination of Vanillin and Ethyl Vanillin in Flavors by Paper Chromatography

1964 ◽  
Vol 47 (6) ◽  
pp. 1161-1165
Author(s):  
J Fitelson
Keyword(s):  
Quantitative Determination ◽  
Paper Chromatography ◽  
Alkaline Solution ◽  
Chromatographic Separation ◽  
Chromatographic Method ◽  
Ultraviolet Absorption ◽  
Photometric Method ◽  
Absorption Method ◽  
Ethyl Vanillin

Abstract Previous methods for estimating vanillin and ethyl vanillin in mixtures were long and complicated. However, this paper presents a simple chromatographic separation and quantitative determination of these aromatics, using Mitchell equipment for paper chromatography and 8 × 8” papers. Development for 2 hours separates the compounds adequately; they are then extracted and measured by absorbances at 348 mμ in alkaline solution. Recoveries of added vanillin and ethyl vanillin to vanilla extract are excellent. Aside from normal manipulative errors, the only significant inaccuracy is caused by the small amount of natural p-hy-droxybenzaldehyde in vanilla (usually equivalent to about 5% of the vanillin content). The method does not separate p-hydroxybenzaldehyde and vanillin. Total errors in the method do not exceed 0.01%. Comparison of the method with the two AOAC methods for vanillin shows that the paper chromatographic method gives results close to those by the ultraviolet absorption method, in most cases, and significantly lower results than the photometric method, which is known to give erroneously high results.

Gas Chromatographic Determination of Phenolic Compounds in Drug Preparations: Collaborative Study

1972 ◽  
Vol 55 (3) ◽  
pp. 610-612 ◽  
Author(s):  
Carol C Douglas
Keyword(s):  
Phenolic Compounds ◽  
Methyl Salicylate ◽  
Chromatographic Method ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
The Other ◽  
Gas Chromatographic Method

Abstract A gas chromatographic method for the determination of phenol, methyl salicylate, menthol , and camphor in drug preparations was studied collaboratively by 9 laboratories. Mean recoveries were: phenol 98.2, 106.0, a n d 103.6%; methyl salicylate 103.9, 102.0, and 101.9%; menthol 102.9 and 100.9%; and camphor 101.9 and 100.9%. The method has been adopted as official first action for camphor-containing drugs. Additional study will be done to improve recoveries for the other 3 compounds.

scholarly journals Authentication of Straight Whiskey by Determination of the Ratio of Furfural to 5-Hydroxymethyl-2-Furaldehyde

1999 ◽  
Vol 82 (4) ◽  
pp. 997-1001 ◽  
Author(s):  
James Jaganathan ◽  
Sumer M Dugar
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
The Other ◽  
Color Intensity ◽  
Other Hand ◽  
Liquid Chromatographic Method ◽  
Oak Barrels ◽  
Liquid Chromatographic

Abstract Bureau of Alcohol, Tobacco and Firearms regulations require that a straight whiskey be aged in a freshly charred oak barrel for a minimum of 2 years and that it not be colored with added caramel. The regulations, however, permit addition of caramel in blended whiskeys. Blended whiskeys are usually produced by mixing a straight whiskey with neutral spirits which causes loss of color intensity. Caramel addition is permitted to compensate for this loss. Thus, it is not possible to authenticate the standard of identity of a straight whiskey by measurement of color intensity. Our investigations suggest that furfural (2-furaldehyde) and 5-hydroxymethyl-2-furaldehyde are imparted into a straight whiskey during aging in a freshly charred oak barrel. Caramel, on the other hand, imparts only 5-hydroxymethyl-2-furaldehyde. Thus, the measurement of the concentrations of furfural and 5-hydroxymethyl-2-furaldehyde and their ratio could effectively authenticate the standard of identity of straight whiskeys. This study shows that straight whiskeys aged in freshly charred oak barrels for a period of 2 years or more have a 2:1 or higher ratio of furfural to 5-hydroxymethyl-2-furaldehyde. A high-performance liquid chromatographic method for the determination of furfural and 5-hyroxy- methyl-2-furaldehyde at low parts-per-million levels is described.

Improved ultraviolet spectrophotometric method for determination of nitrate in natural waters

2002 ◽  
Vol 2 (2) ◽  
pp. 213-221 ◽  
Author(s):  
N. Kishimoto ◽  
I. Somiya ◽  
R. Taniyama
Keyword(s):  
Water Quality ◽  
Spectrophotometric Method ◽  
Standard Error ◽  
Coefficient Of Variation ◽  
Natural Waters ◽  
Uv Light ◽  
Rapid Determination ◽  
The Other ◽  
Extreme Conditions

An improved UV spectrophotometric method for determination of nitrate in natural waters was proposed and the determination accuracy of the method was discussed in this study. As a result of estimation of the proposed method, adoption of 215 and 220 nm for detection of nitrate and interfering substances resulted in decreasing standard error to about a half of that of a conventional spectrophotometric method. One of the reasons was thought that the coefficient of variation of relative absorbance of interfering substances (rs) at 215 nm was smaller and more stable than that at 275 nm, which was used in the conventional method. The other reason inferred was that most of the interfering substances absorbed UV light at wavelengths of 215 and 220 nm. Although rs has to be renewed when targeted water quality is under extreme conditions, the procedure of the proposed method is very simple, namely only measuring two UV absorbances of a filtrated sample, and more accurate than the conventional spectrophotometric method. Consequently, the proposed method is available for rapid determination of nitrate in natural waters.

Gas-Liquid Chromatographic Quantitative Determination of Vanillin and Ethyl Vanillin in Foods

1974 ◽  
Vol 57 (2) ◽  
pp. 329-331
Author(s):  
Janis E Schlack ◽  
Joseph J Dicecco
Keyword(s):  
Quantitative Determination ◽  
Chromatographic Method ◽  
Methylene Chloride ◽  
Ethyl Vanillin ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A gas-liquid chromatographic method for vanillin and ethyl vanillin in foods has been devised. Vanillin and ethyl vanillin are extracted into methylene chloride, and the extract is purified, concentrated, and then chromatographed on an EGSS-X column. Recovery of vanillin and ethyl vanillin averaged 99.54 and 98.05%, respectively.

Collaborative Study of Spectrophotometric Determination of Hexachlorocyclopentadiene in Technical Chlordane

1966 ◽  
Vol 49 (2) ◽  
pp. 254-257
Author(s):  
Marshall Malina
Keyword(s):  
Spectrophotometric Determination ◽  
Collaborative Study ◽  
Quantitative Measure ◽  
Ultraviolet Absorption ◽  
The Other ◽  
Positive Bias ◽  
Alternative Procedures ◽  
Small Absorption

Abstract Hexachlorocyclopentadiene exhibits strong ultraviolet absorption at 324 mμ, while technical chlordane exhibits only small absorption at this wavelength. The intensity of this absorption is a quantitative measure of the hexachlorocyclopentadiene content. Eight laboratories cooperated in a collaborative study of two alternative procedures on six samples. Results show that one procedure exhibits a positive bias and that the other procedure does not. The precision of both procedures is excellent. The methods are recommended for adoption as official, first action.

Sign in / Sign up

Export Citation Format

Share Document