Microbiological Determination of Thiram

1973 ◽  
Vol 56 (6) ◽  
pp. 1517-1518
Author(s):  
Andre Rappe ◽  
Guy Mauquoy ◽  
Serge Baur
Keyword(s):  
Bacillus Licheniformis ◽  
Agar Plate ◽  
Test Organism ◽  
Test Strain ◽  
Food Preparation ◽  
Plate Method ◽  
Saccharomyces Carlsbergensis ◽  
Dietetic Food

Abstract A rapid and simple agar plate method has been developed for the microbiological determination of thiram, using Saccharomyces carlsbergensis ATCC 9080 as the test organism. With this organism, as little as 25 ng thiram/ well or 200 ng/disk can be detected, well below the levels that can be detected with either Bacillus licheniformis or Flavobacterium as the test strain. The assay has been applied to a dietetic food preparation.

Determination of Antibiotics in Meat Using Bacillus stearothermophilus Spores

1981 ◽  
Vol 44 (3) ◽  
pp. 194-200 ◽  
Author(s):  
M. BIELECKA ◽  
J. D. BALDOCK ◽  
A. W. KOTULA
Keyword(s):  
Sample Size ◽  
Bacillus Stearothermophilus ◽  
Test Organism ◽  
Optimal Concentration ◽  
Antibiotic Residues ◽  
Plate Method ◽  
Assay Medium ◽  
Organism Growth ◽  
Ph Variations

Ten parameters affecting sensitivity, accuracy and simplicity of the diffusion plate method for determining antibiotic residues in meat were evaluated with spores of Bacillus stearothermophilus as the test organism. Eight antibiotics were studied and included penicillin, bacitracin, tetracycline, chlortetracycline, oxytetracycline, streptomycin, erythromycin and neomycin. Sensitivity of the method was most influenced by concentration of inoculum, quantity of assay medium on the plate and sample size. The optimal concentration of inoculum was established as 2 × 105 spores/ml of medium, quantity of the assay medium on plate/100 mm dia., as 6 ml and quantity of sample poured on disc/12.7 mm dia., as 100 μl. The pH of the assay medium was also important to both antibiotic potency and test organism growth. The activity of streptomycin and erythromycin was the most sensitive to pH variations.

scholarly journals Application of casein agar plate method for the determination of protease activity.

Eisei kagaku ◽  
1988 ◽  
Vol 34 (3) ◽  
pp. 241-247 ◽  
Author(s):  
KATSUSHI YOKOTA ◽  
NAOMI FURUSAWA ◽  
TETSUYA ABE ◽  
SEIKO TAKENAKA
Keyword(s):  
Protease Activity ◽  
Agar Plate ◽  
Plate Method

scholarly journals Microbiological Assay for Lomefloxacin in Coated Tablets

2006 ◽  
Vol 89 (4) ◽  
pp. 1077-1079 ◽  
Author(s):  
Greici Cristiani Gomes ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Bacillus Subtilis ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Method Validation ◽  
Test Organism ◽  
Microbiological Assay ◽  
Plate Method ◽  
Relative Standard ◽  
Bacillus Subtilis Atcc

Abstract The validation of a microbiological assay, applying cylinder plate method for determination of the activity of lomefloxacin in coated tablets is described. Using a strain of Bacillus subtilis ATCC 9372 as the test organism, lomefloxacin was measured in concentrations ranging from 2.0 to 8.0 μg/mL. The method validation showed that it is linear (r = 0.9999), precise (relative standard deviation = 1.15%), and accurate (it measured the added quantities). The excipients did not interfere in the determination. It was concluded that the microbiological assay is satisfactory for quantitation of lomefloxacin in tablets.

Collaborative Study of Microbiological Assay of Oxytetracycline in Feeds

1968 ◽  
Vol 51 (3) ◽  
pp. 548-552
Author(s):  
D C Billman ◽  
H Clark
Keyword(s):  
Collaborative Study ◽  
Test Organism ◽  
Plate Method ◽  
Average Recovery ◽  
Extraction Flask ◽  
Average Coefficient ◽  
Similar Materials ◽  
Aoac Method ◽  
Assay Precision

Abstract A collaborative study was made of a modification of the official AOAC method for the determination of oxytetracycline in feeds. This cylinder plate method uses Bacillus cereus var. mycoides, ATCC No. 11778, as the test organism. The modification consists of using a 20 g instead of a 10 g sample, of using an extraction flask instead of a mortar and pestle or a blender, and of filtering the assay solution. The average recovery of oxytetracycline by 12 collaborators was 92.4% with an average coefficient of variation of 13.5%. Assay precision, determined by computing standard deviations on the differences between pairs of similar materials, was estimated to be 7.4% at 10 g/ton and 5.9% at 25 g/ton. The method is recommended for adoption as official, first action.

scholarly journals Microbiological Assay for Cefoxitin Sodium in Dosage Form

2007 ◽  
Vol 90 (2) ◽  
pp. 452-455 ◽  
Author(s):  
Hrida Regina Nunes Salgado ◽  
Greici Cristiani Gomes Tozo
Keyword(s):  
Staphylococcus Epidermidis ◽  
Dosage Form ◽  
Test Organism ◽  
Microbiological Assay ◽  
Plate Method

Abstract A microbiological assay applying the cylinder-plate method is described for determination of the activity of cefoxitin sodium in injectables. Using a strain of Staphylococcus epidermidis ATCC 12226 as the test organism, cefoxitin sodium was measured in concentrations ranging from 50.0 to 200.0 g/mL. The validation showed that the method was linear (r = 0.9998), precise (RSD = 0.81%), and accurate. It was concluded that the microbiological assay is satisfactory for quantitation of cefoxitin sodium in injectables.

scholarly journals Determination of Listeria monocytogenes numbers at less than 10 cfu/g

2017 ◽  
Vol 56 (1) ◽  
pp. 25-30 ◽  
Author(s):  
K. Hunt ◽  
M. Vacelet ◽  
K. Jordan
Keyword(s):  
Listeria Monocytogenes ◽  
Detection Limit ◽  
Agar Plate ◽  
Foodborne Pathogen ◽  
Petri Dish ◽  
Food Samples ◽  
Plate Method ◽  
Pure Cultures ◽  
Response Current

AbstractListeria monocytogenes is a foodborne pathogen that causes a relatively rare foodborne disease called listeriosis, with a high mortality rate of 20%-30% and an undefined dose response. Current European Union regulations permit up to 100 colony-forming units (cfu)/g in food at the end of its shelf life, where the food has been shown not to support the growth of this pathogenic bacterium. Therefore, enumeration of L. monocytogenes at low numbers in food is important. The objective of this study was to reduce the detection limit of L. monocytogenes in food by a factor of 10. The International Organisation for Standardisation (ISO) 11290-2 method for enumeration of L. monocytogenes in food recommends spreading 0.1 mL of a 1:10 dilution of the food on the surface of an agar plate (detection limit 100 cfu/g), or 1.0 mL spread in equal parts on the surface of three agar plates (detection limit: 10 cfu/g). The pour-plate method (using 1 or 10 mL of an appropriate dilution) was compared to the spread-plate method using the ISO-approved chromogenic medium Agar Listeria according to Ottaviani and Agosti (ALOA). Using the pour-plate method, the colony morphology and halo formation were similar to the spread-plate method from pure cultures and inoculated foods. Using the pour-plate method in a 140 mm Petri dish, 10 mL of a 1:10 dilution of food allowed determination of numbers as low as 1 cfu/g. Applying this method, L. monocytogenes in naturally contaminated food samples were enumerated at numbers as low as 1-9 cfu/g.

scholarly journals Comparison of Micellar Electrokinetic Chromatography, Liquid Chromatography, and Microbiologic Assay for Analysis of Cephalexin in Oral Suspensions

2003 ◽  
Vol 86 (4) ◽  
pp. 707-713 ◽  
Author(s):  
Martin Steppe ◽  
María S Aurora Prado ◽  
Marina F M Tavares ◽  
Teresinha J A Pinto ◽  
Erika R M Kedor-Hackmann ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Micellar Electrokinetic Chromatography ◽  
Test Organism ◽  
Electrokinetic Chromatography ◽  
European Pharmacopoeia ◽  
Plate Method ◽  
Commercial Sample ◽  
Relative Standard ◽  
Routine Quality Control

Abstract Two well-accepted methodologies, based on a microbiologic assay (MA) and liquid chromatography (LC), and a novel methodology using micellar electrokinetic chromatography (MEKC), were compared for the determination of cephalexin in commercially available and simulated samples of oral suspensions. The MA, described in the BrazilianPharmacopeia, was performed with a strain of Staphylococcus aureus ATCC 6538 as the test organism, following the cylinder-plate method. The LC analysis followed the European Pharmacopoeia, 3rd Ed., and was used with minor modifications. The MEKC analysis was based on a previous work of the group. Estimates of the repeatability relative standard deviations of the MA, LC, and MEKC methods in the analysis of a commercial sample were 0.34, 0.42, and 0.37%, respectively. The recovery obtained with LC was 99.90 ± 1.11%; for MEKC, it was 100.09 ± 0.56%. Although the 3 methodologies were statistically equivalent for the determination of cephalexin in oral suspensions, MA gave suitable repeatability despite being nonspecific and time-consuming. MEKC provided faster analysis and higher column efficiency, whereas LC presented superior sensitivity. The results indicated that MEKC can be used as an alternative method to MA and LC in routine quality control laboratories.

scholarly journals Validation Parameters as Applied to Methods for Quantification of Microorganisms in Medicinal Products

2020 ◽  
Vol 10 (4) ◽  
pp. 267-272
Author(s):  
I. A. Buylova ◽  
O. V Gunar
Keyword(s):  
Quantitative Determination ◽  
Agar Plate ◽  
Test Method ◽  
Medicinal Products ◽  
Plate Method ◽  
Microbiological Methods ◽  
Study Results ◽  
Validation Parameters ◽  
Limit Of Quantitation

Validation/verification of microbiological methods is a prerequisite for quality control of non-sterile drugs. However, the use of existing procedures for validation/verification of analytical methods is challenging, since a number of factors, such as microorganism distribution in the sample, cell morphology, and metabolic activity of microorganisms contribute to the error in microbiological testing.The aim of the study was to assess the feasibility of using the microbiological method validation parameters for validation/verification of the agar plate method.Materials and methods: 18 non-sterile medicinal products were used in the study. Experiments included determination of antimicrobial activity. The quantification of viable bacteria, yeasts and moulds was performed using the modified pour plate method. The statistical processing of the obtained results was performed using Microsoft Excel 7.0 and Statistica 8.0.Results: the paper provides the results of quantitative determination of test microorganisms inoculated into non-sterile drugs. The results were obtained as part of validation/verification of the agar plate method of the State Pharmacopoeia of the Russian Federation, XIV ed.Conclusions: the validation/verification of the test method for isolation and quantification of microorganisms revealed no deviations of the study results from the established acceptance criteria. This proves the feasibility of using the following validation parameters: accuracy, precision, robustness, and limit of quantitation when validating new methods for quantitative determination of microorganisms or verification of previously validated methods.

scholarly journals Azide Esculin Agar Plate Method for Determination of Enterococci in Frozen Foods

1974 ◽  
Vol 15 (2) ◽  
pp. 105-109 ◽  
Author(s):  
Susumu HORIE ◽  
Shiro SATO ◽  
Toru MORITA ◽  
Hiroshi INOUE ◽  
Tsutomu IZUMI ◽  
...  
Keyword(s):  
Agar Plate ◽  
Frozen Foods ◽  
Plate Method
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