Reverse Phase High Pressure Liquid Chromatographic Determination of Vitamin K1 in Infant Formulas

1983 ◽  
Vol 66 (5) ◽  
pp. 1063-1066
Author(s):  
Martin P Bueno ◽  
Melina C Villalobos
Keyword(s):  
High Pressure ◽  
Chromatographic Determination ◽  
Hplc Method ◽  
Vitamin K1 ◽  
Infant Formulas ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A reverse phase high pressure liquid chromatographic (HPLC) method for quantitating vitamin K1 in enzymatic hydrolysates of infant formula is described. The vitamin is extracted with n-pentane before determination by isocratic and isothermal reverse phase HPLC. Recovery of vitamin Ki added io 5 infant formulas ranged from 84 to 103%

High Pressure Liquid Chromatographic Determination of Monensin in Feed Premixes

1983 ◽  
Vol 66 (2) ◽  
pp. 284-286
Author(s):  
Thomas D Macy ◽  
Andrew Loh
Keyword(s):  
High Pressure ◽  
Coefficient Of Variation ◽  
Chromatographic Determination ◽  
Hplc Method ◽  
High Pressure Liquid Chromatographic ◽  
Average Recovery ◽  
Bioassay Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic (HPLC) method has been developed to determine monensin in feed premixes. The method is simple and rapid. Monensin is extracted with methanol-water and determined in the extracting solution by HPLC. Average recovery for monensin from a 13.2% premix sample was 103% (coefficient of variation (CV), 2.6%) by HPLC and compares with the value of 100% (CV, 3.4%) obtained by the turbidimetric bioassay method.

High Pressure Liquid Chromatographic Determination of Oxazepam Dosage Forms: Collaborative Study

1983 ◽  
Vol 66 (4) ◽  
pp. 864-866
Author(s):  
Eileen S Bargo ◽  
◽  
E Aranda ◽  
C Bonnin ◽  
S Hauser ◽  
...  
Keyword(s):  
High Pressure ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A reverse phase high pressure liquid chromatographic method for the determination of oxazepam in tablets and capsules was collaboratively studied by 9 laboratories. Collaborators were supplied with 6 samples that included synthetic and commercial formulations. Tablet and capsule composites are diluted with methanol and filtered. Oxazepam is determined at 254 nm by using a C18 column. Mean recoveries of oxazepam from synthetic tablet and capsule formulations were 97.2 and 99.0%, respectively. Mean coefficients of variation for tablets and capsules ranged from 1.85 to 2.86%. The method has been adopted official first action.

High Pressure Liquid Chromatographic Determination of Physostigmine Salicylate and Physostigmine Sulfate in Liquids and Ointments: Collaborative Study

1982 ◽  
Vol 65 (1) ◽  
pp. 132-137
Author(s):  
Norlin W Tymes ◽  
◽  
G Briguglio ◽  
C Corcoran ◽  
R Everett ◽  
...  
Keyword(s):  
High Pressure ◽  
Collaborative Study ◽  
Internal Standard ◽  
Pharmaceutical Formulations ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Results of 11 laboratories are presented for the collaborative study of a proposed method for the quantitative reverse phase high pressure liquid chromatographic (HPLC) determination of physostigmine salicylate and physostigmine sulfate in pharmaceutical formulations. The samples consisted of commercial solution, injection, and ointment preparations, each containing one of the physostigmine salts. The physostigmine salt is extracted from ointments with acetonitrile after the ointment is dissolved in hexane. Liquid preparations are diluted directly. Physostigmine is determined at 254 nm on a C18 column by comparison with a physostigmine standard. Flurazepam hydrochloride is the internal standard. The method has been adopted official first action for the solution dosage form.

High Pressure Liquid Chromatographic Determination of Antihistamine-Adrenergic Combination Products

1980 ◽  
Vol 63 (1) ◽  
pp. 91-94 ◽  
Author(s):  
William J Bachman
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Chromatographic Determination ◽  
Step Response ◽  
Chlorpheniramine Maleate ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Reverse phase high pressure liquid chromatography (HPLC) with ion-pairing is used for the determination of pseudoephedrine hydrochloride in combination with triprolidine hydrochloride or chlorpheniramine maleate in sirups and tablets. Sirups require a preliminary column chromatography cleanup step. Response is linear for pseudoephedrine hydrochloride (range of 0–20 μg), chlorpheniramine maleate (range of 0–1.3 μg), and triprolidine hydrochloride (range of 0–1.0 μg). Recoveries from synthetic formulations were 98.8–101.3% for pseudoephedrine hydrochloride, 100.0–101.2% for chlorpheniramine maleate, and 97.7–99.8% for triprolidine hydrochloride. The coefficient of variation for the method is less than 1%.

High Pressure Liquid Chromatographic Determination of Theobromine and Caffeine in Cocoa and Chocolate Products

1978 ◽  
Vol 61 (6) ◽  
pp. 1424-1427 ◽  
Author(s):  
Wesley R Kreiser ◽  
Robert A Martin
Keyword(s):  
High Pressure ◽  
Chromatographic Determination ◽  
Hot Water ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Hot Water Extraction ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method was developed for determining theobromine and caffeine in cocoa and chocolate products by high pressure liquid chromatography. After a simple hot water extraction, both theobromine and caffeine were separated by using a reverse phase Cl s column and a mobile phase of methanol-water-acetic acid (20+ 79+1). Theobromine and caffeine were quantitated at 280 nm; average recoveries were 98.7 and 95.0%; and coefficients of variation were 2.31 and 3.91%, respectively.

High Pressure Liquid Chromatographic Determination of Sucrose in Honey

1977 ◽  
Vol 60 (4) ◽  
pp. 838-841 ◽  
Author(s):  
James E Thean ◽  
Walter C Funderburk
Keyword(s):  
High Pressure ◽  
Membrane Filter ◽  
Chromatographic Determination ◽  
Hplc Method ◽  
High Pressure Liquid Chromatographic ◽  
Field Samples ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Refractive Index Detector

Abstract A normal phase high pressure liquid chromatographic (HPLC) method is presented for separating and determining sucrose in honeys. This method has been successfully applied to the analysis of field samples containing sucrose (0.63–8.44 wt %). Diluted honeys are filtered through a 0.45 μm membrane filter, and injected directly into the chromatograph. Samples are eluted from a μBondapak/carbohydrate column with acetonitrile-water (83+17) and quantitated with a refractive index detector. Average recovery of sucrose is 97%.

High Pressure Liquid Chromatographic Determination of Allantoin in Cosmetic Creams and Lotions

1982 ◽  
Vol 65 (6) ◽  
pp. 1362-1365
Author(s):  
Kazuo Nakao ◽  
Keiichi Honda ◽  
Tohru Yoneya
Keyword(s):  
High Pressure ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Silica Column ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A sensitive high pressure liquid chromatographic method was developed for the determination of allantoin in cosmetic preparations. The procedure consists of simple cleanup of samples, derivatization with p-nitrobenzaldehyde in N,N-dimethylformamide to an ultraviolet labeled derivative, and reverse phase chromatography on an octadecylsilylated silica column. Ultraviolet absorbance was measured at 270 nm. Recovery was greater than 97% for cosmetic samples, and the minimum limit of detection was 10 ng.

High Pressure Liquid Chromatographic Determination of Uncombined Intermediates and Subsidiary Colors in Orange B

1977 ◽  
Vol 60 (5) ◽  
pp. 1067-1069
Author(s):  
Manjeet Singh
Keyword(s):  
High Pressure ◽  
Thin Layer ◽  
Sulfonic Acid ◽  
Chromatographic Determination ◽  
Hplc Method ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Good Agreement

Abstract A high pressure liquid chromatographic (HPLC) method has been developed for isolating and determining uncombined intermediates and subsidiary colors in Orange B. Samples of Orange B containing 0.1–0.3% naphthionic acid, 0.05–0.2 % phenylhydrazine-p-sulfonic acid, 0.2–0.8% pyrazolone-T and ethyl ester of pyrazolone-T, 0.2–1.0% 3-[(4-sulfo-l-naphthalenyl)-azo]-4-amino-l-naphthalenesulfonic acid, and 0.1–6.0% Orange K were prepared and analyzed by using this method. Recoveries ranged from 95 to 103%, except for the phenylhydrazine-p-sulfonic acid which ranged from 95 to 140%. Ten samples of Orange B were analyzed by conventional column and thin layer chromatographic methods as well as by the HPLC method. Good agreement was obtained for naphthionic acid, Orange K, and naphthionic acid plus the naphthionic acid subsidiary.

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