Liquid Chromatographic Determination of Benzoyl Peroxide in Acne Preparations
Keyword(s):
Abstract A rapid, precise, and accurate liquid chromatographic (LC) method is described for the determination of benzoyl peroxide (BP) in acne preparations. BP is extracted from a water dispersion of the preparation with dichloromethane (DCM), and an aliquot is eluted from a C-18 reverse phase LC column with acetonitrile-O.lOM aqueous NaCI04. Selective and sensitive quantitation is accomplished with a reductive mode electrochemical detector. This detector is an order of magnitude more sensitive than a 240 nm UV absorption detector; the lower limit of detection is 2 ng for a 4 μL injection. The recovery of BP is 99.4% and the detector response is linear to at least 2 μg per 4 μL injection.
1994 ◽
Vol 77
(3)
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pp. 752-755
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1988 ◽
Vol 71
(5)
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pp. 1068-1071
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1988 ◽
Vol 71
(5)
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pp. 957-961
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1984 ◽
Vol 67
(5)
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pp. 861-862
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