Modification of the Rapid Screening Method for Aflatoxin in Corn for Quantitative Use

Abstract A study was made to determine if the official AOAC method for screening of aflatoxin in corn could be modified for use as a quantitative method. Several different corn products were analyzed using the modified method, with an average savings of over 1 h/sample vs the CB method. Average recoveries for aflatoxin Bt were 94% for the low level spiked samples and 108% for the high level. Samples of corn and corn products containing naturally incurred aflatoxin were also analyzed with the modified method, and the results compared favorably with those obtained by the CB method.

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Modified 1–2 Test™ System as a Rapid Screening Method for Detection of Salmonella in Foods and Feeds

Journal of Food Protection ◽

10.4315/0362-028x-53.8.656 ◽

1990 ◽

Vol 53 (8) ◽

pp. 656-658 ◽

Cited By ~ 12

Author(s):

JOHN J. OGGEL ◽

DEV. C. NUNDY ◽

CHESLEY J. RANDALL

Keyword(s):

Positive Result ◽

Screening Method ◽

Culture Method ◽

Rapid Screening ◽

Standard Culture ◽

Rapid Screening Method ◽

Time Required ◽

High Level ◽

Level Of Agreement ◽

Culture Procedure

Modifications made to the procedure for the 1–2 Test™ System (BioControl Systems, Inc., 19805 North Creek Parkway, Bothell, WA 98011) resulted in reducing the time required for analysis by 24 h, while maintaining a high level of agreement with the standard culture procedure. Of 283 samples tested, 73 (25.8%) were found to be naturally contaminated with Salmonella by all analytical conditions combined. The standard culture procedure detected 73 of the contaminated samples, whereas the 1–2 Test™ system, as utilized in this study, found 70 samples to be positive for Salmonella. The modified 1–2 Test™ System procedure yielded a presumptive positive result after 48 h, compared to the 96 h required with the standard culture method, and 72 h with the 1–2 Test™ System as previously evaluated in this laboratory (8).

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Modified Rapid Screening Method for Aflatoxin in Corn

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/56.4.1024 ◽

1973 ◽

Vol 56 (4) ◽

pp. 1024-1025

Author(s):

Gail M Shannon ◽

Robert D Stubblefield ◽

Odette L Shotwell

Keyword(s):

Ammonium Sulfate ◽

Lead Acetate ◽

Screening Method ◽

Saturated Solution ◽

Rapid Screening ◽

Modified Method ◽

Yellow Corn ◽

Acetone Water ◽

Rapid Screening Method

Abstract A rapid screening method for detecting aflatoxin in cottonseed products was investigated to determine if it was applicable to corn. Other aflatoxin-extracting solvents and protein-precipitating agents were tried with white and yellow corn. When acetone-water (85+15) and a saturated solution of ammonium sulfate were substituted for acetonitrile-water (80+20) and lead acetate in the cottonseed method, fewer interferences and more compact fluorescent zones were observed on the developed minicolumns. The modified method for corn was applicable to samples containing 10 ppb or more aflatoxin.

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Confirmation of Results of Rapid Screening Test for Aflatoxins Performed at Corn Elevator

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/59.6.1419 ◽

1976 ◽

Vol 59 (6) ◽

pp. 1419-1421

Author(s):

Odette L Shotwell ◽

Gail M Shannon ◽

Marion L Goulden

Keyword(s):

Screening Test ◽

Animal Feed ◽

Screening Method ◽

Rapid Screening ◽

Total Aflatoxin ◽

Corn Products ◽

Rapid Screening Test ◽

Aoac Method

Abstract A screening method for corn and corn products, based on a minicolumn, was modified slightly to assay 60 lots of corn at one elevator to determine whether they could be sold as animal feed. To be salable, the lots had to contain less than 20 ppb total aflatoxin. Aflatoxin levels in the lots were later determined by the official AOAC method for corn to check effectiveness of the screening. No lot had been designated for sale that contained 20 or more ppb total aflatoxin.

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Rapid Screening Method for Aflatoxins and Zearalenone in Corn

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/58.1.114 ◽

1975 ◽

Vol 58 (1) ◽

pp. 114-116 ◽

Cited By ~ 1

Author(s):

Fred Thomas ◽

Robert M Eppley ◽

Mary W Trucksess

Keyword(s):

Aflatoxin B1 ◽

Screening Method ◽

Water Extract ◽

Water Extraction ◽

Rapid Screening ◽

Extraction System ◽

Copper Carbonate ◽

Elapsed Time ◽

Aoac Method ◽

Rapid Screening Method

Abstract The methanol-water extraction system used in AOAC Method II for aflatoxins extracts both the aflatoxins and zearalenone from corn. Using this methanol-water extraction system as a base, a rapid screening procedure has been developed for these mycotoxins. The methanol-water extract is defatted with hexane and the pigments are precipitated with copper carbonate. The aflatoxins and zearalenone are subsequently extracted into chloroform and are then detected by half-plate TLC. An elapsed time of about 1 hr is required to analyze 1 sample. The sensitivity of the method is about 2 μg/kg for aflatoxin B1 and 100 μg/kg for zearalenone.

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