Production of Antibodies and Development of Enzyme Immunoassay for Determination of Monensin in Biological Samples

1987 ◽  
Vol 70 (2) ◽  
pp. 201-205
Author(s):  
Michael E Mount ◽  
Douglas L Failla
Keyword(s):  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Biological Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coefficients Of Variation ◽  
Highly Sensitive

Abstract Monensin is converted to monensin bromoacetate, which provides successful coupling to protein and allows production of monensinspeciflc rabbit antisera. A modified indirect enzyme-linked immunosorbent assay (ELISA) was developed, which is highly sensitive (2 ng/mL) to monensin determination. Monensin recovery was 53- 81% at 10 ppb in sera or urine, and 81-130% at 100 ppb in dichloromethane- extracted feces or water. Overall recovery was 98.8% with coefficients of variation from 6 to 52% over the range of monensin concentrations studied. This is the first immunoassay reported for the carboxylic ionophore antibiotics.

Determination of Four Nitrofuran Metabolites and Chloramphenicolin Biological Samples Using Enzyme-Linked Immunosorbent Assay

2013 ◽  
Vol 46 (9) ◽  
pp. 1404-1418 ◽  
Author(s):  
Jianzhong Shen ◽  
Wenjun Wang ◽  
Xi Xia ◽  
Jinghui Zhu ◽  
Xiaoping Wu ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Biological Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nitrofuran Metabolites

scholarly journals Determination of Egg Proteins in Food Products by Enzyme Immunoassay

2000 ◽  
Vol 83 (1) ◽  
pp. 139-143 ◽  
Author(s):  
Jupiter M Yeung ◽  
W Harvey Newsome ◽  
Michael A Abbott
Keyword(s):  
Detection Limit ◽  
Enzyme Immunoassay ◽  
Polyclonal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Binding ◽  
Food Ingredients ◽  
Coefficients Of Variation ◽  
Egg Products ◽  
Whole Egg

Abstract An enzyme-linked immunosorbent assay (ELISA) was developed to determine the presence of egg proteins in foods. The polyclonal antibodies developed were specific to whole egg proteins and did not cross-react with any of the 38 nuts, legumes, or other common food ingredients tested. The concentrations of egg proteins that will inhibit 50% of antibody–antigen binding, IC50, were 3–7 ng/mL, and the linear range was 0.5–62.5 ng/mL. The detection limit was 0.2 ppm for various foods. Recoveries ranged from 67 to 96%. The intra- and inter-assay coefficients of variation in this procedure were 10–13% for ice cream spiked at 0.8 and 1.6 ppm. The ELISA has been applied to ice creams, noodles, pasta, and breads. Egg proteins were identified in all declared egg products, and no false positives were found.

scholarly journals Simultaneous Determination of Florfenicol and Its Metabolite Florfenicol Amine in Swine Muscle Tissue by a Heterologous Enzyme-Linked Immunosorbent Assay

2009 ◽  
Vol 92 (3) ◽  
pp. 981-988 ◽  
Author(s):  
Pengjie Luo ◽  
Haiyang Jiang ◽  
Zhanhui Wang ◽  
Caimao Feng ◽  
Fangyang He ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Muscle Tissue ◽  
Immunosorbent Assay ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A sensitive and heterologous enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of florfenicol (FF) and its metabolite florfenicol amine (FFA) in swine muscle tissue was developed. FFA was conjugated to bovine serum albumin by a formaldehyde coupling method as an immunogen to immunize rabbits. FFA, thiamphenicol glycinate, and modified FF were conjugated to ovalbumin as coating antigens. The effect of different types of hapten heterology on the sensitivity and specificity of the ELISA was evaluated. Using FF glutaric anhydride ester as a coating hapten and antibody raised against modified FFA, an ELISA was developed that showed an IC50 value of 0.48 ng/mL. The antibody showed a cross-reactivity of 100 with FFA, 97 with FF, 6 with thiamphenicol, and a negligible value with chloramphenicol. From fortified swine muscle samples at levels of 4320 ng/g, the average recoveries of FF and FFA ranged from 58.2 to 96.8, with coefficients of variation less than 14. Analysis of incurred samples by the ELISA gave similar results to those by a previously developed liquid chromatographic method. The ELISA could be used as a rapid method for the simultaneous determination of FF and FFA in swine muscle tissue.

Determination of Zearalenone and Related Metabolites in Porcine Urine by Modified Enzyme-Linked Immunosorbent Assay

1990 ◽  
Vol 73 (1) ◽  
pp. 65-68
Author(s):  
Ormond A Macdougald ◽  
Andrew J Thulin ◽  
James J Pestka
Keyword(s):  
Immunosorbent Assay ◽  
Excretion Rate ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Linear Portion ◽  
Average Recovery ◽  
Standard Curve ◽  
Coefficients Of Variation ◽  
Peroxidase Conjugate

Abstract A direct competitive enzyme-linked immunosorbent assay (ELISA) was modified for the determination of zearalenone and zearalenols in porcine urine. In the modified procedure, standard or unknown concentrations of zearalenone were added to all wells, followed by rapid addition of zearalenonehorseradlsh peroxidase conjugate, whereas in previous methods, zearalenone and zearalenone-horseradish peroxidase were mixed and then added to microtiter wells. The modification increased the number of urine samples that could be analyzed per assay. The linear portion of the modified ELISA standard curve covered the range of 10-500 ng zearalenone/mL. Average recovery of zearalenone from spiked urine was 91,101,95,107,136,112, and 120% for 1, 10, 25, 50, 100, 250, and 500 ng zearalenone/mL urine, respectively. Mean within-assay coefficient of variation for each concentration of zearalenone in 5 standard curves was 5.95%. Between-assay coefficients of variation for concentrations of zearalenone equivalents in lower and upper regions of the standard curve were 10.9% (n = 18) and 8.8% (n = 20), respectively. Analysis of urine samples showed that a gilt excreted 5.3% of ingested zearalenone in the 8 h following ingestion, with an average excretion rate of about 100 /ug zearalenone equivalents/h.

A new residue method for the determination of flonicamid in agricultural and environmental samples using enzyme immunoassay systems

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35842-35846 ◽  
Author(s):  
Zhenjiang Liu ◽  
Kewei Ren ◽  
Ming Li ◽  
Jiagao Wang ◽  
Jianfan Sun ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Environmental Samples ◽  
Enzyme Linked Immunosorbent Assay

An indirect competitive enzyme-linked immunosorbent assay (ELISA) for flonicamid was developed based on a polyclonal antibody.

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