A new residue method for the determination of flonicamid in agricultural and environmental samples using enzyme immunoassay systems

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35842-35846 ◽  
Author(s):  
Zhenjiang Liu ◽  
Kewei Ren ◽  
Ming Li ◽  
Jiagao Wang ◽  
Jianfan Sun ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Environmental Samples ◽  
Enzyme Linked Immunosorbent Assay

An indirect competitive enzyme-linked immunosorbent assay (ELISA) for flonicamid was developed based on a polyclonal antibody.

Highly selective and sensitive determination of deoxymiroestrol using a polyclonal antibody-based enzyme-linked immunosorbent assay

Talanta ◽  
2013 ◽  
Vol 114 ◽  
pp. 73-78 ◽  
Author(s):  
Gorawit Yusakul ◽  
Orapin Udomsin ◽  
Thaweesak Juengwatanatrakul ◽  
Hiroyuki Tanaka ◽  
Chaiyo Chaichantipyuth ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sensitive Determination

scholarly journals Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S199-S202 ◽  
Author(s):  
M. Vítková ◽  
Z. Macková ◽  
R. Koblovská ◽  
O. Lapčík
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Medicago Sativa ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Bovine Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acceptable Range ◽  
Carboxy Methyl

The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4’ via a carboxy methyl spacer. The developed ELISAs are highly specific, I<sub>50</sub> values of the standard curves range between 0.3–1.2 ng/ml. The cross reactivities to other isoflavones are in acceptable range and the interference of non-isoflavonoid molecules is negligible. The immunoassays have been used for monitoring of changes in isoflavone levels in alimentary important plants, such as Medicago sativa, during germination; and during different vegetation stages of the Rutaceae family plants.

Production of Antibodies and Development of Enzyme Immunoassay for Determination of Monensin in Biological Samples

1987 ◽  
Vol 70 (2) ◽  
pp. 201-205
Author(s):  
Michael E Mount ◽  
Douglas L Failla
Keyword(s):  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Biological Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coefficients Of Variation ◽  
Highly Sensitive

Abstract Monensin is converted to monensin bromoacetate, which provides successful coupling to protein and allows production of monensinspeciflc rabbit antisera. A modified indirect enzyme-linked immunosorbent assay (ELISA) was developed, which is highly sensitive (2 ng/mL) to monensin determination. Monensin recovery was 53- 81% at 10 ppb in sera or urine, and 81-130% at 100 ppb in dichloromethane- extracted feces or water. Overall recovery was 98.8% with coefficients of variation from 6 to 52% over the range of monensin concentrations studied. This is the first immunoassay reported for the carboxylic ionophore antibiotics.

Simultaneous detection of parathion and imidacloprid using broad-specificity polyclonal antibody in enzyme-linked immunosorbent assay

2015 ◽  
Vol 7 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Lening Zhang ◽  
Zhanfeng Wang ◽  
Yubin Wen ◽  
Jingwei Shi ◽  
Jincheng Wang
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Simultaneous Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Broad Specificity

A heterologous indirect competitive enzyme-linked immunosorbent assay for the gross determination of parathion and imidacloprid residues was developed.

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