Determination of Cymiazole Residues in Honey by Liquid Chromatography

1993 ◽  
Vol 76 (1) ◽  
pp. 92-94 ◽  
Author(s):  
Paolo Cabras ◽  
Marinella Melis ◽  
Lorenzo Spanedda
Keyword(s):  
Liquid Chromatography ◽  
Chromatographic Analysis ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Limit Of Determination ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Control Samples

Abstract A liquid chromatographic method is described for the determination of cymiazole residues in honey. This acaricide is determined on a reversed-phase (C18) column, with a CH3CN-O.OOIN HCI-NaCI mixture (950 mL + 50 mL + 0.3 g/L) as the mobile phase, and UV detection at 265 nm. Cymiazole is extracted with n-hexane from aqueous alkalinized (pH 9) honey solutions. No further cleanup of the honey extract was required before chromatographic analysis. Recoveries on control samples fortified with 0.01,0.10, and 1.00 ppm cymiazole ranged from 92 to 102%. The limit of determination was 0.01 ppm.

scholarly journals Micellar liquid chromatographic analysis of benzyl alcohol and benzaldehyde in injectable formulations

2007 ◽  
Vol 57 (2) ◽  
pp. 231-239 ◽  
Author(s):  
Mohamed Rizk ◽  
Fawzia Ibrahim ◽  
Mohamed Hefnawy ◽  
Jenny Nasr
Keyword(s):  
Benzyl Alcohol ◽  
Simultaneous Determination ◽  
Chromatographic Analysis ◽  
Chromatographic Method ◽  
Limit Of Detection ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Micellar liquid chromatographic analysis of benzyl alcohol and benzaldehyde in injectable formulationsAn accurate, sensitive and selective reversed-phase micellar liquid chromatographic method was developed for simultaneous determination of benzyl alcohol and benzaldehyde. This method was applied in different injectable formulations containing diclofenac, piroxicam, lincomycin and clindamycin. The method showed excellent linearity in the range of 10-100 μg mL-1and 1-20 μg mL-1with the limit of detection (S/N = 3) 0.25 μg mL-1(2.3 x 10-6mol L-1) and 0.12 μg mL-1(1.13 x 10-6mol L-1) for benzyl alcohol and benzaldehyde, respectively. The suggested method was successfully applied to the analysis of the studied drugs in bulk with average recoveries of 100.1 ± 1.0% for benzyl alcohol and 100.4 ± 1.6% for benzaldehyde, and to the determination of benzyl alcohol and benzaldehyde in injectable formulations with the respective average recoveries of 99.8 ± 0.3 and 100.0 ± 0.4%.

Simultaneous determination of catecholamines and serotonin by liquid chromatography, after treatment with boric acid gel.

1988 ◽  
Vol 34 (3) ◽  
pp. 528-530 ◽  
Author(s):  
Y Imai ◽  
S Ito ◽  
K Maruta ◽  
K Fujita
Keyword(s):  
Liquid Chromatography ◽  
Boric Acid ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Internal Standards ◽  
Column Temperature ◽  
Liquid Chromatographic Method ◽  
Acid Gel ◽  
Liquid Chromatographic

Abstract We describe a liquid-chromatographic method for simultaneously determining norepinephrine, epinephrine, dopamine, and serotonin in 0.5 mL of human plasma. These analytes are purified on boric acid gel from Aldrich, separated on a reversed-phase C18 column, and detected electrochemically at +600 mV. Absolute recoveries of internal standards were 84% for 3,4-dihydroxybenzylamine and 57% for N-methylserotonin. Reproducibility was good to excellent, depending on the concentration of the analytes. A chromatographic run is complete in 40 min, but this can be shortened by about half when the determination of only serotonin is required, by increasing the column temperature from 40 degrees C to 60 degrees C.

scholarly journals Development and Validation of Liquid Chromatographic Method for Estimation of Ibuprofen and Famotidine in Combined Dosage Form

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Dimal A. Shah ◽  
Dixita J. Suthar ◽  
Sunil L. Baldania ◽  
Usman K. Chhalotiya ◽  
Kashyap K. Bhatt
Keyword(s):  
Mobile Phase ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Assay Method ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Development And Validation ◽  
Liquid Chromatographic

An isocratic, reversed phase-liquid-chromatographic assay method was developed for the quantitative determination of ibuprofen and famotidine in combined-dosage form. A Brownlee C18, 5 μm column with mobile phase containing water : methanol : acetonitrile (30 : 60 : 10, v/v/v) was used. The flow rate was 1.0 mL/min, and effluents were monitored at 264 nm. The retention times of ibuprofen and famotidine were 4.9 min and 6.8 min, respectively. The linearity for ibuprofen and famotidine was in the range of 2–20 μg/mL and 0.1–10 μg/mL, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. The method was successfully applied to the estimation of ibuprofen and famotidine in combined dosage form.

Liquid Chromatographic Determination of Barbaloin (Aloin) in Foods

1985 ◽  
Vol 68 (3) ◽  
pp. 493-494
Author(s):  
Masatoshi Yamamoto ◽  
Masaaki Ishikawa ◽  
Toshio Masui ◽  
Hiroyuki Nakazawa ◽  
Yozo Kabasawa
Keyword(s):  
Liquid Chromatography ◽  
Present Method ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Food Samples ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple and rapid liquid chromatographic method is described for the determination of barbaloin (aloin, 10-D-gIucopyranosyl-l,8-dihydroxy- 3-(hydroxymethyI)-9(10#)-anthracenone) in foods. Barbaloin is extracted with water from foods containing aloe and the extract is cleaned up on a disposable cartridge by using methanol-water (55 + 45) as eluant. The eluted barbaloin is separated by liquid chromatography on a YMC A-302 column with methanol-water (50 + 50) mobile phase, and detected at 293 nm. Recoveries of barbaloin added to foods at the levels of 0.05 and 0.50 mg/g were 94.4-100%. Assay results for commercial food samples indicated that the present method is applicable to a variety of foods supplemented with aloe.

A Green Liquid Chromatographic Method For The Simultaneous Determination of Chlorpheniramine Maleate, Pseudoephedrine Hydrochloride and Propyphenazone

2019 ◽  
Vol 15 (6) ◽  
pp. 635-641
Author(s):  
Nadia M. Mostafa ◽  
Ghada M. Elsayed ◽  
Nagiba Y. Hassan ◽  
Dina A. El Mously
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Green Analytical Chemistry ◽  
Chlorpheniramine Maleate ◽  
Accuracy And Precision ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background:The concept of green analytical chemistry prevails due to the growing environmental pollution.Objective:Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form.Methods:Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min.Results:Quantification was done over the concentration ranges of 1-25 µg/mL for chlorpheniramine maleate, 5-35 µg/mL for pseudoephedrine hydrochloride and 10-120 µg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results.Conclusion:It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

Liquid Chromatographic Method for Determination of Dithianon in Technical Products and Formulations: Collaborative Study

1995 ◽  
Vol 78 (5) ◽  
pp. 1131-1133
Author(s):  
Alan R Hanks
Keyword(s):  
Chromatographic Method ◽  
Collaborative Study ◽  
Reversed Phase ◽  
Study Data ◽  
Water Detection ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Liquid Chromatographic ◽  
Technical Products

Abstract A liquid chromatographic method for determination of dithianon in technical products and formulations was evaluated by 22 laboratories participating in a collaborative study. Data received from 19 laboratories were used in the statistical analysis. The study included 3 technical grade materials, 1 suspension concentrate, and 1 wettable powder. Dithianon is determined by reversed-phase liquid chromatography on a C18 column with a mobile phase of acetonitrile and water. Detection at 254 nm with peak area measurements is used with an external standard for quantitation of dithianon. The liquid chromatographic method for determination of dithianon in technical products and formulations has been adopted first action by AOAC INTERNATIONAL.

Sign in / Sign up

Export Citation Format

Share Document