Determination of Semduramicin Sodium in Poultry Liver by Liquid Chromatography with Vanillin Postcolumn Derivatization

1994 ◽  
Vol 77 (3) ◽  
pp. 577-582 ◽  
Author(s):  
Jon F Ericson ◽  
Albert Calcagni ◽  
Martin J Lynch
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Solid Phase ◽  
Ammonium Hydroxide ◽  
Phase Extraction ◽  
Minimum Level ◽  
The Mean ◽  
Liquid Chromatographic ◽  
Postcolumn Derivatization

Abstract A liquid chromatographic (LC) method is described for the quantitative determination of semduramicin sodium in broiler liver when administered under projected use conditions. For this procedure, semduramicin sodium is extracted from liver with methanolic ammonium hydroxide, separated and concentrated by solid-phase extraction steps, and determined by LC with postcolumn derivatization with vanillin. The mean recovery of drug was 95% over the 40-320 ng/g range, the coefficient of variation was ±10% or better, and no interference was observed from commercial poly ether ionophores. The minimum level of detection for semduramicin sodium in broiler liver is 25 ng/g.

scholarly journals Solid-Phase Extraction and Gas Chromatography with Mass Spectrometric Determination of Capsaicin and Some of Its Analogues from Chili Peppers (Capsicum spp.)

1999 ◽  
Vol 82 (6) ◽  
pp. 1399-1405 ◽  
Author(s):  
Philemon Manirakiza ◽  
Adrian Covaci ◽  
Paul Schepens
Keyword(s):  
Gas Chromatography ◽  
Quantitative Determination ◽  
Solid Phase ◽  
Accurate Method ◽  
Mass Spectrometric ◽  
Phase Extraction ◽  
Capsicum Spp ◽  
The Mean ◽  
Chili Peppers

Abstract A rapid and accurate method has been developed for the quantitative determination of capsaicin and its most important analogues, dihydrocapsaicin and nordihydrocapsaicin in chili peppers. These components were extracted with methylene chlo ride and separated from interfering substances with activated charcoal. Further cleanup on Florisil cartridges and elution with ethyl acetate were performed before gas chromatographic with mass spectrometric quantitation. The concentrations found were 440 ± 64 μg/g capsaicin, 81 ± 10 μg/g dihydrocapsaicin, and 11 ± 2 μg/g nordihydrocapsaicin. The mean recovery values for triplicate analysis were between 85-94%.

Determination of Nitroimidazole Metabolites in Swine and Turkey Muscle by Liquid Chromatography

1992 ◽  
Vol 75 (5) ◽  
pp. 790-795 ◽  
Author(s):  
Edward T Mallinson ◽  
A Carolyn Henry ◽  
Loyd Rowe
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Extraction Method ◽  
Solid Phase ◽  
Phase Extraction ◽  
Recovery Curves ◽  
External Standard ◽  
The Mean ◽  
Initial Extraction

Abstract The method described can determine hydroxy dimetridazole and hydroxy ipronidazole in turkey and swine muscle tissue at levels of 2 ppb. After an initial extraction with ethyl acetate and subsequent evaporation, the samples are solvated in acid and back-extracted. A C18 solid-phase extraction is performed after neutralization of the acidic extract. The basis for quantitation was an external standard containing both analytes. After performing 10 recovery curves with blank control tissue spiked at 0, 1,2, and 4 ppb, the mean recoveries of hydroxy dimetridazole and hydroxy ipronidazole were 61.8 and 64.6%, respectively. Recovery variation was less than 20% for both analytes over all 10 curves. The performance of the extraction method was verified in tissues incurred through the dosing of live animals.

Determination of Novobiocin Residues in Milk, Blood, and Tissues by Liquid Chromatography

1988 ◽  
Vol 71 (4) ◽  
pp. 776-778 ◽  
Author(s):  
William A Moats ◽  
Laura Leskinen
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase ◽  
Solvent Mixture ◽  
Simple Liquid ◽  
Phase Extraction ◽  
Aqueous Methanol ◽  
Final Injection ◽  
Wavelength Detector ◽  
Liquid Chromatographic

Abstract Residues of novobiocin in milk, blood, and tissues can be detected by microbiological tests but cannot be distinguished from other antibiotics. A simple liquid chromatographic (LC) method was developed for identification of residues. Tissues were blended and milk and blood serum were mixed with 0.2M NH4H2P04. The mixture was deproteinized by adding aqueous methanol and filtering. The LC apparatus consisted of a variable wavelength detector, set at 340 nm, an automatic loop injector, and a C,8 column with guard cartridge. The flow rate was 1 mL/min and the solvent mixture of 0.01M H3P04-acetonitrile-methanol was programmed from 50 + 0 + 50 (0-1 min) to 20 + 80 + 0 (20 min). Novobiocin was concentrated directly by solid-phase extraction on the analytical column. Five or more 200 11L aliquots of the filtrate in water-methanol (1 + 1) (adjusted if necessary) were injected with the column solvent at 50 + 0 + 50. After the final injection, the program was run to completion. Recoveries were 90-100% with sensitivities of 0.05 ppm or less. The procedure should be adaptable for use with formulations and feeds

Sign in / Sign up

Export Citation Format

Share Document