scholarly journals Thin-Layer Chromatographic Detection of Phosphorothionate and Phosphorothiolothionate Pesticides Using 4-Amino-N,N-Diethylaniline

1996 ◽  
Vol 79 (4) ◽  
pp. 1009-1011 ◽  
Author(s):  
Akmal Pasha ◽  
Yadathora N Vuayashankar ◽  
Nitturu G K Karanth
Keyword(s):  
Thin Layer ◽  
Chromatographic Method ◽  
Limit Of Detection ◽  
Chromogenic Reagent ◽  
Highly Sensitive ◽  
Subsequent Exposure ◽  
Chromatographic Detection ◽  
Bromine Vapor

Abstract A thin-layer chromatographic method using a novel chromogenic reagent was developed to detect the phosphorothionate and phosphorothiolothionate groups of pesticides. On reaction with 4-am'mo-N,N-diethylaniline and subsequent exposure to bromine vapor, these compounds yield a deep magenta product. The chromogenic reagent is specific to these organophosphates and gives no response to phosphorothiolates and substituted phosphonates. The method is rapid and highly sensitive. The limit of detection is 0.05-0.5 μg.

scholarly journals Validated HPTLC Method for Quantification of Luteolin and Apigenin inPremna mucronataRoxb., Verbenaceae

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Nayan G. Patel ◽  
Kalpana G. Patel ◽  
Kirti V. Patel ◽  
Tejal R. Gandhi
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Methanolic Extract ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reflection Mode ◽  
Limit Of Quantitation ◽  
Hptlc Method

A simple, rapid, and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin inPremna mucronataRoxb., family Verbenaceae. Separation was performed on silica gel 60 F254HPTLC plates using toluene : ethyl acetate : formic acid (6 : 4 : 0.3) as mobile phase for elution of markers from extract. The determination was carried out in fluorescence mode using densitometric absorbance-reflection mode at 366 nm for both luteolin and apigenin. The methanolic extract ofPremna mucronatawas found to contain 10.2 mg/g % luteolin and 0.165 mg/g % of apigenin. The method was validated in terms of linearity, LOD and LOQ, accuracy, precision, and specificity. The calibration curve was found to be linear between 200 and 1000 ng/band for luteolin and 50 and 250 ng/band for apigenin. For luteolin and apigenin, the limit of detection was found to be 42.6 ng/band and 7.97 ng/band while the limit of quantitation was found to be 129.08 ng/band and 24.155 ng/band, respectively. This developed validated method is capable of quantifying and resolving luteolin and apigenin and can be applicable for routine analysis of extract and plant as a whole.

Sensitive Thin Layer Chromatographic Detection of High Fructose Corn Sirup and Other Adulterants in Honey

1979 ◽  
Vol 62 (4) ◽  
pp. 917-920 ◽  
Author(s):  
Irene Kushnir
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Plant Source ◽  
High Fructose ◽  
Highly Sensitive ◽  
Rf Values ◽  
Chromatographic Detection ◽  
Total Mixture ◽  
Layer Chromatography

Abstract A highly sensitive procedure has been developed to detect the undeclared addition of high fructose corn sirup (HFCS) to honey. Carbohydrates must be separated first to achieve the requisite degree of sensitivity: charcoal-Celite chromatography was used to isolate a fraction containing oligo- and polysaccharides. The fraction was then concentrated and examined by thin layer chromatography on silica gel. Pure honeys yielded only 1 or 2 blue-grey or bluebrown spots at Rf values >0.35; a series of spots or blue streaks extending from the origin characterized adulterated samples. The method detects HFCS and conventional honey adulterants at levels as low as 10% or less of the total mixture. In addition, the procedure detects the presence in honey of all starch-derived sugar sirups tested thus far, regardless of the plant source.

Thin Layer Chromatographic Detection and Spectrophotometric Determination of the Trisodium Salt of 1,3,6-PyrenetrisuIfonic Acid in D&C Green No. 8

1984 ◽  
Vol 67 (4) ◽  
pp. 762-763
Author(s):  
Naomi Richfield-Fratz
Keyword(s):  
Thin Layer ◽  
Spectrophotometric Determination ◽  
Chromatographic Method ◽  
Chromatographic Detection ◽  
Trisodium Salt

Abstract A thin layer chromatographic method is presented for separating the reaction by-product 1,3,6-pyrenetrisuIfonic acid (trisodium salt) (PTS) from D&C Green No. 8 (8-hydroxy-l,3,6-pyrenetrisulfonic acid). PTS is detected visually, extracted from the adsorbent, and determined spectrophotometrically. Recoveries of PTS added at 0.75-6.73% levels to 8-hydroxy-l,3,6-pyrenetrisuIfonic acid ranged from 80.0 to 94.8%.

Thin Layer Chromatographic Detection of Carum bulbocastanum Koch in Carum carvi Linn

1977 ◽  
Vol 60 (1) ◽  
pp. 235-236
Author(s):  
Asit R Sen ◽  
Pranab K Sardar ◽  
Pratima Sengupta
Keyword(s):  
Thin Layer ◽  
Essential Oils ◽  
Petroleum Ether ◽  
Silica Gel ◽  
Chromatographic Method ◽  
Carum Carvi ◽  
Chromatographic Detection

Abstract A rapid thin layer chromatographic method has been developed to detect Carum bulbocastanum Koch in Carum carvi Linn. The volatile essential oils are developed on silica gel G plates, using benzene-petroleum ether and visualized by 2,4-dinitrophenylhydrazine spray. This procedure can also differentiate mixtures of the 2 seeds.

Chromatographic Detection of Flavor Additives in Vanilla Extract

1965 ◽  
Vol 48 (3) ◽  
pp. 507-508
Author(s):  
S Kahan ◽  
J Fitelson
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Chromatographic Method ◽  
Collaborative Study ◽  
Layer Method ◽  
Thin Layer Method ◽  
Chromatographic Detection

Abstract A second collaborative study was conducted on a thin-layer chromatographic method for the detection of flavor additives, adopted as official, first action last year. Fifteen collaborators reported successfully on the presence of added flavoring materials in fovir unknown samples. Some collaborators found significant variations in the activity of the Silica Gel G used to make the thin-layer plates, and a precautionary statement has been inserted in the method to detect svich inactive or overactive reagents. As a result of this study, the thin-layer method has been recommended for adoption as official, final action.

scholarly journals Development and validation of a simple thin-layer chromatographic method for the analysis of p-chlorophenol in treated wastewater

2012 ◽  
Vol 77 (11) ◽  
pp. 1649-1659
Author(s):  
Maja Natic ◽  
Dragana Dabic ◽  
Dusanka Milojkovic-Opsenica ◽  
Biljana Dojcinovic ◽  
Goran Roglic ◽  
...  
Keyword(s):  
Thin Layer ◽  
Advanced Oxidation Processes ◽  
Chromatographic Method ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Treated Wastewater ◽  
Sensitivity Limit ◽  
Limit Of Quantification ◽  
Wastewater Samples ◽  
Development And Validation

A thin-layer chromatographic method with densitometric detection was established for quantification of p-chlorophenol in waste water. Degradation efficiency of p-chlorophenol was monitored after each treatment of the wastewater samples. Degradation of p-chlorophenol was performed with advanced oxidation processes (AOPs), using UV, H2O2/UV, O3/H2O2/UV, O3 and O3/UV. Developed TLC procedure has been found to be simple, rapid and precise. The method was characterized by high sensitivity (limit of detection was 11 ng per band and limit of quantification 35 ng per band), linear range (from 75 to 500 ng per band, r = 0.9965), and high precision, accuracy (mean percentage recovery 98.6%), and specificity. Additionally, the efficiency of degradation was monitored using HPLC giving comparable results with RP TLC measurements.

Thin Layer Chromatographic Detection of Amomum subulatum Roxb in Elletaria cardamomum Maton

1977 ◽  
Vol 60 (2) ◽  
pp. 307-308
Author(s):  
Asit R Sen ◽  
Pratima Sengupta ◽  
Pranab K Sardar ◽  
Bidhan R Roy
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Chromatographic Method ◽  
Solvent System ◽  
Antimony Trichloride ◽  
The Other ◽  
Volatile Oils ◽  
Chromatographic Pattern ◽  
Chromatographic Detection ◽  
Amomum Subulatum

Abstract A thin layer chromatographic method has been developed that distinguishes and differentiates true cardamom (Elletaria cardamomum Maton) and greater cardamom (Amomum subulatum Roxb). Extracted volatile oils are chromatographed on silica gel G with n-hexanediethyl ether (80+20) solvent system and visualized with a saturated solution of antimony trichloride in chloroform. The chromatographic pattern permits the detection of adulteration of one by the other at levels as low as 5%.

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