Investigation of the process of processing blackcurrant and cranberry alcoholic juices with trisodium salt of nitrilotrimethylphosphonic acid

На основании проведенных исследований установлено, что обработка черносмородинового и клюквенного спиртованных соков нитрилотриметилфосфоновой кислотой позволяет снизить концентрацию железа до нормы, установленной требованиями ТР ТС 029/2012 «Требования безопасности пищевых добавок, ароматизаторов и технологических вспомогательных средств» - не более 10 мг/дм, и повысить прозрачность спиртованных соков. Based on the conducted studies, it was found that the treatment of blackcurrant and cranberry alcoholic juices with nitrilotrimethylphosphonic acid reduces the concentration of iron to the norm established by the requirements of Customs Union Technical Regulations «Safety requirements of food additives, flavorings and processing aids» (CU TR 029/2012) - no more than 10 mg/dm and increases the transparency of alcoholic juices.

Rapid Adsorption of Acid Dyes Using Cu(II) Thiourea Modified Cellulose Complex

In the present work, the acid dyes namely, eriochrome cyanine R (ECR) and 2-(4-Sulfo phenyl azo)-1,8 dihydroxy-3,6 naphthalene disulfonic acid, trisodium salt (SPADNS) were effectively adsorbed by Cu(II)-thiourea modified cotton fibers (Cu(II)/Tu-MC) complex. FTIR, SEM, thermogravimetric analysis, and potentiometric titration were utilized for characterization. The impact of the fundamental adsorption parameters was systematically investigated. The results reveal that the adsorption of ECR and SPADNS acid dyes occurs via a metal-coordination mechanism. Furthermore, the adsorption process follows the 2nd order kinetic model and Langmuir model adsorption isotherm. The Cu(II)/Tu-MC shows high adsorption capacities of 0.27 and 0.22 mmol. g-1 for ECR and SPADNS, respectively. These findings indicate that the cationization of cellulose fibers with metal ions is a promising and efficient strategy towards enhancing the adsorption of acid dyes.

Rapid Adsorption of Acid Dyes Using Cu(II) Thiourea Modified Cellulose Complex

In the present work, the acid dyes namely, eriochrome cyanine R (ECR) and 2-(4-Sulfo phenyl azo)-1,8 dihydroxy-3,6 naphthalene disulfonic acid, trisodium salt (SPADNS) were effectively adsorbed by Cu(II)-thiourea modified cotton fibers (Cu(II)/Tu-MC) complex. FTIR, SEM, thermogravimetric analysis, and potentiometric titration were utilized for characterization. The impact of the fundamental adsorption parameters was systematically investigated. The results reveal that the adsorption of ECR and SPADNS acid dyes occurs via a metal-coordination mechanism. Furthermore, the adsorption process follows the 2nd order kinetic model and Langmuir model adsorption isotherm. The Cu(II)/Tu-MC shows high adsorption capacities of 0.27 and 0.22 mmol. g-1 for ECR and SPADNS, respectively. These findings indicate that the cationization of cellulose fibers with metal ions is a promising and efficient strategy towards enhancing the adsorption of acid dyes.

Effect of Chitosan on the Activity of Water-Soluble and Hydrophobic Porphyrin Photosensitizers Solubilized by Amphiphilic Polymers

In this work, we studied the photocatalytic activity of photosensitizers (PSs) of various natures solubilized with polyvinylpyrrolidone (PVP) and ternary block copolymer ethylene and propylene oxide Pluronic F127 (F127) in a model reaction of tryptophan photo-oxidation in water in the presence of chitosan (CT). Water-soluble compounds (dimegin and trisodium salt of chlorin e6 (Ce6)) and hydrophobic porphyrins (tetraphenylporphyrin (TPP) and its fluorine derivative (TPPF20)) were used as PSs. It was shown that the use of chitosan (Mw ~100 kDa) makes it possible to obtain a system whose activity is comparable to that of the photosensitizer-amphiphilic polymer systems. Thus, the previously observed drop in the photosensitizing activity of PS in the presence of a polysaccharide and amphiphilic polymers (AP) was absent in this case. At the same time, chitosan had practically no inhibitory effect on hydrophobic porphyrins solubilized by Pluronic F127.

Development of the methods for determination of the active components in acidic and alkaline detergents

The goal of the study is to develop methods for determination of the active components in acidic and alkaline detergents of complex composition. The objects of analysis are acidic (KATELON 202 – 208) and alkaline (KATELON 111 – 115) detergents of complex composition, which, in addition to easily detectable components (KOH and/or NaOH, HNO3, and H3PO4), contain weak organic acids (lactic, glycolic, acetic), including polybasic acids — aminotrimethylenephosphonic and diethylenetriaminepenta (methylenephosphonic) acids or salts — tetrasodium salt of glutamic acid N,N-diacetate, trisodium salt of methylglycine diacetic acid. An acid-base potentiometric titration was carried out for each of the listed objects and calculation formulas were derived to determine the concentration of the active substances (except for KATELON 115) in terms of nitric or phosphoric acid for acidic detergents or sodium or potassium hydroxide for alkaline detergents, respectively. A specific titration procedure (with indication of the sample weight, the pH value at the end of titration and corresponding calculation formula) is given in the application data sheet for each detergent along with the metrological characteristics of the methods.

Positive feedback between retinoic acid and 2-phospho-L-ascorbic acid trisodium salt during somatic cell reprogramming

Retinoic acid (RA) and 2-phospho-L-ascorbic acid trisodium salt (AscPNa) promote the reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells. In the current studies, the lower abilities of RA and AscPNa to promote reprogramming in the presence of each other suggested that they may share downstream pathways at least partially. The hypothesis was further supported by the RNA-seq analysis which demonstrated a high-level overlap between RA-activated and AscPNa activated genes during reprogramming. In addition, RA upregulated Glut1/3, facilitated the membrane transportation of dehydroascorbic acid, the oxidized form of L-ascorbic acid, and subsequently maintained intracellular L-ascorbic acid at higher level and for longer time. On the other hand, AscPNa facilitated the mesenchymal-epithelial transition during reprogramming, downregulated key mesenchymal transcriptional factors like Zeb1 and Twist1, subsequently suppressed the expression of Cyp26a1/b1 which mediates the metabolism of RA, and sustained the intracellular level of RA. Furthermore, the different abilities of RA and AscPNa to induce mesenchymal-epithelial transition, pluripotency, and neuronal differentiation explain their complex contribution to reprogramming when used individually or in combination. Therefore, the current studies identified a positive feedback between RA and AscPNa, or possibility between vitamin A and C, and further explored their contributions to reprogramming.

Effects of CGA-N12 on the membrane structure of Candida tropicalis cells

The antimicrobial peptide CGA-N12 (NH2-ALQGAKERAHQQ-COOH) is an active peptide derived from chromogranin A (CGA) and consists of the 65th to 76th amino acids of the N-terminus. The results of our previous studies showed that CGA-N12 exerts anti-Candida activity by inducing apoptosis without destroying the integrity of cell membranes. In this study, the effect of CGA-N12 on the cell membrane structure of Candida tropicalis was investigated. CGA-N12 resulted in the dissipation of the membrane potential, the increase in membrane fluidity, and the outflow of potassium ions in C. tropicalis without significantly changing the ergosterol level. Fluorescence quenching was applied to evaluate the membrane channel characteristics induced by CGA-N12 through detection of the following: membrane permeability of hydrated Cl− (ϕ ≈ 0.66 nm) using the membrane-impermeable halogen anion-selective fluorescent dye lucigenin, passage of the membrane-impermeable dye carboxyfluorescein (CF) (ϕ ≈ 1 nm) through the membrane, and membrane permeation of H3O+ based on the membrane non-permeable pH-sensitive fluorescent dye 8-hydroxypyrene-1,3,6-trisulfonic acid, trisodium salt (HPTS). In conclusion, CGA-N12 can induce the formation of non-selective ion channels <1 nm in diameter in the membranes of C. tropicalis, resulting in the leakage of potassium ions, chloride ions, and protons, among others, leading to dissipation of the membrane potential. As a result, the fluidity of membranes is increased without destroying the synthesis of ergosterol is not affected.