scholarly journals Determination of Phenolic Sympathomimetic Drugs in Pharmaceutical Samples and Biological Fluids by Flow-Injection Chemiluminescence

2000 ◽  
Vol 83 (6) ◽  
pp. 1299-1305 ◽  
Author(s):  
Fatma A Aly ◽  
Salma A Al-Tamimi ◽  
Abdulrahman A Alwarthan
Keyword(s):  
Formic Acid ◽  
Flow Injection ◽  
Reaction Pathway ◽  
Biological Fluids ◽  
Dosage Forms ◽  
Pharmaceutical Samples ◽  
Sympathomimetic Drugs ◽  
Highly Sensitive ◽  
Flow Injection Chemiluminescence

Abstract A rapid and highly sensitive flow-injection chemiluminometric method was developed for determination of 3 sympathomimetic drugs, namely, etilefrine hydrochloride, isoxsuprine hydrochloride, and prenalterol hydrochloride. The method is based on chemiluminescence induced by oxidation of drugs with acidified potassium permanganate in the presence of formic acid as a carrier. The calibration graphs were linear over the concentration ranges 0.2–9, 0.2–12.5, and 0.025–1.25 μg/mL for the 3 compounds, respectively. The method was applied successfully in determining the drugs in dosage forms and in biological fluids. A proposal for the reaction pathway is suggested.

scholarly journals Spectrofluorimetric Determination of Vigabatrin and Gabapentin in Dosage Forms and Spiked Plasma Samples Through Derivatization with 4-Chloro-7-Nitrobenzo-2-Oxa-1,3-Diazole

2001 ◽  
Vol 84 (4) ◽  
pp. 1017-1024 ◽  
Author(s):  
Ekram M Hassan ◽  
Fathalla Belal ◽  
Omar A Al-Deeb ◽  
Nasr Y Khalil
Keyword(s):  
Reaction Pathway ◽  
Dosage Forms ◽  
Specific Method ◽  
Plasma Samples ◽  
Spiked Plasma ◽  
Highly Sensitive ◽  
Percent Recovery ◽  
Spectrofluorimetric Determination ◽  
Label Claim

Abstract A highly sensitive and specific method is proposed for the determination of vigabatrin (I) and gabapentin (II) in their dosage forms and spiked human plasma. The method is based on coupling the drugs with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole in borate buffer at pH 7.1 and measuring the resulting fluorescence at 532 nm after excitation at 465 nm. The fluorescence intensity was a linear function of the concentration of the drugs over the ranges of 1.3–6.5 and 1.7–8.5 μg/mL for I and II, respectively. Minimum detectability values were 0.54 μg/mL (4.2 × 10−6M) and 0.97 μg/mL (5.7 × 10−6M) for I and II, respectively, under the described conditions. The proposed method was successfully applied to the determination of the 2 drugs in their dosage forms, and the percent recoveries ± standard deviation (SD) were 104.53 ± 1.2 and 100.00 ± 1.32 of the label claim for I and II, respectively. The method was further applied to the determination of vigabatrin in spiked plasma samples. The percent recovery ± SD was 101.58 ± 2.68. Interference from endogenous α-amino acids was overcome through selective complexation with freshly prepared Cu(OH)2. The interference likely to be encountered from co-administered drugs, such as carbamazepine, cimetidine, clonazepam, clopazam, phenobarbital, valproic acid, and lamotrigine, was also studied. A reaction pathway is suggested.

Flow-injection chemiluminometric determination of some phenothiazines in dosage forms and biological fluids

1998 ◽  
Vol 358 (3) ◽  
pp. 255-262 ◽  
Author(s):  
Fatma A. Aly ◽  
Nawal A. Alarfaj ◽  
Abdulrahman A. Alwarthan
Keyword(s):  
Flow Injection ◽  
Biological Fluids ◽  
Dosage Forms

scholarly journals Micellar Enhanced Spectrofluorometric Determination of Labetalol Through Complexation with Aluminium(III): Application to Dosage Forms and Biological Fluids

2007 ◽  
Vol 90 (4) ◽  
pp. 948-956 ◽  
Author(s):  
Nahed El-Enany
Keyword(s):  
Fluorescence Intensity ◽  
Reaction Pathway ◽  
Biological Fluids ◽  
Sodium Dodecyl ◽  
Dosage Forms ◽  
Spectrofluorometric Determination ◽  
Experimental Parameters ◽  
Fluorescent Products ◽  
Good Agreement

Abstract Two simple, sensitive, and specific spectrofluorometric procedures have been developed for the determination of labetalol (LBT) in pharmaceuticals and biological fluids. LBT was found to react with Al3+ , both in acetate buffer of pH 4.5 (Procedure I) and borate buffer of pH 8.0 (Procedure II), to produce highly fluorescent stable complexes. The fluorescence intensity could be enhanced by the addition of sodium dodecyl sulfate, resulting in 3.5- and 2.7-fold increases in the fluorescence intensity for Procedures I and II, respectively. In both procedures, the fluorescence intensity was measured at 408 nm after excitation at 320 nm. The different experimental parameters affecting the development and stability of the fluorescent products were carefully studied and optimized. The fluorescence intensity-concentration plots were rectilinear over the range of 0.020.1 and 0.010.05 g/mL with a detection limit of 0.003 and 0.001 g/mL for Procedures I and II, respectively. The proposed method was successfully applied to commercial tablets containing LBT. The results were in good agreement with those obtained using a reference spectrofluorometric method. Furthermore, the method was applied for the determination of LBT in spiked human plasma, and the recovery (n = 4) was 93.30 2.62%. A proposal of the reaction pathway was postulated for Procedures I and II, respectively.

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