scholarly journals A Square-Wave Adsorptive Stripping Voltammetric Method for the Determination of Amaranth, a Food Additive Dye

2005 ◽  
Vol 88 (3) ◽  
pp. 788-793 ◽  
Author(s):  
Ahmad H Alghamdi
Keyword(s):  
Soft Drink ◽  
Supporting Electrolyte ◽  
Pulse Amplitude ◽  
Food Additive ◽  
Experimental Conditions ◽  
Analytical Methodology ◽  
Square Wave ◽  
Carbonate Buffer ◽  
Relative Standard

Abstract Square-wave adsorptive stripping voltammetric (AdSV) determinations of trace concentrations of the azo coloring agent Amaranth are described. The analytical methodology used was based on the adsorptive preconcentration of the dye on the hanging mercury drop electrode, followed by initiation of a negative sweep. In a pH 10 carbonate supporting electrolyte, Amaranth gave a well-defined and sensitive AdSV peak at −518 mV. The electroanalytical determination of this azo dye was found to be optimal in carbonate buffer (pH 10) under the following experimental conditions: accumulation time, 120 s; accumulation potential, 0.0 V; scan rate, 600 mV/s; pulse amplitude, 90 mV; and frequency, 50 Hz. Under these optimized conditions the AdSV peak current was proportional over the concentration range 1 × 10−8 − 1.1 × 10−7 mol/L (r = 0.999) with a detection limit of 1.7 × 10−9 mol/L (1.03 ppb). This analytical approach possessed enhanced sensitivity, compared with conventional liquid chromatography or spectrophotometry and it was simple and fast. The precision of the method, expressed as the relative standard deviation, was 0.23%, whereas the accuracy, expressed as the mean recovery, was 104%. Possible interferences by several substances usually present as food additive azo dyes (E110, E102), gelatin, natural and artificial sweeteners, preservatives, and antioxidants were also investigated. The developed electroanalyticals method was applied to the determination of Amaranth in soft drink samples, and the results were compared with those obtained by a reference spectrophotometric method. Statistical analysis (paired t-test) of these data showed that the results of the 2 methods compared favorably.

scholarly journals A Square-Wave Adsorptive Stripping Voltammetric Method for Determination of Fast Green Dye

2009 ◽  
Vol 92 (6) ◽  
pp. 1714-1719
Author(s):  
Ali F Al-Ghamdi
Keyword(s):  
Soft Drink ◽  
Supporting Electrolyte ◽  
Pulse Amplitude ◽  
Fast Green ◽  
Experimental Conditions ◽  
Analytical Methodology ◽  
Square Wave ◽  
Carbonate Buffer ◽  
Enhanced Sensitivity

Abstract Square-wave adsorptive stripping voltammetric (SW-AdSV) determinations of trace concentrations of the coloring agent fast green were described. The analytical methodology used was based on the adsorptive preconcentration of the dye on the hanging mercury drop electrode, and then a negative sweep was initiated. In pH 10 carbonate supporting electrolyte, fast green gave a well-defined and sensitive SW-AdSV peak at 1220 mV. The electroanalytical determination of this dye was found to be optimized in carbonate buffer (pH 10) with the following experimental conditions: accumulation time (120 s); accumulation potential (0.8 V); scan rate (800 mV/s); pulse amplitude (90 mV); frequency (90 Hz); surface area of the working electrode (0.6 mm2); and the convection rate (2000 rpm). Under these optimized conditions, the AdSV peak current was proportional over the concentration range 2 1086 107 M (r = 0.999), with an LOD of 1.63 1010 M (0.132 ppb). This analytical approach possessed more enhanced sensitivity than conventional chromatography or spectrophotometry, and was simple and quick. The precision of the method in terms of RSD was 0.17, whereas the accuracy was evaluated via the mean recovery of 99.6. Possible interferences by several substances usually present as food additive azo dyes (E110, E102, E123, and E129), natural and artificial sweeteners, and antioxidants were also investigated. Applicability of the developed electroanalysis method was illustrated via the determination of fast green in ice cream and soft drink samples.

scholarly journals Determination of Allura Red in Some Food Samples by Adsorptive Stripping Voltammetry

2005 ◽  
Vol 88 (5) ◽  
pp. 1387-1393 ◽  
Author(s):  
Ahmad H Alghamdi
Keyword(s):  
Limit Of Detection ◽  
Soft Drink ◽  
Supporting Electrolyte ◽  
Reduction Process ◽  
Food Additive ◽  
Allura Red ◽  
Food Dye ◽  
Relative Standard ◽  
Voltammetric Peak

Abstract Square wave (SW) voltammetry was used to explore the adsorption properties of the food additive dye Allura Red on a hanging mercury drop electrode (HMDE). By using the adsorptive stripping voltammetric approach, we developed a sensitive electroanalytical method for the determination of this azo dye. A well-developed voltammetric peak probably related to the cathodic reduction of the azo moiety was obtained in pH 9 Britton-Robinson (B-R) buffer at 613 mV. Cyclic voltammetric studies indicated that the reduction process was irreversible and primarily controlled by adsorption. The adsorptive voltammetric signal was evaluated with respect to various experimental conditions; the optimized values were supporting electrolyte, B-R buffer; pH 11; accumulation time, 180s; accumulation potential, 0.0V; scan rate, 900 mV/s; pulse amplitude, 75 mV; and SW frequency, 90 Hz. Adsorptive voltammetric peak current showed a linear response for Allura Red in the concentration range of 2.5 × 10−8 to 2.0 × 10−7 mol/L (r = 0.998). The limit of detection was 8.5 × 10−9 mol/L (4.2 ng/mL), the precision in terms of relative standard deviation was 1.3%, and the mean recovery was 102%. Possible interferences by several substances usually present in food products such as food additive azo dyes (E110, E102), gelatin, natural and artificial sweeteners, preservatives, and antioxidants were also evaluated. The proposed electrochemical procedure was successfully applied to the determination of this food dye in commercially available candy and a soft drink. The results were compared by statistical evaluation with those obtained by a reference spectrophotometric method.

Square-wave adsorptive stripping voltammetric determination of an antihistamine drug astemizole

2008 ◽  
Vol 62 (4) ◽  
Author(s):  
Ahmad Alghamdi
Keyword(s):  
Biological Fluids ◽  
Supporting Electrolyte ◽  
Reduction Process ◽  
High Sensitivity ◽  
Pulse Amplitude ◽  
Analytical Methodology ◽  
Square Wave ◽  
Pharmaceutical Tablets ◽  
Method Accuracy

AbstractThe square-wave voltammetric technique was used to explore the adsorption properties of the astemizole drug. The analytical methodology used was based on the adsorptive preconcentration of the drug on a hanging mercury drop electrode (HMDE), followed by the electrochemical reduction process which yielded a well-defined cathodic peak at −1.184 V (vs. the Ag/AgCl electrode). To achieve high sensitivity, various experimental and instrumental variables were investigated such as the supporting electrolyte, pH, accumulation time and potential, drug concentration, scan rate, SW frequency, pulse amplitude, convection rate, and the working electrode area. Under the optimized conditions, the AdSV peak current was proportional over the analyte concentration range of 5 × 10−7 to 2.5 × 10−6 mol L−1 (r = 0.998) with the detection limit of 1.4 × 10−8 mol L−1 (6.4 ng mL−1). The precision of the proposed method in terms of RSD was 2.4 %, whereas the method accuracy was indicated by the mean recovery of 100.1 %. Possible interferences of several substances usually present in the pharmaceutical tablets and formulations were also evaluated. The applicability of this electroanalytic approach was illustrated by the determination of astemizole in tablets and biological fluids.

Adsorptive Stripping Voltammetric Determination of Aluminium Using Arsenazo III

2001 ◽  
Vol 66 (3) ◽  
pp. 456-464 ◽  
Author(s):  
Alena Komersová ◽  
Martin Bartoš ◽  
Kurt Kalcher ◽  
Karel Vytřas
Keyword(s):  
Supporting Electrolyte ◽  
Pulse Amplitude ◽  
Arsenazo Iii ◽  
Pulse Interval ◽  
Divalent Metal Ions ◽  
Experimental Conditions ◽  
Accumulation Time ◽  
Indirect Determination ◽  
Calibration Dependence

A new analytical method for the indirect determination of trace amounts of aluminium(III) is based on the adsorptive accumulation of an azo dye, Arsenazo III, on the hanging mercury drop electrode. In the presence of Al(III) forming complexes with the dye, its peak occurring at -330 mV decreases. Optimized experimental conditions were found as follows: supporting electrolyte, acetate or chloroacetate-acetate buffer (pH 3.6); accumulation potential, -50 mV vs SCE; accumulation time, 120 s; Arsenazo III concentration, 5 · 10-7 mol l-1; scan rate, 20 mV s-1; initial potential, -500 mV; final potential, 0 mV; FSDPV mode; pulse amplitude, 50 mV; pulse duration, 100 ms; pulse interval, 10 ms. Under these experimental conditions, the calibration dependence was linear in the concentration range 0-6 μmol l-1 with a detection limit of 3.7 · 10-8 mol l-1 (1 ppb, calculated as 3σ for n = 10). Interference of iron(III) can be suppressed by addition of L-ascorbic acid, the effect of numerous divalent metal ions is negligible. Serious interfering effects were observed for U(VI) as UO22+, both Cr(III) and Cr(VI), and F-. The method developed was verified using a certified reference material (SRM 1575 Pine Needles); no statistically significant differences between the determined and declared contents were found.

scholarly journals Measurement of Voltammetric Peak Area and Resolution of Overlapping Peaks in the Simultaneous Determination of Copper, Lead, Cadmium, and Nickel in Environmental Matrix

2000 ◽  
Vol 83 (6) ◽  
pp. 1321-1326 ◽  
Author(s):  
Clinio Locatelli
Keyword(s):  
Simultaneous Determination ◽  
Analytical Procedure ◽  
Supporting Electrolyte ◽  
Differential Pulse ◽  
Standard Reference Materials ◽  
Experimental Conditions ◽  
Relative Standard ◽  
Resolution Of Overlapping Peaks ◽  
Determination Of Copper

Abstract Peak area was used for the simultaneous determination of copper(II), lead(II), cadmium(II), and nickel(II) in environmental matrixes by differential pulse voltammetry. The voltammetric measurements were performed with a conventional 3-electrode cell and an ammonia–ammonium chloride buffer, pH 9.1, as the supporting electrolyte. The analytical procedure was verified first in aqueous reference solutions and later by analysis of the standard reference materials Estuarine Sediment BCR-CRM 277 and River Sediment BCR-CRM 320. The precision and accuracy of the method, expressed as the relative standard deviation and the relative error, respectively, were <5% in all cases; the detection limit for each element under the experimental conditions used was in the range 1–5 μg/L. In the case of mutual interference between neighboring elements, an analytical procedure is proposed that is based on the standard additions technique, which allows the resolution of the overlapping voltammetric peaks.

scholarly journals An Electrochemiluminescence Sensor Based on Nafion/Magnetic Fe3O4 Nanocrystals Modified Electrode for the Determination of Bisphenol A in Environmental Water Samples

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2537 ◽  
Author(s):  
Jiye Chai ◽  
Xinru Yu ◽  
Jian Zhao ◽  
Aili Sun ◽  
Xizhi Shi ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Water Samples ◽  
Supporting Electrolyte ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Environmental Water ◽  
Experimental Conditions ◽  
Quenching Effect ◽  
Relative Standard

The well-dispersive and superparamagnetic Fe3O4-nanocrystals (Fe3O4-NCs) which could significantly enhance the anodic electrochemiluminescence (ECL) behavior of luminol, were synthesized in this study. Compared to ZnS, ZnSe, CdS and CdTe nanoparticles, the strongest anodic ECL signals were obtained at +1.6 V on the Fe3O4-NCs coated glassy carbon electrode. The ECL spectra revealed that the strong ECL resonance energy transfer occurred between luminol and Fe3O4-NCs. Furthermore, under the optimized ECL experimental conditions, such as the amount of Fe3O4-NCs, the concentration of luminol and the pH of supporting electrolyte, BPA exhibited a stronger distinct ECL quenching effect than its structural analogs and a highly selective and sensitive ECL sensor for the determination of bisphenol A (BPA) was developed based on the Fe3O4-NCs. A good linear relationship was found between the ECL intensity and the increased BPA concentration within 0.01–5.0 mg/L, with a correlation coefficient of 0.9972. The detection limit was 0.66 × 10−3 mg/L. Good recoveries between 96.0% and 105.0% with a relative standard deviation of less than 4.8% were obtained in real water samples. The proposed ECL sensor can be successfully employed to BPA detection in environmental aqueous samples.

Adsorptive cathodic stripping voltammetric determination of curcumin in turmeric and human serum

2011 ◽  
Vol 76 (3) ◽  
pp. 143-157 ◽  
Author(s):  
Mohammad Bagher Gholivand ◽  
Farhad Ahmadi ◽  
Alireza Pourhossein
Keyword(s):  
Human Serum ◽  
High Performance ◽  
Supporting Electrolyte ◽  
Cathodic Peak ◽  
Experimental Conditions ◽  
Accumulation Time ◽  
Accumulation Potential ◽  
Relative Standard ◽  
Cathodic Stripping

A simple, rapid, reliable and fully validated differential pulse adsorptive cathodic stripping voltammetric procedure has been developed for determination of the curcumin in human serum and turmeric, based on its electrochemical reduction at a hanging mercury drop electrode. The Britton–Robinson (BR) buffer of pH 9.5 was found to be reasonable as a supporting electrolyte for the assay of the compound. The effect of different parameters, such as pH, accumulation potential and accumulation time, on the sensitivity of method was evaluated. Under the optimized conditions (accumulation potential –0.3 V, accumulation time 50 s, BR buffer pH 9.5), curcumin was generated one irreversible cathodic peak. This cathodic peak showed a linear dependence on the concentration of curcumin over the range of 5.0 × 10–9–2.8 × 10–7 mol l–1. The obtained detection limit under the optimal experimental conditions is 1.5 × 10–9 mol l–1 after 50 s of the accumulation time. The relative standard deviation of 1.12% for concentration of 5.0 × 10–8 mol l–1 with 50 s accumulation time was obtained. The procedure was used successfully to the assay of the curcumin in turmeric and spiked human serum, and a good agreement was obtained between the results of the proposed method and high performance liquid chromatography (HPLC) analysis.

scholarly journals APPLICATION OF THE BISMUTH FILM ELECTRODE FOR THE DETERMINATION OF SELENIUM IN VITAMINIC COMPLEX BY SQUARE WAVE VOLTAMMETRY

2010 ◽  
Vol 07 (13) ◽  
pp. 6-15
Author(s):  
Joyce Nunes BIANCHIN ◽  
Marcel Silveira dos SANTOS ◽  
Almir SPINELLI
Keyword(s):  
Square Wave Voltammetry ◽  
Limit Of Detection ◽  
Copper Electrode ◽  
Analytical Sensitivity ◽  
Bismuth Film ◽  
Analytical Methodology ◽  
Square Wave ◽  
Wave Voltammetry ◽  
Relative Standard

This work was developed an analytical methodology for determination of selenium in samples of vitaminic complex using the square wave voltammetry (SWV). The analysis were performed with a copper electrode modified with bismuth film deposited by applying a potential of -400 mV for 60 seconds in a solution containing HCl 0.2 mol L-1, 20 g L-1 and 500 µg L-1 of Bi(III). The variables: time and conditioning potential were optimized by SWV for the formation of the bismuth film, after that, the variables that affect the analytical sensitivity for the reduction of Se (IV): frequency, increment and high of pulse were also optimized. The linearity of the method ranged from 90 – 1180 µg L-1. Limit of detection of 26,4 µg L-1, quantification limit of 87,9 µg L-1. Analytical frequency of 30 samples/hour. The method showed excellent precision, calculated as the relative standard deviation (RSD%) (n = 5) using spiked solution of 150 µg L-1. The concentration of selenium found in the sample of the vitaminic complex was 24 µg/tablet. A study of recovery was performed resulting in 92%.

scholarly journals Square-wave voltammetric determination of drospirenone and ethinylestradiol in pharmaceutical dosage form using square wave technique

2019 ◽  
Vol 10 (4) ◽  
pp. 305-316
Author(s):  
Ibrahim Hassan Habib ◽  
Mohammed Salem Rizk ◽  
Maha Sultan ◽  
Dalia Mohamed ◽  
Rehab Moussa Tony
Keyword(s):  
Supporting Electrolyte ◽  
Reference Method ◽  
Pulse Amplitude ◽  
Voltammetric Determination ◽  
Pharmaceutical Dosage Form ◽  
Square Wave ◽  
Significant Difference ◽  
Scan Rate ◽  
Wave Technique

Cathodic voltammetric behaviors of drospirenone and ethinylestradiol were used for the simultaneous determination of both drugs in bulk and in pharmaceutical formulation (Yasmin® tablets) without the interference of excipients. The determinations were made on hanging mercury dropping electrode using square-wave technique in a voltammetric cell containing 10 mL of 0.04 mole/L Britton-Robinson. After every aliquot addition, the solution was stirred for 10 s at 1000 rpm, rested for 10 s then square wave voltammetry mode was ramped from +100 to -1700 mV with scan rate of 100 mV/s, pulse amplitude of 50 mV and measurement time of 5 ms. Several factors such as pH, type of supporting electrolyte, pulse amplitude and scan rate were studied to optimize the condition for voltammetric determination of these drugs. With optimized experimental parameters, a good linearity was obtained for both drugs over a range of 1.36×10-6 to 1.91×10-7 mole/L and 6.75×10-8 to 6.07×10-7 mol/L of drospirenone and ethinylestradiol, respectively. Characterization of the proposed method was done according to International Conference on Harmonization, Q2B: Validation of Analytical procedures. The proposed method was statistically compared with the reference method and the results revealed no significant difference regarding accuracy and precision.

scholarly journals Determination of Carmine Food Dye (E120) in Foodstuffs by Stripping Voltammetry

2009 ◽  
Vol 92 (5) ◽  
pp. 1454-1459 ◽  
Author(s):  
Ahmad H Alghamdi ◽  
Hamed M Alshammery ◽  
Mohamed A Abdalla ◽  
Ali F Alghamdi
Keyword(s):  
Supporting Electrolyte ◽  
Reduction Process ◽  
Stripping Voltammetry ◽  
Pulse Amplitude ◽  
Acetate Buffer ◽  
Experimental Conditions ◽  
Accumulation Time ◽  
Food Dye ◽  
Voltammetric Technique

Abstract The behavior of the food colorant agent carmine (E120) was studied by square-wave adsorptive stripping voltammetry (SW-AdSV) at the hanging mercury drop electrode. It was observed that carmine gave a sensitive stripping voltammetric peak at 350 mV in pH 3 acetate buffer. The cyclic voltammetric technique was also used to characterize the electrochemical reduction process of carmine. The adsorptive voltammetric signal was evaluated with respect to various experimental conditions, and the optimized values were supporting electrolyte, acetate buffer; buffer acidity, pH 3; dye concentration, 3 107 M; accumulation time, 150 s; accumulation potential, 0.2 V; scan rate, 300 mV/s; pulse amplitude, 185 mV; SW frequency, 20 Hz; working electrode area, 0.6 mm2; and convection rate, 2600 rpm. The SW-AdSV peak currents depended linearly on the concentration of carmine from 5 108 to 1.25 107 mol/L (r 0.99). A detection limit of 1.43 109 mol/L with an RSD of 2.2 and a mean recovery of 97.9 were obtained. Possible interferences by several substances usually present in food products such as food additive dyes (E102, E100, E123, E127, and E129), artificial sweeteners, preservatives, and antioxidants were also evaluated. The proposed electrochemical procedure was successfully applied to the determination of carmine food dye in spiked commercially available ice cream and soft drinks.

Sign in / Sign up

Export Citation Format

Share Document