Square-wave voltammetric determination of drospirenone and ethinylestradiol in pharmaceutical dosage form using square wave technique

Cathodic voltammetric behaviors of drospirenone and ethinylestradiol were used for the simultaneous determination of both drugs in bulk and in pharmaceutical formulation (Yasmin® tablets) without the interference of excipients. The determinations were made on hanging mercury dropping electrode using square-wave technique in a voltammetric cell containing 10 mL of 0.04 mole/L Britton-Robinson. After every aliquot addition, the solution was stirred for 10 s at 1000 rpm, rested for 10 s then square wave voltammetry mode was ramped from +100 to -1700 mV with scan rate of 100 mV/s, pulse amplitude of 50 mV and measurement time of 5 ms. Several factors such as pH, type of supporting electrolyte, pulse amplitude and scan rate were studied to optimize the condition for voltammetric determination of these drugs. With optimized experimental parameters, a good linearity was obtained for both drugs over a range of 1.36×10-6 to 1.91×10-7 mole/L and 6.75×10-8 to 6.07×10-7 mol/L of drospirenone and ethinylestradiol, respectively. Characterization of the proposed method was done according to International Conference on Harmonization, Q2B: Validation of Analytical procedures. The proposed method was statistically compared with the reference method and the results revealed no significant difference regarding accuracy and precision.

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A Square-Wave Adsorptive Stripping Voltammetric Method for Determination of Fast Green Dye

Journal of AOAC International ◽

10.1093/jaoac/92.6.1714 ◽

2009 ◽

Vol 92 (6) ◽

pp. 1714-1719

Author(s):

Ali F Al-Ghamdi

Keyword(s):

Soft Drink ◽

Supporting Electrolyte ◽

Pulse Amplitude ◽

Fast Green ◽

Experimental Conditions ◽

Analytical Methodology ◽

Square Wave ◽

Carbonate Buffer ◽

Enhanced Sensitivity

Abstract Square-wave adsorptive stripping voltammetric (SW-AdSV) determinations of trace concentrations of the coloring agent fast green were described. The analytical methodology used was based on the adsorptive preconcentration of the dye on the hanging mercury drop electrode, and then a negative sweep was initiated. In pH 10 carbonate supporting electrolyte, fast green gave a well-defined and sensitive SW-AdSV peak at 1220 mV. The electroanalytical determination of this dye was found to be optimized in carbonate buffer (pH 10) with the following experimental conditions: accumulation time (120 s); accumulation potential (0.8 V); scan rate (800 mV/s); pulse amplitude (90 mV); frequency (90 Hz); surface area of the working electrode (0.6 mm2); and the convection rate (2000 rpm). Under these optimized conditions, the AdSV peak current was proportional over the concentration range 2 1086 107 M (r = 0.999), with an LOD of 1.63 1010 M (0.132 ppb). This analytical approach possessed more enhanced sensitivity than conventional chromatography or spectrophotometry, and was simple and quick. The precision of the method in terms of RSD was 0.17, whereas the accuracy was evaluated via the mean recovery of 99.6. Possible interferences by several substances usually present as food additive azo dyes (E110, E102, E123, and E129), natural and artificial sweeteners, and antioxidants were also investigated. Applicability of the developed electroanalysis method was illustrated via the determination of fast green in ice cream and soft drink samples.

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Square-wave adsorptive stripping voltammetric determination of an antihistamine drug astemizole

Chemical Papers ◽

10.2478/s11696-008-0041-z ◽

2008 ◽

Vol 62 (4) ◽

Cited By ~ 4

Author(s):

Ahmad Alghamdi

Keyword(s):

Biological Fluids ◽

Supporting Electrolyte ◽

Reduction Process ◽

High Sensitivity ◽

Pulse Amplitude ◽

Analytical Methodology ◽

Square Wave ◽

Pharmaceutical Tablets ◽

Method Accuracy

AbstractThe square-wave voltammetric technique was used to explore the adsorption properties of the astemizole drug. The analytical methodology used was based on the adsorptive preconcentration of the drug on a hanging mercury drop electrode (HMDE), followed by the electrochemical reduction process which yielded a well-defined cathodic peak at −1.184 V (vs. the Ag/AgCl electrode). To achieve high sensitivity, various experimental and instrumental variables were investigated such as the supporting electrolyte, pH, accumulation time and potential, drug concentration, scan rate, SW frequency, pulse amplitude, convection rate, and the working electrode area. Under the optimized conditions, the AdSV peak current was proportional over the analyte concentration range of 5 × 10−7 to 2.5 × 10−6 mol L−1 (r = 0.998) with the detection limit of 1.4 × 10−8 mol L−1 (6.4 ng mL−1). The precision of the proposed method in terms of RSD was 2.4 %, whereas the method accuracy was indicated by the mean recovery of 100.1 %. Possible interferences of several substances usually present in the pharmaceutical tablets and formulations were also evaluated. The applicability of this electroanalytic approach was illustrated by the determination of astemizole in tablets and biological fluids.

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A Square-Wave Adsorptive Stripping Voltammetric Method for the Determination of Amaranth, a Food Additive Dye

Journal of AOAC International ◽

10.1093/jaoac/88.3.788 ◽

2005 ◽

Vol 88 (3) ◽

pp. 788-793 ◽

Cited By ~ 6

Author(s):

Ahmad H Alghamdi

Keyword(s):

Soft Drink ◽

Supporting Electrolyte ◽

Pulse Amplitude ◽

Food Additive ◽

Experimental Conditions ◽

Analytical Methodology ◽

Square Wave ◽

Carbonate Buffer ◽

Relative Standard

Abstract Square-wave adsorptive stripping voltammetric (AdSV) determinations of trace concentrations of the azo coloring agent Amaranth are described. The analytical methodology used was based on the adsorptive preconcentration of the dye on the hanging mercury drop electrode, followed by initiation of a negative sweep. In a pH 10 carbonate supporting electrolyte, Amaranth gave a well-defined and sensitive AdSV peak at −518 mV. The electroanalytical determination of this azo dye was found to be optimal in carbonate buffer (pH 10) under the following experimental conditions: accumulation time, 120 s; accumulation potential, 0.0 V; scan rate, 600 mV/s; pulse amplitude, 90 mV; and frequency, 50 Hz. Under these optimized conditions the AdSV peak current was proportional over the concentration range 1 × 10−8 − 1.1 × 10−7 mol/L (r = 0.999) with a detection limit of 1.7 × 10−9 mol/L (1.03 ppb). This analytical approach possessed enhanced sensitivity, compared with conventional liquid chromatography or spectrophotometry and it was simple and fast. The precision of the method, expressed as the relative standard deviation, was 0.23%, whereas the accuracy, expressed as the mean recovery, was 104%. Possible interferences by several substances usually present as food additive azo dyes (E110, E102), gelatin, natural and artificial sweeteners, preservatives, and antioxidants were also investigated. The developed electroanalyticals method was applied to the determination of Amaranth in soft drink samples, and the results were compared with those obtained by a reference spectrophotometric method. Statistical analysis (paired t-test) of these data showed that the results of the 2 methods compared favorably.

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Simultaneous Square-Wave Voltammetric Determination of Paracetamol, Caffeine and Orphenadrine in Pharmaceutical Formulations Using a Cathodically Pretreated Boron-Doped Diamond Electrode

Electroanalysis ◽

10.1002/elan.201300137 ◽

2013 ◽

Vol 25 (7) ◽

pp. 1734-1741 ◽

Cited By ~ 48

Author(s):

Ana Paula Pires Eisele ◽

Débora Nóbile Clausen ◽

César Ricardo Teixeira Tarley ◽

Luiz Henrique Dall'Antonia ◽

Elen Romão Sartori

Keyword(s):

Pharmaceutical Formulations ◽

Voltammetric Determination ◽

Boron Doped Diamond ◽

Square Wave ◽

Diamond Electrode ◽

Boron Doped ◽

Boron Doped Diamond Electrode

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The effect of the supporting electrolyte on the voltammetric determination of the veterinary drug nitroxinil

Journal of Electroanalytical Chemistry ◽

10.1016/j.jelechem.2018.09.006 ◽

2018 ◽

Vol 827 ◽

pp. 21-26 ◽

Cited By ~ 3

Author(s):

Karolina Sipa ◽

Mariola Brycht ◽

Sławomira Skrzypek

Keyword(s):

Supporting Electrolyte ◽

Voltammetric Determination ◽

Veterinary Drug

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Square-wave voltammetric determination of pantoprazole using ex situ plated antimony-film electrode

Electrochimica Acta ◽

10.1016/j.electacta.2013.07.173 ◽

2013 ◽

Vol 109 ◽

pp. 818-822 ◽

Cited By ~ 19

Author(s):

Biljana Nigović ◽

Samo B. Hocevar

Keyword(s):

Voltammetric Determination ◽

Ex Situ ◽

Film Electrode ◽

Square Wave ◽

Antimony Film

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HemoCue against Coulter LH-750 in the estimation of hemoglobin levels of blood donors in mobile collection settings: a comparative study

Medical Journal of Indonesia ◽

10.13181/mji.v27i4.2635 ◽

2018 ◽

Vol 27 (4) ◽

pp. 250-5

Author(s):

Sultan A.M. Saghir ◽

Amer A. Almaiman ◽

Aishah K.A. Shatar ◽

Norris Naim ◽

Huda S. Baqir

Keyword(s):

Blood Donors ◽

Reference Method ◽

Cross Sectional Study ◽

Outpatient Setting ◽

Concordance Correlation ◽

Cross Sectional ◽

New Device ◽

Coefficients Of Variation ◽

Significant Difference

Background: The fast and outpatient setting for a determination of the hemoglobin (Hb) level is a well-recognized prerequisite to detect anemia in blood donors. This study aimed to evaluate the performance of the HemoCue methods (HemoCue B-Hb and HemoCue-301) against Coulter LH-750 as a reference method for Hb determination.Methods: This study was an experimental cross-sectional study. It includes 455 blood samples that were collected from volunteer blood donors between January 15, 2010 and February 15, 2011. The performance of the three methods and their comparisons were assessed using the analysis of coefficients of variation (CV), linear regression, and mean difference. Correlation coefficient and Bland–Altman plots were drawn to compare the two HemoCue measurements and the automated cell analyzer against each other and to evaluate their results. The Hb concentrations were compared using the concordance correlation coefficient.Results: The findings exhibited that the CV for the three methods Coulter LH-750, HemoCue B-Hb, and HemoCue-301 were 0.60%, 0.72%, and 0.92%, respectively. A statistically significant difference was observed between the means of the Hb measurements for the three methods (p<0.001). The HemoCue B-Hb and HemoCue-301 methods showed the best agreement, and the Coulter LH-750 method gave a lower Hb value compared with the two HemoCue methods. The results showed a positive correlation of HemoCue Hb results compared with the reference method.Conclusion: All three methods provide a good agreement for Hb determination. The new device HemoCue-301 was found to be more accurate compared with HemoCue B-Hb and Coulter LH-750.

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