scholarly journals Determination of Chloramphenicol Residue in Chicken Tissues by Immunoaffinity Chromatography Cleanup and Gas Chromatography with aMicrocell Electron Capture Detector

2006 ◽  
Vol 89 (2) ◽  
pp. 369-373 ◽  
Author(s):  
Suxia Zhang ◽  
Jinhui Zhou ◽  
Jianzhong Shen ◽  
Shuangyang Ding ◽  
Jiancheng Li
Keyword(s):  
Gas Chromatography ◽  
Electron Capture ◽  
Chicken Liver ◽  
Electron Capture Detector ◽  
Immunoaffinity Column ◽  
Chicken Tissues ◽  
Chicken Muscle ◽  
Limit Of Quantitation ◽  
Sepharose 4B

Abstract A rapid and sensitive gas chromatography (GC) method was developed to detect chloramphenicol in chicken tissues. The extracted samples were cleaned up using the immunoaffinity column prepared by coupling antichloramphenicol monoclonal antibody with cyanogen bromide-activated Sepharose 4B. The dynamic column capacity of chloramphenicol was 3265 ng/mL gel. The eluate was evaporated to dryness, and residues were derivatized and determined by GC with a microcell electron capture detector. Average recoveries were 86.6 to 96.9 for chicken muscle and 74.3 to 96.1 for chicken liver. The limit of quantitation of the method was 0.05 ng/g for chicken muscle and 0.1 ng/g for chicken liver.

Gas Chromatographic Determination of N-Butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine and α,α,α-Trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine in Formulations

1971 ◽  
Vol 54 (3) ◽  
pp. 711-712
Author(s):  
Martha Fuzesi
Keyword(s):  
Gas Chromatography ◽  
Quantitative Determination ◽  
Electron Capture ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Electron Capture Detector ◽  
Reference Solution ◽  
Gas Chromatographic Method

Abstract A gas chromatographic method is described for the quantitative determination of N-butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-tolindine and α,α,α-trifluoro-2,6-dinitro-N,N-dipropyI-p-toluidine herbicides in formulations. The sample is extracted with benzene, and equal amounts of sample and reference solution in the same concentration range are analyzed by gas chromatography, using an electron capture detector and an SE-30/Diatoport S column. The method has been applied successfully to laboratory-prepared and commercial samples.

Determination of indicator polychlorinated biphenyls (PCBs) by gas chromatography–electron capture detector

Chemosphere ◽  
2013 ◽  
Vol 93 (8) ◽  
pp. 1556-1560 ◽  
Author(s):  
Samuel Afful ◽  
Johannes A.M. Awudza ◽  
Stevester K. Twumasi ◽  
Shiloh Osae
Keyword(s):  
Gas Chromatography ◽  
Polychlorinated Biphenyls ◽  
Electron Capture ◽  
Electron Capture Detector

Determination of Monensin in Chicken Tissues by Liquid Chromatography with Postcolumn Derivatization

1994 ◽  
Vol 77 (4) ◽  
pp. 885-890 ◽  
Author(s):  
John W Moran ◽  
J Matthew Rodewald ◽  
Alvin L Donoho ◽  
Mark R Coleman
Keyword(s):  
Liquid Chromatography ◽  
Silica Gel ◽  
Chicken Tissues ◽  
Chicken Muscle ◽  
Limit Of Quantitation ◽  
Microbiological Techniques ◽  
Wavelength Detector ◽  
Acidic Conditions ◽  
Postcolumn Derivatization

Abstract A method is described for the detection and quantitation of monensin in chicken tissues by liquid chromatography with postcolumn derivatization with vanillin. Monensin is extracted from the tissues by homogenization with methanol–water and is isolated and concentrated by liquid–liquid partition and sorbent extraction with silica gel. Monensin is mixed postcolumn with vanillin under acidic conditions and heated, and the resulting products are measured by a variable-wavelength detector operating at 520 nm. The method has a limit of quantitation of 0.025 μg/g and is validated for use in the analyses of chicken muscle, liver, and skin (with adhering fat tissues) for monensin. Standard recoveries from the 3 tissue types tested at 3 levels ranged from 82 to 96%. The method represents an improvement in specificity, accuracy, and analysis time over existing methods, which use microbiological techniques.

Gas Chromatographic Determination of Pentachlorophenol in Gelatin

1984 ◽  
Vol 67 (2) ◽  
pp. 275-277
Author(s):  
Arnold P Borsetti ◽  
Lee S Thurston
Keyword(s):  
Gas Chromatography ◽  
Liquid Phase ◽  
Electron Capture ◽  
Limit Of Detection ◽  
Direct Determination ◽  
Chromatographic Determination ◽  
Gelatin Matrix ◽  
Limit Of Quantitation ◽  
Acetate Derivative

Abstract A method is described for the determination of pentachlorophenol (PCP) in gelatin. The method employs acid and heat to hydrolyze the gelatin matrix, a base partition and wash for separation and cleanup, and a reacidification and extraction with hexane for direct determination of PCP, without preparation of a derivative, using gas chromatography (GC) with a 1% SP-1240DA liquid phase and a 63Ni electron capture detector. Recoveries averaged 106% for fortifications between 0.02 and 1.0 ppm. The limit of quantitation is 20 ppb. The limit of detection is 4-6 ppb. The method, which has undergone a successful intralaboratory trial, is simple and rapid, and requires only general laboratory reagents and equipment. GC of the acetate derivative of PCP is used for confirmation of identity.

scholarly journals Simultaneous Determination of Florfenicol and Florfenicol Amine in Fish, Shrimp, and Swine Muscle by Gas Chromatography with a Microcell Electron Capture Detector

2006 ◽  
Vol 89 (5) ◽  
pp. 1437-1442 ◽  
Author(s):  
Suxia Zhang ◽  
Fengyun Sun ◽  
Jiancheng Li ◽  
Linli Cheng ◽  
Jianzhong Shen
Keyword(s):  
Gas Chromatography ◽  
Detection Limit ◽  
Simultaneous Determination ◽  
Electron Capture ◽  
Solid Phase ◽  
Electron Capture Detector ◽  
Phase Extraction ◽  
Chromatography Method ◽  
Residue Study

Abstract A rapid and sensitive gas chromatography method was developed for the simultaneous determination of florfenicol (FF) and its metabolite florfenicol amine (FFA) in fish, shrimp, and swine muscle. The extracted samples were defatted with hexane and cleaned up by solid-phase extraction using Oasis MCX cartridges. The eluate was evaporated to dryness, and residues were derivatized and determined by gas chromatography with a microcell electron capture detector. Overall average recoveries ranged from 81.7 to 109.7% for fish, 94.1 to 103.4% for shrimp, and 71.5 to 91.4% for swine muscle. The detection limit was 0.5 ng/g for FF and 1 ng/g for FFA, respectively. The method was validated for the determination of incurred swine muscle samples in an actual residue study.

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