scholarly journals Determination of New Antidepressants in Pharmaceuticals by Thin-Layer Chromatography with Densitometry

2010 ◽  
Vol 93 (3) ◽  
pp. 778-782 ◽  
Author(s):  
Tatána Gondová ◽  
Iveta Petríková
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Simultaneous Separation ◽  
Tlc Plates ◽  
Precision And Accuracy ◽  
Layer Chromatography

Abstract A new and simple TLC-densitometry method has been developed for the simultaneous separation of the two noradrenergic and specific serotonergic antidepressants mirtazapine and mianserine and validated for their determination in commercially available tablets. The method used TLC plates precoated with silica gel 60F254 as the stationary phase, and the mobile phase consisted of hexaneisopropanol25 ammonia (70 + 25 + 5, v/v/v). Densitometric analysis was carried out in the absorbance mode at 280 nm. The method was validated in accordance with International Conference on Harmonization guidelines in terms of linearity, LOD, LOQ, precision, and accuracy. Calibration curves were linear (R2 > 0.9970) with respect to peak area in the concentration range of 5002500 and 5005000 ng/spot for mirtazapine and mianserine, respectively. The LODs were 20 and 35 ng/spot for mirtazapine and mianserine, respectively. The described method was successfully applied to the determination of mirtazapine and mianserine in their pharmaceutical formulations with recovery ranging from 99.83 to 101.20 of the labeled amount of the compounds. The proposed method can be used in routine QC of these drugs in pharmaceutical formulations.

scholarly journals Determination of the Lipophilicity of Ibuprofen, Naproxen, Ketoprofen, and Flurbiprofen with Thin-Layer Chromatography

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Andrzej Czyrski
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Human Body ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Regression Curve ◽  
Layer Chromatography

The lipophilicity is an important parameter that influences the activity of the drugs in the human body. The reversed phase high performance thin layer chromatography was applied to determine the Log P values of ibuprofen, ketoprofen, naproxen, and flurbiprofen. The stationary phase used in the study was silica-gel coated plates. The mobile phase was the mixture of acetonitrile and water in different proportions. The content of acetonitrile varied in 5% increments from 50% to 80%. The Rm0 values were determined for the compounds with a known Log P and for the analyzed substances (ibuprofen, naproxen, ketoprofen, and flurbiprofen). The Log P values were calculated for the analyzed compounds using the regression curve Rm0 = f(Log P) parameters for the compounds with the known lipophilicity. Flurbiprofen is characterized by the highest Log P value: 3.82. The lowest one is noted for ketoprofen: 2.66. The determined Log P values of tested compounds were similar to the values calculated by the software.

Determination of poloxamer 188 and poloxamer 407 using high-performance thin-layer chromatography in pharmaceutical formulations

2008 ◽  
Vol 46 (4) ◽  
pp. 799-803 ◽  
Author(s):  
Z. Urbán-Morlán ◽  
R. Castro-Ríos ◽  
A. Chávez-Montes ◽  
L.M. Melgoza-Contreras ◽  
E. Piñón-Segundo ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Pharmaceutical Formulations ◽  
Poloxamer 407 ◽  
Poloxamer 188 ◽  
Layer Chromatography

scholarly journals Some Observations on Thin Layer Chromatography Technique

2019 ◽  
Vol 8 (2) ◽  
pp. 1700-1702
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Potassium Permanganate ◽  
Mobile Phase ◽  
Technical Aspects ◽  
Potassium Permanganate Solution ◽  
Tlc Plates ◽  
Solvent Use ◽  
Layer Chromatography

The present study is to report the various problems faced during TLC methodology. Although used regularly some technical aspects must be kept in mind to get better and uniform results. During our experiments with TLC methods we came across some problems and here these aspects of TLC methodology are being highlighted. It is suggested that the solvent use as mobile phase should also be used for extraction of any particular phytochemical. TLC plates should be 3 to 4 mm thick, to be dried for at least 72 hrs. It is also suggested that potassium permanganate solution gives better clarity in visualizing the spots

scholarly journals Determination of Cortisol in Human Plasma by Radioimmunoassay

1977 ◽  
Vol 14 (1-6) ◽  
pp. 343-349 ◽  
Author(s):  
G. F. Read ◽  
Diana R. Fahmy ◽  
R. F. Walker
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Plasma ◽  
Plasma Cortisol ◽  
Specific Radioimmunoassay ◽  
Precision And Accuracy ◽  
Layer Chromatography

A radioimmunoassay for plasma cortisol featuring the gamma-emitting radioligand 125I-iodohistamine, coupled to cortisol-3-(O-carboxymethyl)-oxime, is described. The new procedure retains much of the specificity associated with the use of anti-cortisol-3-BSA sera with tritium-labelled radioligands, and has the further advantages that running costs are lower and there is a greater potential for automation. Cortisol values obtained by this procedure agree well with those obtained by a published specific radioimmunoassay using the tritiated cortisol radioligand. Specificity of the procedure was checked by comparing values obtained with and without thin-layer chromatography purification: correlation was excellent (r = 0·96). Satisfactory levels of sensitivity, precision, and accuracy were obtained.

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