Theoretical determination of optimum composition of the binary mobile phase for distribution of substances in thin-layer chromatography

1981 ◽  
Vol 4 (5) ◽  
pp. 224-227 ◽  
Author(s):  
G. Chojnacka ◽  
J. K. Różyło ◽  
I. Malinowska
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Optimum Composition ◽  
Theoretical Determination ◽  
Binary Mobile Phase ◽  
Layer Chromatography

scholarly journals Determination of New Antidepressants in Pharmaceuticals by Thin-Layer Chromatography with Densitometry

2010 ◽  
Vol 93 (3) ◽  
pp. 778-782 ◽  
Author(s):  
Tatána Gondová ◽  
Iveta Petríková
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Simultaneous Separation ◽  
Tlc Plates ◽  
Precision And Accuracy ◽  
Layer Chromatography

Abstract A new and simple TLC-densitometry method has been developed for the simultaneous separation of the two noradrenergic and specific serotonergic antidepressants mirtazapine and mianserine and validated for their determination in commercially available tablets. The method used TLC plates precoated with silica gel 60F254 as the stationary phase, and the mobile phase consisted of hexaneisopropanol25 ammonia (70 + 25 + 5, v/v/v). Densitometric analysis was carried out in the absorbance mode at 280 nm. The method was validated in accordance with International Conference on Harmonization guidelines in terms of linearity, LOD, LOQ, precision, and accuracy. Calibration curves were linear (R2 > 0.9970) with respect to peak area in the concentration range of 5002500 and 5005000 ng/spot for mirtazapine and mianserine, respectively. The LODs were 20 and 35 ng/spot for mirtazapine and mianserine, respectively. The described method was successfully applied to the determination of mirtazapine and mianserine in their pharmaceutical formulations with recovery ranging from 99.83 to 101.20 of the labeled amount of the compounds. The proposed method can be used in routine QC of these drugs in pharmaceutical formulations.

scholarly journals Normal phase thin layer chromatography for aromatic derivatives of 3-chloro-1,4-naphtochinone

2021 ◽  
Vol 4 (1) ◽  
pp. 33-43
Author(s):  
O. Ya. Smirnova ◽  
◽  
Yo. Yo. Yatchyshyn ◽  
S. V. Kolobych ◽  
I. P. Poliuzhyn ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Normal Phase ◽  
Polar Component ◽  
Polar Solvents ◽  
Analyte Molecule ◽  
Binary Mobile Phase ◽  
Layer Chromatography ◽  
Derivatives Of

The chromatographic characteristics were investigated for eight aromatic derivatives of 3-chloro-1,4-naphthoquinone under conditions of normal-phase thin-layer chromatography for benzene-based binary mobile phase and such polar solvents as chloroform, acetone, acetonitrile, methanol and propan-2-ol. The slope of linear retention dependencies for the investigated compounds on the concentration of the polar component in the mobile phase satisfactorily correlates with the area occupied by the adsorbed analyte molecule in the stationary phase. The intercept in the Soczewinski equation depends on the polar component of the mobile phase.

Determination of 1,8-Cineole in Eucalyptus Essential Oils by Thin-Layer Chromatography and Densitometry

1995 ◽  
Vol 78 (1) ◽  
pp. 115-117 ◽  
Author(s):  
Carmen Rossini ◽  
Enrique Pandolfi ◽  
Eduardo Dellacassa ◽  
Patrick Moyna
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Light Petroleum ◽  
Gas Liquid Chromatography ◽  
Acid Reagent ◽  
Layer Chromatography

Abstract A thin-layer chromatography (TLC) method with scanning is described for evaluation of the 1,8-cineole content of Eucalyptus essential oils. Samples were developed on silica gel G 60 with light petroleum–chloroform (70 + 30) as mobile phase. Color was developed with 4-dimethylaminobenzal-dehyde–sulfuric acid reagent. The absorbance of colored spots was measured at 460 nm. The response was linear between 15 to 75 μg of 1,8-cineole, with a correlation coefficient of 0.9997. The results of each assay were compared with those from gas–liquid chromatography (GLC). The TLC to GLC ratio was constant (0.92).

scholarly journals Preliminary Studies on the Content of Phytochemical Compounds On Skin of Salak Fruit (Salacca zalacca)

2020 ◽  
Vol 6 (1) ◽  
pp. 1-6
Author(s):  
Rety Setyawaty ◽  
◽  
Ruli Aptuning B ◽  
Dewanto Dewanto ◽  
◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Economic Value ◽  
Phytochemical Analysis ◽  
Native Plant ◽  
Active Components ◽  
Phytochemical Compounds ◽  
Layer Chromatography

Salak (Salaccazalacca) is a native plant of Indonesia. Some Indonesians use zalacca as a traditional medicine. This plant belongs to the Palmae family. The purpose of this study is to reveal the structure of the active components that are efficacious and increase the economic value and efficacy that exist in the skin of zalacca (Salaccazalacca).The extraction method chosen was the maceration method using 96% ethanol solvent. A total of 1 kg of fresh zalacca skin (Salaccazalacca) is dried using the sun-heating method and produces 625 g of dried zalacca skin. The extraction-maceration process uses 100 grams of implicial. Simplisia is soaked and stirred occasionally in 96% ethanol for 3 days and filtered until a thick extract is obtained. Thick extract obtained was 10.69 g. The percentage of yield obtained was 10.69%. Phytochemical screening carried out is alkaloids, steroids, triterpenoids, saponins, tannins, and flavonoids by using chemical reactions and using thin layer chromatography. The mobile phase used in thin layer chromatography is toluene: ethyl acetate (9.3: 0.7). Determination of the mobile phase is done based on a trial and error approach to obtain a clear line of movement. The stationary phase uses silica gel GF 254.Based on phytochemical analysis, the skin of salak fruit (Salaccazalacca) contains alkaloids, triterpenoids, saponins, tannins, and flavonoids. In addition, based on analysis using thin layer chromatography it produces Rf 0.1875 and is thought to contain terpene alcohol.

Liquid Chromatographic Determination of Aspartame in Dry Beverage Bases and Sweetener Tablets with Confirmation by Thin Layer Chromatography

1984 ◽  
Vol 67 (3) ◽  
pp. 513-515
Author(s):  
Daniel H Daniels ◽  
Frank L Joe ◽  
Charles R Warner ◽  
Thomas Fazio
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Layer Chromatography

Abstract A liquid chromatographic method is described for the determination of aspartame in dry beverage bases and sweetener tablets. The sample was mixed with the mobile phase, the pH was adjusted to within ±0.1 pH unit of the mobile phase, and the sample was diluted to volume with the mobile phase. The solution was Altered and a 10 μL aliquot was injected onto a C18 reverse phase column. Aspartame was quantitated with an ultraviolet detector. Recoveries of aspartame ranged from 94 to 111%. The dry beverage bases contained 5–13% aspartame and the sweetener tablets contained 19% aspartame. The presence of aspartame was confirmed by using thin layer chromatography.

Determination of Cimetidine, Famotidine, and Ranitidine Hydrochloride in the Presence of Their Sulfoxide Derivatives in Pure and Dosage Forms by High-Performance Thin-Layer Chromatography and Scanning Densitometry

2002 ◽  
Vol 85 (5) ◽  
pp. 1015-1020 ◽  
Author(s):  
Khadiga M Kelani ◽  
Azza M Aziz ◽  
Maha A Hegazy ◽  
Laila Abdel Fattah
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Accurate Method ◽  
Dosage Forms ◽  
Ranitidine Hydrochloride ◽  
Layer Chromatography

Abstract A selective, precise, and accurate method was developed for the determination of cimetidine (C), famotidine (F), and ranitidine hydrochloride (R·HCI)in the presence of their sulfoxide derivatives. The method involves quantitative densitometric evaluation of mixtures of the drugs and their derivatives after separation by high-performance thin-layer chromatography on silica gel plates (10 × 20 cm) with ethyl acetate–isopropanol–20% ammonia (9 + 5 + 4, v/v) as the mobile phase for both C and F and ethyl acetate–methanol–20% ammonia (10 + 2 + 2, v/v) as the mobile phase for R·HCI; Rf values for C, F, and R·HCI and their corresponding derivatives were 0.85 and 0.59, 0.73 and 0.41, and 0.56 and 0.33, respectively. Developing time was approximately 20 min. For densitometric evaluation, peak areas were recorded at 218, 265, and 313 nm for C, F, and R·HCI, respectively. The relationship between concentration and the corresponding peak area was plotted for the ranges of 5–50 μg/spot for C and 2–20 μg/spot for F and R·HCl. Mean recoveries were 100.39 ± 1.33, 99.77 ± 1.30, and 100.09 ± 0.69% for C, F, and R·HCI, respectively. The proposed method was used successfully for stability testing of the pure drugs in the presence of up to 90% of their degradates, in bulk powder and dosage forms. The results obtained were analyzed statistically and compared with those obtained by the official methods.

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