scholarly journals AOAC SMPR 2010.002: Standard Method Performance Requirements for Polymerase Chain Reaction (PCR) Methods for Detection of Yersinia pestis in Aerosol Collection Filters and/or Liquids

2011 ◽  
Vol 94 (4) ◽  
pp. 1342-1346 ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Yersinia Pestis ◽  
Standard Method ◽  
Chain Reaction ◽  
Method Performance ◽  
Performance Requirements ◽  
Polymerase Chain

Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia

2016 ◽  
Vol 47 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Stanley S Nyirenda ◽  
Bernard M Hang’ombe ◽  
Bukheti S Kilonzo ◽  
Mathews N Kabeta ◽  
Mundia Cornellius ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Yersinia Pestis ◽  
Plasminogen Activator ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Recent Past ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Pcr Techniques ◽  
Suspected Outbreak

Plague is a re-emerging zoonotic disease caused by the bacterium Yersinia pestis. The disease has caused periodic global devastation since the first outbreak in the 6th century. Two months after a suspected plague outbreak in Nyimba district, samples were collected from 94 livestock (goats and pigs), 25 rodents, 6 shrews and 33 fleas. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) techniques were used to investigate the presence of Y. pestis, which showed that 16.0% (4/25) of rodents, 16.7% (1/6) of shrews ( Crocidura spp) and 6.0% (5/83) of goats were positive for IgG antibodies against Fraction 1 antigen of Y. pestis. Plasminogen activator (Pla) gene (DNA) of Y. pestis was detected in five pools containing 36.4% (12/33) fleas collected from pigs (n = 4), goats (n = 5) and rodents (n = 3). The detection of Pla gene in fleas and IgG antibodies against Fraction1 antigen in rodents, shrews and goats suggest that Y. pestis had been present in the study area in the recent past.

Polymerase Chain Reaction Assays for the Presumptive Identification of Yersinia pestis Strains in Georgia

2006 ◽  
pp. 333-336 ◽  
Author(s):  
Lela Bakanidze ◽  
Ioseb Velijanashvili ◽  
Merab Kekelidze ◽  
Levan Beridze ◽  
Ekaterine Zangaladze ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Yersinia Pestis ◽  
Chain Reaction ◽  
Presumptive Identification ◽  
Polymerase Chain

scholarly journals A Real-Time Fluorescence Polymerase Chain Reaction Assay for the Identification of Yersinia pestis Using a Field-Deployable Thermocycler

2003 ◽  
Vol 168 (10) ◽  
pp. 852-855 ◽  
Author(s):  
James C. McAvin ◽  
Mariana A. McConathy ◽  
Andrew J. Rohrer ◽  
William B. Huff ◽  
William J. Barnes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Yersinia Pestis ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Chain Reaction ◽  
Polymerase Chain

Confirmation of Presumptive Salmonella Colonies by the Polymerase Chain Reaction

1998 ◽  
Vol 61 (10) ◽  
pp. 1381-1383 ◽  
Author(s):  
A. ŠTEFANOVIČOVÁ ◽  
H. REHÁKOVÁ ◽  
A. ŠKARKOVÁ ◽  
N. RIJPENS ◽  
T. KUCHTA
Keyword(s):  
Polymerase Chain Reaction ◽  
Standard Method ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Serological Identification ◽  
Pcr Method ◽  
Selective Plating ◽  
Polymerase Chain ◽  
Time Required

The potential of a genus-specific polymerase chain reaction (PCR) for the confirmation of Salmonella colonies was evaluated on 209 presumptive Salmonella colonies obtained by the standard method ISO 6579. The PCR method employing primers STII and ST15 (S. Aabo et al., Mol. Cell. Probes 7:171–178, 1993) gave results identical (100%) to those of the biochemical and serological identification, in terms of discrimination of Salmonella from non-Salmonella strains. PCR could be used directly on the colonies from selective plating media, which allowed a reduction of the time required for confirmation to a maximum of 6 h.

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