Immortalized Dendritic Cell Line with Efficient Cross-Priming Ability Established from Transgenic Mice Harboring the Temperature-Sensitive SV40 Large T-Antigen Gene

2004 ◽  
Vol 136 (3) ◽  
pp. 321-328 ◽  
Author(s):  
S. Ebihara
Keyword(s):  
Dendritic Cell ◽  
Transgenic Mice ◽  
Cell Line ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Antigen Gene

Establishment of a novel chondrocyte-like cell line derived from transgenic mice harboring the temperature-sensitive simian virus 40 large T-antigen gene

2009 ◽  
Vol 11 (11) ◽  
pp. 1646-1654 ◽  
Author(s):  
Nobuko Mataga ◽  
Masato Tamura ◽  
Nobuyuki Yanai ◽  
Tamayuki Shinomura ◽  
Koji Kimata ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Line ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Antigen Gene

Cisplatin-induced toxicity in immortalized renal cell lines established from transgenic mice harboring temperature sensitive SV40 large T-antigen gene

1996 ◽  
Vol 70 (5) ◽  
pp. 284-292 ◽  
Author(s):  
Makoto Hosoyamada ◽  
Masuo Obinata ◽  
Misao Suzuki ◽  
H. Endou
Keyword(s):  
Transgenic Mice ◽  
Cell Lines ◽  
Renal Cell ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Antigen Gene ◽  
Renal Cell Lines

scholarly journals A Tubule Cell Line Established from Transgenic Mice Harboring Temperature-sensitive Simian Virus 40 Large T-Antigen Gene

1991 ◽  
Vol 82 (12) ◽  
pp. 1344-1348 ◽  
Author(s):  
Nobuaki Yanai ◽  
Takeshi Satoh ◽  
Shiro Kyo ◽  
Keishi Abe ◽  
Misao Suzuki ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Line ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Tubule Cell ◽  
Antigen Gene

ESTABLISHMENT OF LEYDIG CELL LINE, TTE1, FROM TRANSGENIC MICE HARBORING TEMPERATURE-SENSITIVE SIMIAN VIRUS 40 LARGE T-ANTIGEN GENE

2002 ◽  
Vol 48 (1) ◽  
pp. 43-51 ◽  
Author(s):  
S. Ohta ◽  
Y. Tabuchi ◽  
N. Yanai ◽  
S. Asano ◽  
H. Fuse ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Line ◽  
Leydig Cell ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Antigen Gene

scholarly journals Paracrine effects of transplanted mesothelial cells isolated from temperature-sensitive SV40 large T-antigen gene transgenic rats during peritoneal repair

2013 ◽  
Vol 29 (2) ◽  
pp. 289-300 ◽  
Author(s):  
R. Kanda ◽  
C. Hamada ◽  
K. Kaneko ◽  
T. Nakano ◽  
K. Wakabayashi ◽  
...  
Keyword(s):  
Mesothelial Cells ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Large T Antigen ◽  
Transgenic Rats ◽  
Sv40 Large T Antigen ◽  
Paracrine Effects ◽  
Antigen Gene

Growth Properties of Neural Cell Lines Immortalized with the Tsa58 Allele of Sv40 Large T Antigen

1997 ◽  
Vol 6 (3) ◽  
pp. 231-238 ◽  
Author(s):  
M.E. Truckenmiller ◽  
Ora Dillon-Carter ◽  
Carlo Tornatore ◽  
Henrietta Kulaga ◽  
Hidetoshi Takashima ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cell Line ◽  
Cell Lines ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Large T Antigen ◽  
Wild Type ◽  
Sv40 Large T Antigen ◽  
Growth Properties

In vitro growth properties of three CNS-derived cell lines were compared under a variety of culture conditions. The M213-20 and J30a cell lines were each derived from embryonic CNS culture with the temperature-sensitive (ts) allele of SV40 large T antigen, tsA58, while the A7 cell line was immortalized using wild-type SV40 large T antigen. Cells immortalized with tsA58 SV40 large T proliferate at the permissive temperature, 33° C, while growth is expected to be suppressed at the nonpermissive temperature, 39.5°C. Both the M213-20 and J30a cell lines were capable of proliferating at 39.5°C continuously for up to 6 mo. All three cell lines showed no appreciable differences in growth rates related to temperature over a 7-day period in either serum-containing or defined serum-free media. The percentage of cells in S-phase of the cell cycle did not decrease or was elevated at 39.5°C for all three cell lines. After 3 wk at 39.5°C, the three cell lines also showed positive immunostaining using two monoclonal antibodies reacting with different epitopes of SV40 large T antigen. Double strand DNA sequence analyses of a 300 base pair (bp) fragment of the large T gene from each cell line, which included the ts locus, revealed mutations in both the J30a and M213-20 cell lines. The J30a cell line ts mutation had reverted to wild type, and two additional loci with bp substitutions with predicted amino acid changes were also found. While the ts mutation of the M213-20 cells was retained, an additional bp substitution with a predicted amino acid change was found. The A7 cell line sequence was identical to the reference wild-type sequence. These findings suggest that (a) nucleic acid sequences in the temperature-sensitive region of the tsA58 allele of SV40 large T are not necessarily stable, and (b) temperature sensitivity of cell lines immortalized with tsA58 is not necessarily retained.

scholarly journals Agonists stimulated cAMP response in renal cell lines established from transgenic mice harboring SV40 large T antigen gene.

1994 ◽  
Vol 64 ◽  
pp. 177
Author(s):  
Makoto Hosoyamada ◽  
Michio Takeda ◽  
Akiko Mizuno ◽  
Takashi Sekine ◽  
Hitoshi Endou
Keyword(s):  
Transgenic Mice ◽  
Cell Lines ◽  
Renal Cell ◽  
T Antigen ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Antigen Gene ◽  
Renal Cell Lines
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