Agonists stimulated cAMP response in renal cell lines established from transgenic mice harboring SV40 large T antigen gene.

In the mouse and human, mRNA transcripts encoding the lymphocyte-specific protein tyrosine kinase p56lck are derived from two separate promoters resulting in heterogeneity in the 5' untranslated region sequence. The proximal promoter lies just 5' to the coding region for the gene and is active only in thymocytes. In contrast, the distal promoter lies 34 kilobases (kb) 5' in the human, and is active both in thymocytes and mature peripheral T cells. As previously reported, transgenic mice bearing functional proximal promoter sequence juxtaposed with the SV40 large T antigen gene invariably develop lymphoid tumors confined to the thymus. In the current work, transgenic mice bearing a 2.6-kb fragment of the human distal promoter fused to the SV40 large T antigen gene express large T antigen in thymocytes and in peripheral lymphoid cells, and develop tumors of both the thymus and the peripheral lymphoid organs. The ability of the human distal promoter to function appropriately in transgenic mice is consistent with the strong similarity observed between the mouse and human distal promoter sequences. With the exception of a single short interval that serves as a target for binding of nuclear factors, significant sequence similarity is not seen when the distal and proximal promoter sequences are compared. Hence, developmentally regulated, lineage-specific transcription of the lck gene is mediated by distinct promoter sequences that appear to be capable of functioning independently.

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Establishment of early proximal tubule (Si) cell line selectively expressing V1a receptor derived from transgenic mice harboring SV40 large T-antigen gene

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)47244-2 ◽

1995 ◽

Vol 67 ◽

pp. 320

Author(s):

Akiko Mizuno ◽

Michio Takeda ◽

Kazuhito Fukuoka ◽

Hitoshi Endou

Keyword(s):

Transgenic Mice ◽

Proximal Tubule ◽

Cell Line ◽

T Antigen ◽

Large T Antigen ◽

Sv40 Large T Antigen ◽

V1a Receptor ◽

Antigen Gene ◽

Early Proximal Tubule

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Hepatocyte cell lines established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene

Experimental Cell Research ◽

10.1016/0014-4827(91)90478-d ◽

1991 ◽

Vol 197 (1) ◽

pp. 50-56 ◽

Cited By ~ 78

Author(s):

Nobuaki Yanai ◽

Misao Suzuki ◽

Masuo Obinata

Keyword(s):

Transgenic Mice ◽

Cell Lines ◽

Simian Virus 40 ◽

Simian Virus ◽

T Antigen ◽

Temperature Sensitive ◽

Large T Antigen ◽

Antigen Gene

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Novel Mast Cell Lines with Enhanced Proliferative and Degranulative Abilities Established from Temperature-Sensitive SV40 Large T Antigen Transgenic Mice

The Journal of Biochemistry ◽

10.1093/jb/mvj140 ◽

2006 ◽

Vol 140 (2) ◽

pp. 211-220 ◽

Cited By ~ 6

Author(s):

Masahiko Kanehira ◽

Tomonori Kaifu ◽

Kozue Maya ◽

Mitsuji Kaji ◽

Akira Nakamura ◽

...

Keyword(s):

Mast Cell ◽

Transgenic Mice ◽

Cell Lines ◽

T Antigen ◽

Temperature Sensitive ◽

Large T Antigen ◽

Sv40 Large T Antigen

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Development of two types of hepatocellular carcinoma in transgenic mice carrying the SV40 large T-antigen gene

Carcinogenesis ◽

10.1093/carcin/12.11.2059 ◽

1991 ◽

Vol 12 (11) ◽

pp. 2059-2062 ◽

Cited By ~ 12

Author(s):

Kimi Araki ◽

Okio Hino ◽

Jun-ichi Miyazaki ◽

Ken-ichi Yamamura

Keyword(s):

Hepatocellular Carcinoma ◽

Transgenic Mice ◽

T Antigen ◽

Large T Antigen ◽

Sv40 Large T Antigen ◽

Antigen Gene

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Immortalization of subpopulations of respiratory epithelial cells from transgenic mice bearing SV40 large T antigen

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1994.267.3.l309 ◽

1994 ◽

Vol 267 (3) ◽

pp. L309-L317 ◽

Cited By ~ 6

Author(s):

K. Ikeda ◽

J. C. Clark ◽

C. J. Bachurski ◽

K. A. Wikenheiser ◽

J. Cuppoletti ◽

...

Keyword(s):

Transgenic Mice ◽

Cell Lines ◽

Chloride Transport ◽

Short Circuit ◽

Surfactant Protein ◽

T Antigen ◽

Large T Antigen ◽

Sv40 Large T Antigen ◽

Necrosis Factor Alpha ◽

Low Levels

Murine lung epithelial (MLE) cell lines were produced from lung tumors derived from transgenic mice bearing the viral oncogene, SV40 large T antigen, under transcriptional control of the promoter-enhancer region of the human surfactant protein C (SP-C) gene. Cells were selected on the basis of increased murine cystic fibrosis transmembrane conductance regulator (mCFTR) mRNA content and were dilution cloned to produce distinct immortalized epithelial cell lines. MLE-13a3 cell lines expressing high levels of mCFTR mRNA also expressed apolipoprotein J (apoJ) mRNA, a developmentally regulated glycoprotein expressed preferentially in fetal lung. SP-A, -B, and -C were not detected or were present at low levels in the MLE cells that contained abundant CFTR and apoJ mRNA. In contrast, MLE cells, cloned on the basis of abundant surfactant protein mRNAs, expressed apoJ and mCFTR mRNAs at low levels. Forskolin-stimulated short-circuit current, typical of CFTR-mediated chloride transport activity, was generated by monolayers of subclones of the MLE-13a3 cell lines. Tumor necrosis factor-alpha stimulated mCFTR mRNA, whereas dexamethasone, retinoic acid, and phorbol ester had no effect on the levels of mCFTR mRNA in MLE-13a3 cells.

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