Discovery of novel NGS-mined microsatellite markers and an exploratory analysis of genetic differentiation between two Western Atlantic populations ofCardisoma guanhumiLatreille, 1825 (Decapoda: Brachyura: Gecarcinidae)

2019 ◽  
Vol 39 (2) ◽  
pp. 181-185 ◽  
Author(s):  
Sulimary O Gomes ◽  
Isis G B Souza ◽  
Michelli F Santos ◽  
Geice R Silva ◽  
Marc Albrecht ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Exploratory Analysis ◽  
Western Atlantic

scholarly journals Population Genetics of the Red Rock Lobster,  Jasus edwardsii

2021 ◽  
Author(s):  
◽  
Luke Thomas
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
New Zealand ◽  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Rocky Reef ◽  
Rock Lobster ◽  
Wide Range ◽  
Jasus Edwardsii ◽  
Polymorphic Microsatellite

<p>Understanding patterns of gene flow across a species range is a vital component of an effective fisheries management strategy. The advent of highly polymorphic microsatellite markers has facilitated the detection of fine-scale patterns of genetic differentiation at levels below the resolving power of earlier techniques. This has triggered the wide-spread re-examination of population structure for a number of commercially targeted species. The aims of thesis were to re-investigate patterns of gene flow of the red rock lobster Jasus edwardsii throughout New Zealand and across the Tasman Sea using novel microsatellite markers. Jasus edwardsii is a keystone species of subtidal rocky reef system and supports lucrative export markets in both Australia and New Zealand. Eight highly polymorphic microsatellite markers were developed from 454 sequence data and screened across a Wellington south coast population to obtain basic diversity indices. All loci were polymorphic with the number of alleles per locus ranging from 6-39. Observed and expected heterozygosity ranged from 0.563-0.937 and 0.583-0.961, respectively. There were no significant deviations from Hardy-Weinberg equilibrium following standard Bonferroni corrections. The loci were used in a population analysis of J. edwardsii that spanned 10 degrees of latitude and stretched 3,500 km across the South Pacific. The analysis rejected the null-hypothesis of panmixia based on earlier mDNA analysis and revealed significant population structure (FST=0.011, RST=0.028) at a wide range of scales. Stewart Island was determined to have the highest levels of genetic differentiation of all populations sampled suggesting a high degree of reproductive isolation and self-recruitment. This study also identified high levels of asymmetric gene flow from Australia to New Zealand indicating a historical source-sink relationship between the two countries. Results from the genetic analysis were consistent with results from oceanographic dispersal models and it is likely that the genetic results reflect historical and contemporary patterns of Jasus edwardsii dispersal and recruitment throughout its range.</p>

Limited genetic differentiation in Labeo rohita (Hamilton 1822) populations as revealed by microsatellite markers

2014 ◽  
Vol 57 ◽  
pp. 427-431 ◽  
Author(s):  
L. Sahoo ◽  
B.P. Sahu ◽  
Sofia P. Das ◽  
Subrat K. Swain ◽  
D. Bej ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Labeo Rohita

scholarly journals Genetic Differentiation Between Segugio Dell’Appeinnino and Segugio Maremmano Dog Breeds Assessed by Microsatellite Markers

2015 ◽  
Vol 14 (3) ◽  
pp. 3809 ◽  
Author(s):  
Vincenzo La Manna ◽  
Emiliano Lasagna ◽  
Simone Ceccobelli ◽  
Piera Di Lorenzo ◽  
Attilio M. De Cosmo ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Microsatellite Markers

From the laboratory to the wild: salinity-based genetic differentiation of the European sea bass (Dicentrarchus labrax) using gene-associated and gene-independent microsatellite markers

2015 ◽  
Vol 162 (3) ◽  
pp. 515-538 ◽  
Author(s):  
Bruno Guinand ◽  
Nolwenn Quéré ◽  
Erick Desmarais ◽  
Jacques Lagnel ◽  
Costas S. Tsigenopoulos ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
European Sea Bass

scholarly journals Development and Characterization of Novel Microsatellite Markers for a Dominant Parasitoid (Stethynium Empoasca) in Tea Plantations Using High-throughput Sequencing

2020 ◽  
Author(s):  
Jinyu Li ◽  
Longqing Shi ◽  
Jie Chen ◽  
Minsheng You ◽  
Shijun You
Keyword(s):  
Biological Control ◽  
Gene Flow ◽  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Control Strategies ◽  
Null Alleles ◽  
Individual Gene ◽  
Tea Plantations

Abstract Background: Stethynium empoasca is an egg parasitoid of the predominant tea pest, and is regarded as the most promising candidate for both augmentative and conservative biological control in tea plantations. However, little is presently known about its biology, ecology, and genetics. Methods: Novel microsatellite markers were developed for S. empoasca from transcriptome sequences generated using high-throughput sequencing. The identified markers were then validated and characterized using 98 individuals from five geographically distinct populations through the tests of Hardy–Weinberg equilibrium and linkage disequilibrium as well as the analyses of genetic diversity, genetic differentiation, and gene flow.Results: A total of 54,520 microsatellites were identified from 117Mb clean sequences. By assessing with five geographical populations, a total of 18 loci were demonstrated to be polymorphic, stable, and repetitive. The genetic variation over the 18 loci varied with allele number ranging from 2 to 7, polymorphism information content from 0.234 to 0.634; and observed and expected heterozygosity from 0.166 to 0.686 and 0.276 to 0.753, respectively. Meanwhile, the 18 loci showed a low frequency of null alleles (0 – 0.107), and the inbreeding coefficient revealed a range from −0.143 to 0.173, with the exception of loci Ste36 (0.4076). Based on analysis of these 18 loci, the assessed populations showed low to moderate levels of genetic differentiation, in which individuals clearly grouped into two clusters. And the recent dispersal rates between the geographically distinct populations were identified to be much lower (0.011 – 0.250) than the individual gene exchange rates within a population (0.683 – 0.939).Conclusion: The identified 18 microsatellite markers could reveal a pattern of spatial structure and gene flow in S. empoasca populations according to geographic variability. This work provides an important basis for future studies on how these markers can be used in studies of the biology, genetics, and ecology of this important parasitoid. The findings can further provide important information for the development of biological control strategies in tea plantations. Additionally, our study reaffirms the importance and efficiency of high-throughput sequencing in microsatellite marker development for non-model species lacking reference genome information.

scholarly journals Development and application of 14 microsatellite markers in the burying beetle Nicrophorus vespilloides reveals population genetic differentiation at local spatial scales

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3278 ◽  
Author(s):  
Sonia Pascoal ◽  
Rebecca M. Kilner
Keyword(s):  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Parental Care ◽  
Social Evolution ◽  
Spatial Scales ◽  
Burying Beetle ◽  
Genetic Structuring ◽  
Population Genetic Differentiation ◽  
Nicrophorus Vespilloides ◽  
Limited Dispersal

Burying beetles (genus Nicrophorus) are relatively rare among insects in providing sophisticated parental care. Consequently, they have become model species in research analysing social evolution, the evolution of parental care and mating systems. We used the recently published N. vespilloides genome and transcriptome to develop microsatellite markers. Specifically, we developed 14 polymorphic markers with five to 13 alleles per locus and used them to investigate levels of genetic differentiation in four south Cambridgeshire (UK) populations of N. vespilloides, separated by 21 km at most. The markers revealed significant genetic structuring among populations (global FST = 0.023) with all but one of the pairwise comparisons among populations being significant. The single exception was the comparison between the two closest populations, which are approximately 2.5 km apart. In general, the microsatellite markers showed lower observed heterozygosity than expected. We infer that there is limited dispersal between populations and potentially also some inbreeding within them and suggest that this may be due to habitat fragmentation. We discuss these results in the context of recent laboratory experiments on inbreeding and beetle flight.

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