Bloodmeal Identification in Field-Collected Sand Flies From Casa Branca, Brazil, Using the Cytochrome b PCR Method

2017 ◽  
Vol 54 (4) ◽  
pp. 1049-1054 ◽  
Author(s):  
G.M.L. Carvalho ◽  
F. D. Rêgo ◽  
A. Tanure ◽  
A.C.P. Silva ◽  
T. A. Dias ◽  
...  
Keyword(s):  
Cytochrome B ◽  
Sand Flies ◽  
Pcr Method ◽  
Bloodmeal Identification

scholarly journals First Report of Phlebotomine Sand Flies (Diptera: Psychodidae) in Kansas and Missouri, and a PCR Method to Distinguish Lutzomyia shannoni From Lutzomyia vexator

2012 ◽  
Vol 49 (6) ◽  
pp. 1460-1465 ◽  
Author(s):  
Ju-Lin Weng ◽  
Samantha L. Young ◽  
David M. Gordon ◽  
David Claborn ◽  
Christine Petersen ◽  
...  
Keyword(s):  
Sand Flies ◽  
Phlebotomine Sand Flies ◽  
First Report ◽  
Pcr Method

scholarly journals Diversity patterns, Leishmania DNA detection, and bloodmeal identification of Phlebotominae sand flies in villages in northern Colombia

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190686 ◽  
Author(s):  
Camila González ◽  
Cielo León ◽  
Andrea Paz ◽  
Marla López ◽  
Gisell Molina ◽  
...  
Keyword(s):  
Dna Detection ◽  
Sand Flies ◽  
Diversity Patterns ◽  
Bloodmeal Identification

scholarly journals Identification of blood meals in field captured sand flies by a PCR-RFLP approach based on cytochrome b gene

Acta Tropica ◽  
2015 ◽  
Vol 152 ◽  
pp. 96-102 ◽  
Author(s):  
Estela González ◽  
Montserrat Gállego ◽  
Ricardo Molina ◽  
Alba Abras ◽  
M. Magdalena Alcover ◽  
...  
Keyword(s):  
Cytochrome B ◽  
Cytochrome B Gene ◽  
Sand Flies ◽  
Pcr Rflp ◽  
Blood Meals

scholarly journals Amino acid diversity on the basis of cytochrome b gene in Kacang and Ettawa Grade goats

2017 ◽  
Vol 42 (3) ◽  
pp. 135 ◽  
Author(s):  
D. A. Lestari ◽  
S. Sutopo ◽  
E. Kurnianto
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Genetic Marker ◽  
Cytochrome B ◽  
Genomic Dna ◽  
Cyt B ◽  
Specific Amino Acid ◽  
Pcr Method ◽  
Cyt B Gene ◽  
Amino Acid Diversity

The objectives of study were to identify and assess the amino acid diversity of Cytochrome b (Cyt b) gene, genetic marker and characteristic of specific amino acid in Kacang and Ettawa Grade goat. Nineteen heads of Kacang goat (KG) and twelve heads of Ettawa Grade goat (EG) were purposively sampled. The genomic DNA was isolated by Genomic DNA Mini Kit (Geneaid) and amplified Cyt b using PCR method with CytbCapF and CytbCapR primers and was sequenced. The results showed that there were two specific amino acids that distinguish KG and EG goat with C. hircus and C. aegagrus and four specific amino acids that distinguish KG and EG goat with C. falconeri, but there were no specific amino acids can be used as a genetic marker to distinguish between Kacang and EG goat. In conclusion, specific amino acids in Cyt b gene can be used as a genetic marker among KG and EG goat with 3 goat others comparator.

scholarly journals Population genetics analysis of Phlebotomus papatasi sand flies from Egypt and Jordan based on mitochondrial cytochrome b haplotypes

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Catherine M. Flanley ◽  
Marcelo Ramalho-Ortigao ◽  
Iliano V. Coutinho-Abreu ◽  
Rami Mukbel ◽  
Hanafi A. Hanafi ◽  
...  
Keyword(s):  
Population Genetics ◽  
Cytochrome B ◽  
Sand Flies ◽  
Mitochondrial Cytochrome ◽  
Phlebotomus Papatasi ◽  
Mitochondrial Cytochrome B

scholarly journals Marcadores moleculares como herramientas en la identificación y análisis genético de especies vectores de interés en salud pública, género Lutzomyia (Grupo Verrucarum Theodor, 1965)

2016 ◽  
Vol 18 (1) ◽  
pp. 138
Author(s):  
Carol Yovanna Rosero-Galindo ◽  
Franco Andrés Montenegro Coral ◽  
Juan Pablo García López
Keyword(s):  
Public Health ◽  
Molecular Markers ◽  
Cytochrome B ◽  
Relevant Literature ◽  
Cytochrome B Gene ◽  
Sand Flies ◽  
Public Health Importance ◽  
Revisión Sistemática ◽  
Para Determinar ◽  
Pathogenic Agents

ResumenIntroducción: Los insectos flebotomíneos del grupo Verrucarum, son vectores de interés en salud pública dada su participación en la transmisión de las enfermedades tropicales: leishmaniasis y bartonelosis. El empleo de marcadores moleculares como herramienta de identificación y análisis genético de especies que se comportan como vectores potenciales y confirmados, muestran un uso ampliamente difundido, que genera un conjunto de información que se actualiza constantemente, más aun cuando la identificación de estas especies, es prioritaria en los países donde estas enfermedades ocurren de forma recurrente. Objetivo: Se presenta una revisión sistemática con el objetivo de recopilar y presentar una actualización sobre el uso de marcadores moleculares empleados en especies del grupo Verrucarum. Materiales y métodos: Esta búsqueda se efectuó en un rango de literatura relevante publicada en un periodo de 24 años que incluyó 23 artículos en la temática de genética y biología molecular de flebótomos y 39 artículos de otras áreas como sistemática, ecología de flebótomos y microbiología de agentes patógenos asociados. Resultados: Los resultados muestran el empleo de los genes que codifica para el ARN ribosomal 18S y para el citocromo oxidasa subunidad I como los más efectivos para observar las relaciones filogenéticas entre especies del grupo Verrucarum; el uso del gen citocromo b como carácter taxonómico alternativo para identificar correctamente los flebótomos y, el uso de secuencias espaciadoras de la transcripción interna del ARN ribosomal y el gen citocromo b para determinar la variación genética intra e interespecífica de las poblaciones. Conclusión: Esta revisión contribuirá a estudios filogenéticos de vectores de importancia en salud pública. AbstractIntroduction: The phlebotomine flies of the Verrucarum group are vectors of interest in public health because of their participation in the transmission of the tropical diseases: leishmaniasis and bartonellosis. The use of molecular markers, as a tool for the identification and genetic analysis of species that behave as potential and confirmed vectors, show a widely disseminated use that generates a set of information that is constantly updated, even more when the identification of these species is a priority in the countries where these diseases occur on a recurring basis. Objective: The paper presents a systematic review that aims at compiling and presenting an update on the use of molecular markers employed in species of the Verrucarum group. Materials and methods: This search was conducted in a range of relevant literature published in a period of 24 years which included 23 articles on the subject of genetics and molecular biology of sand flies, and 39 articles from other areas such as systematic, ecology of sand flies and microbiology of related pathogenic agents. Results: The results show the use of genes coding for ribosomal 18S RNA and cytochrome oxidase subunit I as the most effective to observe the phylogenetic relationships among species of the Verrucarum group; the use of the cytochrome b gene as alternative taxonomic character to correctly identify the sand flies, and the use of spacer sequences of the internal transcript of the ribosomal RNA and the Cytochrome b gene to determine the genetic variation of intra- and inter-specific populations. Conclusions: This review will contribute to phylogenetic studies of vectors of public health importance.

Some Observations on Intra-Mitochondrial Crystals

1977 ◽  
Vol 35 ◽  
pp. 406-407
Author(s):  
J. A. Clarke ◽  
D. N. Landon ◽  
P. R. Ward
Keyword(s):  
Cytochrome B ◽  
Mitochondrial Myopathy ◽  
Crystallographic Analysis ◽  
Mitochondrial Membranes ◽  
Electron Microscopes ◽  
Muscle Biopsies

Intra-mitochondrial crystals have been noted in muscle biopsies from patients in a wide variety of diseased states. As far as we are aware, none of these crystals have been subjected to detailed crystallographic analysis. Recently, similar crystals were observed in a biopsy from a patient with a mitochondrial myopathy, characterised by a deficiency in reducible cytochrome b (Morgan-Hughes, J. A., Darveniza, P., Kahn, S. N., Landon, D. N., Sherratt, R. M., Land, J. M. and Clark, J. B., 1977, Brain, In Press). Aldehyde-fixed, osmicated resin imbedded material was examined using Siemens, JEOL and Phillips electron microscopes with goniometer specimen stages. The crystals generally lay between the outer and inner mitochondrial membranes and measured 1 - 3 μm in length and 0.1 - 0.3 μm in width. Characteristically, these crystals revealed specific periodicities.

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

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