Detection of Multiple Blood Feeding in Aedes aegypti (Diptera: Culicidae) During a Single Gonotrophic Cycle Using a Histologic Technique

1993 ◽  
Vol 30 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Thomas W. Scott ◽  
Gary G. Clark ◽  
Leslie H. Lorenz ◽  
Priyanie H. Amerasinghe ◽  
Paul Reiter ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Blood Feeding ◽  
Gonotrophic Cycle ◽  
Multiple Blood ◽  
Histologic Technique

scholarly journals Characterization of a vasodilator from the salivary glands of the yellow fever mosquito Aedes aegypti

1992 ◽  
Vol 165 (1) ◽  
pp. 61-71 ◽  
Author(s):  
J. M. Ribeiro
Keyword(s):  
Salivary Gland ◽  
Aedes Aegypti ◽  
Salivary Glands ◽  
Posterior Part ◽  
Aortic Ring ◽  
Blood Feeding ◽  
Reversed Phase ◽  
Relative Molecular Mass ◽  
Vasodilatory Activity ◽  
Carboxy Terminal

Salivary gland homogenates and oil-induced saliva of the mosquito Aedes aegypti dilate the rabbit aortic ring and contract the guinea pig ileum. The vasodilatory activity is endothelium-dependent, heat-stable, sensitive to both trypsin and chymotrypsin treatments, and both smooth muscle activities cross-desensitize to the tachykinin peptide substance P. Both bioactivities co-elute when salivary gland homogenates are fractionated by reversed-phase HPLC. Molecular sieving chromatography indicates a relative molecular mass of 1400. A monoclonal antibody specific to the carboxy terminal region of tachykinins reacts with material in the posterior part of the central lobe of paraformaldehyde-fixed salivary glands. The presence of a vasodilatory peptide of the tachykinin family in the salivary glands of A. aegypti is proposed and its role in blood feeding is discussed.

Confocal Analysis of the Distribution and Persistence of Sindbis Virus (TaV-GFP) Infection in Midguts of Aedes aegypti Mosquitoes

2020 ◽  
Vol 26 (2) ◽  
pp. 267-274
Author(s):  
Jason J. Saredy ◽  
Florence Y. Chim ◽  
Zoë L. Lyski ◽  
Yani P. Ahearn ◽  
Doria F. Bowers
Keyword(s):  
Aedes Aegypti ◽  
Sindbis Virus ◽  
Fluorescent Protein ◽  
Blood Feeding ◽  
Target Tissue ◽  
Secondary Target ◽  
Posterior Midgut ◽  
Infected Cells ◽  
Green Fluorescent ◽  
Global Threat

AbstractBiological transmission of arthropod-borne viruses (arboviruses) to vertebrate hosts by hematophagous insects poses a global threat because such arboviruses can result in a range of serious public health infectious diseases. Sindbis virus (SINV), the prototype Alphavirus, was used to track infections in the posterior midgut (PMG) of Aedes aegypti adult mosquitoes. Females were fed viremic blood containing a virus reporter, SINV [Thosea asigna virus-green fluorescent protein (TaV-GFP)], that leaves a fluorescent signal in infected cells. We assessed whole-mount PMGs to identify primary foci, secondary target tissues, distribution, and virus persistence. Following a viremic blood meal, PMGs were dissected and analyzed at various days of post blood-feeding. We report that virus foci indicated by GFP in midgut epithelial cells resulted in a 9.8% PMG infection and a 10.8% dissemination from these infected guts. The number of virus foci ranged from 1 to 3 per individual PMG and was more prevalent in the PMG-middle > PMG-frontal > PMG-caudal regions. SINV TaV-GFP was first observed in the PMG (primary target tissue) at 3 days post blood-feeding, was sequestered in circumscribed foci, replicated in PMG peristaltic muscles (secondary target tissue) following dissemination, and GFP was observed to persist in PMGs for 30 days postinfection.

Blood Feeding Status, Gonotrophic Cycle and Survivorship of Aedes (Stegomyia) aegypti (L.) (Diptera: Culicidae) Caught in Churches from Merida, Yucatan, Mexico

2017 ◽  
Vol 46 (6) ◽  
pp. 622-630 ◽  
Author(s):  
C M Baak-Baak ◽  
A Ulloa-Garcia ◽  
N Cigarroa-Toledo ◽  
J C Tzuc Dzul ◽  
C Machain-Williams ◽  
...  
Keyword(s):  
Blood Feeding ◽  
Gonotrophic Cycle

Sex before or after blood feeding: Mating activities of Aedes aegypti males under conditions of different densities and female blood feeding opportunities

2019 ◽  
Vol 22 (1) ◽  
pp. 274-280 ◽  
Author(s):  
Hamady Dieng ◽  
Tomomitsu Satho ◽  
Fatimah Abang ◽  
Erida Wydiamala ◽  
Nur Faeza Abu Kassim ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Blood Feeding
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