Diet (n-3) Polyunsaturated Fatty Acid Content and Parity Affect Liver and Erythrocyte Phospholipid Fatty Acid Composition in Female Rats

Salinity is a known factor in shaping population dynamics and community structure through direct and indirect effects on aquatic ecosystems. Salinity changes further influence food webs through competition and predation. The responses of Moina macrocopa (Cladocera) collected from Setiu Wetland lagoon (Terengganu) was evaluated through manipulative laboratory experiments to understand the ability of M. macrocopa to tolerate high salinity stress. Specifically, the fatty acid composition, growth, survival, and reproduction of this cladocerans species was examined. Sodium chloride (NaCl) as used in the treatments water with the concentration 0, 4, 6, 8, 12, and 15 salinity. Fatty acid levels were determined using Gas Chromatography and Mass Spectrophotometry (GC-MS). The results indicated that optimal conditions produced the highest fatty acid content, especially the polyunsaturated fatty acid content, such as EPA (eicosapentaenoic acid), ALA (alpha-linoleic acid), ARA (arachidonic acid), and DHA (docosahexaenoic acid). Furthermore, M. macrocopa survival was best at salinity 0, with a percentage of 98%, whereas the opposite occurred at salinity 15, with approximately 20% of viable animals surviving. Besides, M. macrocopa also showed the highest reproduction rate at salinity 0 (e.g., average initial age of reproduction, 4.33 ± 0.58 days) compared with other salinities level. Interestingly, the difference in growth at different salinities was not evident, an unusual finding when considering adverse effects such as osmoregulation pressure on the organism. Based on the results, we conclude that M. macrocopa can only tolerate salinity below salinity 8 and cannot withstand stressful environmental conditions associated with salinities above 8.

Download Full-text

Fatty Acid Composition of Three Marine Fishes of the Bay of Bengal

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v41i1.268 ◽

1970 ◽

Vol 41 (1) ◽

pp. 47-54 ◽

Cited By ~ 1

Author(s):

Habibur R Bhuiyan ◽

KK Nath ◽

P Seal ◽

Mir Esharul Hossain

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Essential Fatty Acid ◽

Acid Composition ◽

Polyunsaturated Fatty Acid ◽

Bay Of Bengal ◽

Fatty Acid Content ◽

Marine Fishes ◽

Gas Liquid Chromatography ◽

Polyunsaturated Fatty Acid Content

Oil samples collected from three fishes namely Sting Ray (Dasyatis uarrnak), Anchovy (Setipinna taty), and Eel (Congresox talabonoides) from the Bay of Bengal were analyzed to determine their fatty acid composition with the aid of Gas Liquid Chromatography (GLC). The saturated fatty acid contents were 52.95, 45.28 and 52.29 %; and the unsaturated fatty acid contents were 43.97, 54.72 and 33.39 % in D. uarnak, S. taty and C. talabonoides respectively. The highest polyunsaturated fatty acid content was found in S. taty (13.65 %) and the lowest was in D. uarnak (8.06 %). The ψ-3 fatty acid contents were 4.58, 3.65 and 9.91 % and the ψ-6 fatty acid contents were 3.48, 10.00 and 5.50 % in D. uarnak. S. taty and C. talabonoides respectively. Two essential fatty acid linoleic acid (4.12 %) and arachidonic acid (5.32 %) contents were found higher in S. taty whereas another essential fatty acid linolenic acid (8.98 %) found higher in C. talabonoides. Another important polyunsaturated fatty acid eicosapentaenoic acid (2.12%) found higher in D. uarnak. Bangladesh J. Sci. Ind. Res. 41(1-2), 47-54, 2006

Download Full-text

Dietary Lipids and Phospholipid Fatty Acid Composition in Hepatic Micro-somes of Male and Female Rats

Agricultural and Biological Chemistry ◽

10.1080/00021369.1989.10869682 ◽

1989 ◽

Vol 53 (9) ◽

pp. 2501-2503

Author(s):

Galit Levin ◽

Shoshana Mokady ◽

Uri Cogan

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Phospholipid Fatty Acid ◽

Dietary Lipids ◽

Female Rats ◽

Phospholipid Fatty Acid Composition ◽

Male And Female ◽

Male And Female Rats

Download Full-text

Effect of reduced maternal protein intake in pregnancy in the rat on the fatty acid composition of brain, liver, plasma, heart and lung phospholipids of the offspring after weaning

British Journal Of Nutrition ◽

10.1079/bjn2003909 ◽

2003 ◽

Vol 90 (2) ◽

pp. 345-352 ◽

Cited By ~ 29

Author(s):

Graham C. Burdge ◽

Emmanuelle Delange ◽

Ludivine Dubois ◽

Rebecca L. Dunn ◽

Mark A. Hanson ◽

...

Keyword(s):

Arachidonic Acid ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Docosahexaenoic Acid ◽

Acid Composition ◽

Acid Content ◽

Protein Intake ◽

Female Rats ◽

Arachidonic Acid Content ◽

Phospholipid Fatty Acid Composition

Reduced protein intake during pregnancy decreased maternal hepatic and plasma docosahexaenoic acid concentrations and impaired docosahexaenoic acid accumulation into fetal brain in the rat. The present study investigated whether restriction of maternal protein intake during pregnancy in the rat alters membrane phospholipid fatty acid composition in the offspring after weaning. Female rats (six per group) were mated and fed diets containing either 180 or 90 g protein/kg throughout pregnancy. Mothers were transferred to standard chow after delivery and the litters reduced to eight pups. Weaning was at 28 d and pups were killed 5 to 6 d later. Tissue weights or membrane total phosphatidylcholine (PC) and phosphatidylethanolamine (PE) concentrations in the offspring did not differ between dietary groups. There were significant differences between the 180 and 90 g/kg groups in liver, brain, lung and heart fatty acid composition that differed between tissues and phospholipid classes. For example, docosahexaenoic and arachidonic acid concentrations were 23 and 10 % lower respectively in hepatic PC, but not PE, in the 90 g/kg group. In brain, docosahexaenoic acid concentration was 17 % lower in PC, but not PE, while arachidonic acid content was 21 % greater in PE but unchanged in PC. The greatest differences were in unsaturated fatty acids, which suggests alterations to desaturase activities and/or the specificity of phospholipid biosynthesis. These results suggest that restricted maternal protein intake during pregnancy results in persistent alterations to membrane fatty acid content.

Download Full-text