scholarly journals Molecular Detection of Epstein–Barr Virus, Human Herpes Virus 6, Cytomegalovirus, and Hepatitis B Virus in Patients with Multiple Sclerosis

2020 ◽  
Vol 12 (3) ◽  
pp. 171-177
Author(s):  
Mohsen Asouri ◽  
Mohammad Ali Sahraian ◽  
Morteza Karimpoor ◽  
Sadegh Fattahi ◽  
Nima Motamed ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Control Group ◽  
Case Group ◽  
Control Groups ◽  
Barr Virus ◽  
Human Herpes Virus ◽  
B Virus ◽  
Human Herpes Virus 6 ◽  
Epstein Barr ◽  
And Control

BACKGROUND Multiple sclerosis (MS) is a chronic disease with significant morbidity. A wide spectrum of risk factors has been suggested that triggers the development of MS. Among them, several viral infections have been implicated to play a role in MS pathogenesis. We aimed to evaluate the relationship between viral diseases, including Epstein–Barr virus (EBV), human herpes virus 6 (HHV-6), cytomegalovirus (CMV), and hepatitis B virus (HBV) and MS in the present case-control study. METHODS About 100 patients with confirmed MS and age- and sex-matched individuals were selected as case and control groups, respectively. The patients were randomly selected from individuals diagnosed by neurologists based on the clinical signs and symptoms and imaging procedures. RESULTS More than 100 patients with MS and patients who were referred for other causes were analyzed for the presence of DNA of EBV, HHV6, CMV, and HBV separately. 9.37% of the control group had a positive test for the DNA of EBV in a real-time polymerase chain reaction (PCR), while the frequency of positive test result was zero in the case group (p = 0.0012). HBV DNA was not detected in both the case and control groups. The prevalence of CMV was 0.88 and zero in the control and case groups, respectively (p = 0.3410). For HHV6, 9.73 % of the control group had a positive result, while this test was positive in 5.88% of the patients with MS (p = 0.2959). CONCLUSION We detected a significantly higher number of individuals with DNA of EBV in their blood among the control group compared with the case group. In conclusion, the results suggest a surprisingly adverse association between MS and EBV, and no association was found between the presence of DNA of HBV, CMV, and HHV6 and MS.

scholarly journals Association of Epstein–Barr Virus and Cytomegalovirus Infections with Esophageal Carcinoma

2021 ◽  
Vol 14 (10) ◽  
Author(s):  
Mahin Ahangar Oskouee ◽  
Jamal Sarvari ◽  
Afagh Moattari ◽  
Ahmad Habibi ◽  
Amir Taher Eftehkar Sadat
Keyword(s):  
Esophageal Carcinoma ◽  
Epstein Barr Virus ◽  
Viral Infections ◽  
Control Group ◽  
Control Groups ◽  
Barr Virus ◽  
Potential Association ◽  
Epstein Barr ◽  
And Control ◽  
Cytomegalovirus Infections

Background: Given the fact that viral infections play an important role, either directly or indirectly, in around 20 percent of human cancers, this study aimed at investigating the potential association of Epstein–Barr virus (EBV) and cytomegalovirus (CMV) infections in esophageal cancer that is the sixth most common cause of cancer-related deaths. Methods: In this case-control study, a total of 200 paraffin-embedded biopsies of cancerous and benign esophageal tissues were gathered from the biopsy bank of Imam Reza Hospital, Tabriz, Iran in 2017. All samples were first deparaffinized, and then subjected to commercial DNA extraction. The quality of extracted DNA was evaluated by amplification of the beta globulin gene. Identification of EBV and CMV DNA was performed using primers designed for the EBER region of EBV and the immediate early (IE) region of the CMV genome, respectively. Results: The mean age of the subjects in the test and control groups was 52.2 (17.1) and 59.9 (18.9), respectively. The distribution of gender (male/female) in patient and control groups was 54/46 and 53/47, respectively. Our results showed that the frequency of EBV (P < 0.001) and CMV (P < 0.001) in cancerous samples was statistically higher than control group. Moreover, in the cancerous group the rate of EBV was significantly higher in the esophageal adenocarcinomas (EAC) sample (12 out of 70) than esophageal squamous cell carcinomas (ESCC) (0 out of 30) (P = 0.016) but, in the ESCC group, 17 out of 30 subjects were positive for CMV which was significantly higher in comparison with EAC patients (1 out of 70) (P < 0.001). Conclusions: Findings indicated that EBV and CMV might be contributed to the pathogenesis of EAC and ESCC types of esophageal carcinoma, respectively, although further studies are warranted.

Antibodies to Epstein-Barr virus-specific DNase in patients with nasopharyngeal carcinoma and control groups

1987 ◽  
Vol 23 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Jen-Yang Chen ◽  
Chien-Jen Chen ◽  
Mei-Ying Liu ◽  
Show-Mei Cho ◽  
Mow-Ming Hsu ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Control Groups ◽  
Barr Virus ◽  
Epstein Barr ◽  
And Control

Characterization of a human herpes virus-6 (HHV-6) and epstein-barr virus (EBV) associated leukemic cell line, J6-1

1994 ◽  
Vol 6 (3) ◽  
pp. 157-168 ◽  
Author(s):  
Wu Kefu ◽  
Janos Luka ◽  
Shantaram S. Joshi ◽  
Samuel J. Pirruccello ◽  
A. Masih ◽  
...  
Keyword(s):  
Cell Line ◽  
Epstein Barr Virus ◽  
Leukemic Cell ◽  
Human Herpes ◽  
Leukemic Cell Line ◽  
Barr Virus ◽  
Human Herpes Virus ◽  
Human Herpes Virus 6 ◽  
Epstein Barr

scholarly journals Macular Amyloidosis and Epstein-Barr Virus

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Yalda Nahidi ◽  
Naser Tayyebi Meibodi ◽  
Zahra Meshkat ◽  
Narges Nazeri
Keyword(s):  
Epstein Barr Virus ◽  
Globin Gene ◽  
Control Group ◽  
Barr Virus ◽  
Significant Difference ◽  
Macular Amyloidosis ◽  
Ebv Dna ◽  
Epstein Barr ◽  
And Control ◽  
Skin Samples

Background. Amyloidosis is extracellular precipitation of eosinophilic hyaline material of self-origin with special staining features and fibrillar ultrastructure. Macular amyloidosis is limited to the skin, and several factors have been proposed for its pathogenesis. Detection of Epstein-Barr virus (EBV) DNA in this lesion suggests that this virus can play a role in pathogenesis of this disease.Objective. EBV DNA detection was done on 30 skin samples with a diagnosis of macular amyloidosis and 31 healthy skin samples in the margin of removed melanocytic nevi by using PCR.Results. In patients positive for beta-globin gene in PCR, BLLF1 gene of EBV virus was positive in 23 patients (8 patients in case and 15 patients in the control group). There was no significant difference in presence of EBV DNA between macular amyloidosis (3.8%) and control (23.8%) groups (P=0.08).Conclusion. The findings of this study showed that EBV is not involved in pathogenesis of macular amyloidosis.

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