Effects of Inhibitors of Protein and Nucleic Acid Synthesis on the Expression of H-2 and Moloney Leukemia Virus-Determined Cell-Surface Antigens on Cultured Murine Lymphoma Cells2

1972 ◽  
Keyword(s):  
Nucleic Acid ◽  
Cell Surface ◽  
Leukemia Virus ◽  
Surface Antigens ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Cell Surface Antigens ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Moloney Leukemia Virus

scholarly journals Antigenic modulation of Friend virus erythroleukemic cells in vitro by serum from mice with dormant erythroleukemia.

1977 ◽  
Vol 146 (2) ◽  
pp. 520-534 ◽  
Author(s):  
E V Genovesi ◽  
P A Marx ◽  
E F Wheelock
Keyword(s):  
Cell Surface ◽  
Leukemia Virus ◽  
Surface Antigens ◽  
Immune Serum ◽  
Mouse Serum ◽  
Cell Surface Antigens ◽  
Antigenic Modulation ◽  
In Cells ◽  
Erythroleukemic Cells

Friend leukemia virus (FLV) erythroleukemic cells cultured in medium containing FLV-immune serum from dormant FLV-infected mice undergo modulation of FLV cell surface antigens. Modulation was determined by an increased resistance to FLV antibody-mediated complement-dependent lysis and was associated temporally with the capping of FLV-immune complexes at the cell surface. Modulated cells regained their susceptibility to FLV antibody-mediated complement-dependent lysis when transferred to medium containing normal mouse serum. After 48 h of culture in FLV-immune serum, 26% of the FLV erythroleukemic cells were devoid of FLV cell surface antigens as demonstrated by immunofluoresence. Antigenic modulation occurred to a greater extent in cells maintained in logarithmic growth than in cells in GO or resting phase. FLV-antigenic modulation is discussed as a possible mechanism by which antibody induces and maintains FLV-transformed cells in a dormant state.

scholarly journals Cell surface antigens of chemically induced sarcomas of the mouse. I. Murine leukemia virus-related antigens and alloantigens on cultured fibroblasts and sarcoma cells: description of a unique antigen on BALB/c Meth A sarcoma.

1977 ◽  
Vol 146 (3) ◽  
pp. 720-734 ◽  
Author(s):  
A B DeLeo ◽  
H Shiku ◽  
T Takahashi ◽  
M John ◽  
L J Old
Keyword(s):  
Cell Surface ◽  
Cell Lines ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
Cultured Fibroblasts ◽  
Chemically Induced ◽  
Sarcoma Cells ◽  
Murine Leukemia

As background for a serological definition of the unique antigens of chemically induced sarcomas, we have typed a series of fibroblast and sarcoma cell lines of BALB/c and C57BL/6 origin by cytoxicity and absorption tests for murine leukemia virus (MuLV)-related cell surface antigens and known alloantigens. 7 of the 17 cultured lines expressed the range of cell surface antigens associated with MuLV (GIX, GCSA, gp70, p30), and this was invariably associated with MuLV production. In nonproducer lines of C57BL/6 (but not BALB/c) origin, a MuLV-gp70-like molecule was found on the surface of fibroblasts and sarcoma cells. The alloantigenic phenotype of these MuLV+ and MuLV- cell lines was H-2D+, H-2K+, Thy-1.2+ or -, PC.1+ or -, Lyt-1.2-, Lyt-2.2-, Ia.7-, and TL.2-. A unique antigen was defined on the BALB/c ascites sarcoma Meth A with antisera prepared in BALB/c or (BALB/c X C57BL/6)F1 mice. Tissue culture lines derived from this tumor were MuLV-, which facilitated serological study of the antigen. Absorption analysis indicated that the antigen was restricted to Meth A; it could not be detected in normal or fetal BALB/c tissue MuLV+ or MuLV- fibroblast lines, 12 syngeneic or allogeneic sarcomas, or normal lymphoid cells from 13 different inbred mouse strains.

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