Determination of Benzo[a]pyrene Diol Epoxide--DNA Adducts in White Blood Cell DNA From Coke-Oven Workers: The Impact of Smoking

The analysis of cellular and humoral immunity indicators in the blood of patients with purulent peritonitis (PP) with the determination of their diagnostic value while determining the stage of peritonitis was performed. The levels of CD3, CD4, CD8, CD4 / CD8, CD19, CD16, CD95, CD25, IgA, IgM, IgG during hospitalization of 126 patients with PP was investigated. Monitoring of immune responsiveness stated regular changes in cellular and humoral immunity caused by the influence of cytokine profile, describing immunosuppression, the extent of which depends on the length and stage of PP. The progressive deepening of PP stages (in reactive and toxic stage) led to an increase in white blood cell count, CD95, due to the influence of dose-dependent increase in IL-6, CD19, IgM, G, and NK-cells (CD16) and CD25, the dynamics of which is closely related and dependent of IL-2.This was accompanied by a progressive lymphocytopenia, a decrease in CD3, CD4, which at constant or slightly increasing concentration led to decrease in CD8 IRI (CD4 / CD8) caused by the impact of immunosuppressive IL-6, stepwise reduction of IgA. Severe secondary immunodeficiency was observed in patients with end-stage PP and lymphocytopenia progressed along with the growth of white blood cell and marker CD95 with disruption of T-lymphocytes subsets (CD4 decrease at a constant or insignificant growth of CD8) with critical IRI indicators. The decrease in CD25, CD16 and CD19 dysfunction with sharp inhibition of the antibodies synthesis – decrease in IgM and G, A concentrations was also determined.

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Detection of benzo[a]pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in serum from coke oven workers.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.82.19.6672 ◽

1985 ◽

Vol 82 (19) ◽

pp. 6672-6676 ◽

Cited By ~ 105

Author(s):

C. C. Harris ◽

K. Vahakangas ◽

M. J. Newman ◽

G. E. Trivers ◽

A. Shamsuddin ◽

...

Keyword(s):

Dna Adducts ◽

Peripheral Blood ◽

Coke Oven ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes ◽

Diol Epoxide ◽

Coke Oven Workers

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Differences in DNA methylation of white blood cell types at birth and in adulthood reflect postnatal immune maturation and influence accuracy of cell type prediction

10.1101/399279 ◽

2018 ◽

Cited By ~ 3

Author(s):

Meaghan J Jones ◽

Louie Dinh ◽

Hamid Reza Razzaghian ◽

Olivia de Goede ◽

Julia L MacIsaac ◽

...

Keyword(s):

Dna Methylation ◽

Immune System ◽

Blood Cell ◽

Cord Blood ◽

White Blood Cell ◽

Blood Cells ◽

Cell Types ◽

Cell Type ◽

Cell Type Specific ◽

The Impact

AbstractBackgroundDNA methylation profiling of peripheral blood leukocytes has many research applications, and characterizing the changes in DNA methylation of specific white blood cell types between newborn and adult could add insight into the maturation of the immune system. As a consequence of developmental changes, DNA methylation profiles derived from adult white blood cells are poor references for prediction of cord blood cell types from DNA methylation data. We thus examined cell-type specific differences in DNA methylation in leukocyte subsets between cord and adult blood, and assessed the impact of these differences on prediction of cell types in cord blood.ResultsThough all cell types showed differences between cord and adult blood, some specific patterns stood out that reflected how the immune system changes after birth. In cord blood, lymphoid cells showed less variability than in adult, potentially demonstrating their naïve status. In fact, cord CD4 and CD8 T cells were so similar that genetic effects on DNA methylation were greater than cell type effects in our analysis, and CD8 T cell frequencies remained difficult to predict, even after optimizing the library used for cord blood composition estimation. Myeloid cells showed fewer changes between cord and adult and also less variability, with monocytes showing the fewest sites of DNA methylation change between cord and adult. Finally, including nucleated red blood cells in the reference library was necessary for accurate cell type predictions in cord blood.ConclusionChanges in DNA methylation with age were highly cell type specific, and those differences paralleled what is known about the maturation of the postnatal immune system.

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