scholarly journals Changes of the Immune Status in Patients with Purulent Peritonitis

2016 ◽  
Vol 23 (3) ◽  
Author(s):  
Serhiy I Savolyuk ◽  
Maksim A Gudz ◽  
Andriy A Zhmur
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Humoral Immunity ◽  
Diagnostic Value ◽  
Purulent Peritonitis ◽  
Stepwise Reduction ◽  
Cellular And Humoral Immunity ◽  
End Stage ◽  
The Impact

The analysis of cellular and humoral immunity indicators in the blood of patients with purulent peritonitis (PP) with the determination of their diagnostic value while determining the stage of peritonitis was performed. The levels of CD3, CD4, CD8, CD4 / CD8, CD19, CD16, CD95, CD25, IgA, IgM, IgG during hospitalization of 126 patients with PP was investigated. Monitoring of immune responsiveness stated regular changes in cellular and humoral immunity caused by the influence of cytokine profile, describing immunosuppression, the extent of which depends on the length and stage of PP. The progressive deepening of PP stages (in reactive and toxic stage) led to an increase in white blood cell count, CD95, due to the influence of dose-dependent increase in IL-6, CD19, IgM, G, and NK-cells (CD16) and CD25, the dynamics of which is closely related and dependent of IL-2.This was accompanied by a progressive lymphocytopenia, a decrease in CD3, CD4, which at constant or slightly increasing concentration led to decrease in CD8 IRI (CD4 / CD8) caused by the impact of immunosuppressive IL-6, stepwise reduction of IgA. Severe secondary immunodeficiency was observed in patients with end-stage PP and lymphocytopenia progressed along with the growth of white blood cell and marker CD95 with disruption of T-lymphocytes subsets (CD4 decrease at a constant or insignificant growth of CD8) with critical IRI indicators. The decrease in CD25, CD16 and CD19 dysfunction with sharp inhibition of the antibodies synthesis – decrease in IgM and G, A concentrations was also determined.

Determination of Benzo[a]pyrene Diol Epoxide--DNA Adducts in White Blood Cell DNA From Coke-Oven Workers: The Impact of Smoking

1990 ◽  
Vol 82 (11) ◽  
pp. 927-933 ◽  
Author(s):  
F. J. van Schooten ◽  
F. E. van Leeuwen ◽  
M. J. X. Hillebrand ◽  
M. E. de Rijke ◽  
A. A. M. Hart ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Dna Adducts ◽  
Coke Oven ◽  
Diol Epoxide ◽  
Coke Oven Workers ◽  
The Impact

Natural immunity factors in Polish mixed breed rabbits

2015 ◽  
Vol 18 (1) ◽  
pp. 19-28 ◽  
Author(s):  
B. Tokarz-Deptuła ◽  
P. Niedźwiedzka-Rystwej ◽  
M. Adamiak ◽  
B. Hukowska-Szematowicz ◽  
A. Trzeciak-Ryczek ◽  
...  
Keyword(s):  
Humoral Immunity ◽  
Peripheral Blood ◽  
Scientific Research ◽  
Natural Immunity ◽  
Immunological Status ◽  
Mixed Breed ◽  
Cellular And Humoral Immunity ◽  
The Impact

AbstractMixed-breed rabbits in Poland are widely used for diagnostic and scientific research and as utility animals, therefore there is a need to know their immunological status, as well as their haematological status. In this study natural immunity factors were analyzed in Polish mixed-breed rabbits and Polish mixed-breed rabbits with addition of blood of meet-breed, considering the impact of sex and season of the year (spring, summer, autumn, winter) using measurement of non-specific cellular and humoral immunity parameters in peripheral blood. The study has revealed that there is a variety between the two commonly used mixed-breed types of rabbits, especially when sex and season is concerned, which is crucial for using these animals in experiments.

scholarly journals Differences in DNA methylation of white blood cell types at birth and in adulthood reflect postnatal immune maturation and influence accuracy of cell type prediction

2018 ◽  
Author(s):  
Meaghan J Jones ◽  
Louie Dinh ◽  
Hamid Reza Razzaghian ◽  
Olivia de Goede ◽  
Julia L MacIsaac ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Immune System ◽  
Blood Cell ◽  
Cord Blood ◽  
White Blood Cell ◽  
Blood Cells ◽  
Cell Types ◽  
Cell Type ◽  
Cell Type Specific ◽  
The Impact

AbstractBackgroundDNA methylation profiling of peripheral blood leukocytes has many research applications, and characterizing the changes in DNA methylation of specific white blood cell types between newborn and adult could add insight into the maturation of the immune system. As a consequence of developmental changes, DNA methylation profiles derived from adult white blood cells are poor references for prediction of cord blood cell types from DNA methylation data. We thus examined cell-type specific differences in DNA methylation in leukocyte subsets between cord and adult blood, and assessed the impact of these differences on prediction of cell types in cord blood.ResultsThough all cell types showed differences between cord and adult blood, some specific patterns stood out that reflected how the immune system changes after birth. In cord blood, lymphoid cells showed less variability than in adult, potentially demonstrating their naïve status. In fact, cord CD4 and CD8 T cells were so similar that genetic effects on DNA methylation were greater than cell type effects in our analysis, and CD8 T cell frequencies remained difficult to predict, even after optimizing the library used for cord blood composition estimation. Myeloid cells showed fewer changes between cord and adult and also less variability, with monocytes showing the fewest sites of DNA methylation change between cord and adult. Finally, including nucleated red blood cells in the reference library was necessary for accurate cell type predictions in cord blood.ConclusionChanges in DNA methylation with age were highly cell type specific, and those differences paralleled what is known about the maturation of the postnatal immune system.

The Impact of High Anxiety Level on Cellular and Humoral Immunity in Mice

2010 ◽  
Vol 17 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Hassan Rammal ◽  
Jaouad Bouayed ◽  
Jaïro Falla ◽  
Naoual Boujedaini ◽  
Rachid Soulimani
Keyword(s):  
Humoral Immunity ◽  
Anxiety Level ◽  
High Anxiety ◽  
Cellular And Humoral Immunity ◽  
The Impact

Diagnostic value of blood cell sedimentation rate in gynecology. Rewnu (Zentr. f. Gyn., 1922, No. 49)

1923 ◽  
Vol 19 (4) ◽  
pp. 97-97
Author(s):  
V. Gruzdev
Keyword(s):  
Blood Cell ◽  
Sedimentation Rate ◽  
Malignant Tumors ◽  
Diagnostic Value

Determination of this rate makes it easy to distinguish malignant tumors (rate increased) from benign (rate normal), hemorrhagic tumors of uterine appendages (rate increased) from serous (rate normal), chronic inflammations of appendages (rate normal) from acute, especially naginous (sedimentation accelerated).

Prospective Study of Bone, lndium-111-Labeled White Blood Cell, and Gallium-67 Scanning for the Evaluation of Osteomyelitis in the Diabetic Foot

1996 ◽  
Vol 17 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Jeffrey E. Johnson ◽  
E. Jeff Kennedy ◽  
Michael J. Shereff ◽  
Neetin C. Patel ◽  
B. David Collier
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Diagnostic Value ◽  
Clinical Suspicion ◽  
Cell Uptake ◽  
Diabetic Patients ◽  
Bone Scanning ◽  
Diagnostic Efficacy ◽  
Radiographic Findings ◽  
Gallium 67

Twenty-two adult diabetic patients with clinical suspicion of foot and/or ankle infection were prospectively evaluated using radiography, technetium-99m methylene diphosphonate bone scanning (99Tc), indium-111-labeled leukocyte scanning (111In), and gallium-67 scanning (67Ga) to determine the presence of clinically suspected osteomyelitis. Biopsy for culture and histology was performed in 16 patients. The diagnosis of osteomyelitis was confirmed by biopsy in 12 patients. The remaining 10 patients had no evidence of osteomyelitis with long-term follow-up. 99mTc was snown to be of limited valued when used alone in these patients with peripheral neuropathy. 67Ga, either alone or in combination with 99mTc bone scanning, was of little diagnostic value and gave no additional information that was not available from 111In. The combination of three-phase 99mTc and 111In had the highest diagnostic efficacy (100% sensitivity, 80% specificity, and 91% accuracy), followed closely by 111In alone (100% sensitivity, 70% specificity, and 86% accuracy). We conclude that for adult diabetic patients with clinical suspicion of osteomyelitis but no radiographic findings of that disease, 111In alone is an appropriate nuclear medicine evaluation for ruling out infection if it is negative. However, if an area of 111In white blood cell uptake is present, a “simultaneous” 99mTc is often helpful in providing the anatomic correlation to differentiate osteomyelitis from infection that is limited to soft tissue.

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