scholarly journals Assessment of the effects of exposure to extremely low-frequency magnetic fields on MDCK epithelial cell lines under a controlled environment

2021 ◽  
Author(s):  
Gonzalo Domínguez ◽  
Eladio Cardiel ◽  
Elsa Sánchez ◽  
Pablo-Rogelio Hernández
Keyword(s):  
Magnetic Field ◽  
Cell Migration ◽  
Magnetic Fields ◽  
Cell Lines ◽  
Cell Monolayer ◽  
Low Frequency ◽  
Controlled Environment ◽  
Cell Nuclei ◽  
Extremely Low Frequency ◽  
The Impact

Abstract To assess the effects of exposure to extremely low-frequency magnetic fields (ELF-MFs) on MDCK cell lines, experiments were performed in a chamber under controlled conditions (temperature, humidity and CO2). Therefore, the measured physicochemical and electrical changes in the cells are due solely to the magnetic field exposure and not to external factors. A developed sinusoidal magnetic field generator produced the ELF-MFs with a uniform magnetic field and adjustable intensity and frequency. Three experimental indicators were used: (i) transepithelial electrical impedance (TEEI); (ii) cell migration and proliferation; and (iii) expression of the proteins of the tight junctions, and changes in the area and shape of the cell nuclei. No significant effects on TEEI values were observed when 10 and 50 G 60 Hz magnetic fields were applied to confluent cell monolayers. There were no significant differences in migration and proliferation of the cell monolayer exposed to 60 Hz magnetic fields10 and 50 G , but a contact inhibition factor was observed. The expression of the CLDN-1 protein decreased by 90% compared with the control, while ZO-1 protein expression increased by 120%. No significant effects were observed in the area and shape of the cell nuclei. Experimentation in a controlled environment, under physiological conditions, ensures that the observed effects were strictly due to exposure to magnetic fields. Different exposure conditions are necessary to determine the impact on TEEI and cell migration–proliferation indicators.

No evidence of DNA damage by co-exposure to extremely low frequency magnetic fields and aluminum on neuroblastoma cell lines

2017 ◽  
Vol 823 ◽  
pp. 11-21 ◽  
Author(s):  
Milena Villarini ◽  
Angela Gambelunghe ◽  
Daniela Giustarini ◽  
Maria Vittoria Ambrosini ◽  
Cristina Fatigoni ◽  
...  
Keyword(s):  
Dna Damage ◽  
Magnetic Fields ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Low Frequency ◽  
Extremely Low Frequency ◽  
Neuroblastoma Cell Lines

scholarly journals The impact of CLAUDIN-1 on follicular thyroid carcinoma aggressiveness

2015 ◽  
Vol 22 (5) ◽  
pp. 819-830 ◽  
Author(s):  
Denise Zwanziger ◽  
Julia Badziong ◽  
Saskia Ting ◽  
Lars Christian Moeller ◽  
Kurt Werner Schmid ◽  
...  
Keyword(s):  
Cell Migration ◽  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Follicular Thyroid Carcinoma ◽  
Regional Lymph Node ◽  
Migration And Invasion ◽  
Cell Nuclei ◽  
Membrane Expression ◽  
Cell Migration And Invasion ◽  
The Impact

CLAUDIN-1 belongs to the family of transmembrane tight junction proteins tightening the paracellular cleft of epithelial cells. In human malignancies, CLAUDIN-1 is often dysregulated and located in subcellular compartments, particularly in the nucleus where it may influence cellular behaviour. Here, we studied CLAUDIN-1 in relation to the biological characteristics of follicular thyroid carcinoma (FTC). CLAUDIN-1 immuno-staining showed loss of membrane expression and increased nuclear CLAUDIN-1 localization in FTC metastases. CLAUDIN-1 function was further investigated in two different follicular thyroid carcinoma cell lines: FTC-133 isolated from a regional lymph node metastasis and FTC-238 derived from a lung metastasis. In both cell lines CLAUDIN-1 expression was demonstrated in the cell nuclei with a significantly higher protein expression in FTC-238 compared to FTC-133 cells. Interestingly, in vitro scratch assay revealed enriched nuclear CLAUDIN-1 expression near the scratch. Furthermore, the increase of the pathogenic character of FTC-133 cells by RASV12 transfection was associated with elevated CLAUDIN-1 expression and enhanced cell migration, invasion and proliferation. Likewise over-expression of nuclear CLAUDIN-1 in FTC-133 cells resulted in increased cell migration and invasion. Conversely, CLAUDIN-1 downregulation in FTC-238 cells by siRNA resulted in decreased cell migration and invasion and was accompanied by reduced phosphoPKC expression. Moreover, activation and inhibition of PKC resulted in CLAUDIN-1 up- and downregulation in FTC cells respectively. These data suggest an impact of CLAUDIN-1 on follicular thyroid carcinoma aggressiveness, which could potentially be influenced by PKC activity.

Effects of weak low-frequency magnetic fields on spruce seed germination under acid conditions

1998 ◽  
Vol 28 (4) ◽  
pp. 609-616 ◽  
Author(s):  
Romana Ruzic ◽  
Igor Jerman ◽  
Nada Gogala
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Physiological Stress ◽  
Statistical Significance ◽  
Low Frequency ◽  
Magnetic Flux Density ◽  
Extremely Low Frequency ◽  
Sinusoidal Magnetic Field ◽  
Delayed Germination ◽  
Intensive Growth

We studied the effects of weak and extremely low frequency magnetic fields on the growth and germination of spruce seedlings (Picea abies (L.) Karst.) that were exposed simultaneously to different pH levels. The experiments showed that a weak, sinusoidal magnetic field (50 Hz, 26 and 105 µT, stimulation 12 h/day), which was computer controlled and generated by a system of Helmholtz-like coils, slightly reduced the length of the seedlings and delayed germination at low pH levels. A 26- µT magnetic field at 50 Hz slightly reduced the length of the seedlings at pH 2, delayed germination between pH 2 and 3, and caused no effects or were even stimulatory at pH 6 and 7. At a higher magnetic flux density (105 µT) the length, fresh weight, and germination were reduced mostly at pH 2 and 3 and with a higher statistical significance than at 26 µT. Germination was influenced during the first days of germination, and later the effect diminished rapidly. A 10- µT magnetic field at 46 Hz stimulated only the length of the seedlings. This phenomenon corresponds to the observations made by some other authors, namely that biological systems during various phases of intensive growth and (or) in suboptimal environmental conditions (physiological stress) are prone to exhibit greater sensitivity to extremely low frequency fields.

scholarly journals Measurement of the threshold sensitivity of honeybees to weak, extremely low-frequency magnetic fields

1997 ◽  
Vol 200 (9) ◽  
pp. 1363-1368 ◽  
Author(s):  
J Kirschvink ◽  
S Padmanabha ◽  
C Boyce ◽  
J Oglesby
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Low Frequency ◽  
Threshold Sensitivity ◽  
Extremely Low Frequency ◽  
Intensity Fluctuations ◽  
Field Strengths

Experiments reported previously demonstrate that free-flying honeybees are able to detect static intensity fluctuations as weak as 26 nT against the background, earth-strength magnetic field. We report here an extension of this work to weak, alternating fields at frequencies of 10 and 60 Hz. Our results indicate that the sensitivity of the honeybee magnetoreception system decreases rapidly with increasing frequency. At 60 Hz, alternating field strengths above 100 µT are required to elicit discrimination. These results are consistent with biophysical predictions of a magnetite-based magnetoreceptor.

The characteristics and present state for Extremely Low Frequency-Magnetic Fields of metropolitan subway

2015 ◽  
Vol 14 (1) ◽  
pp. 7-15
Author(s):  
Dae-kwan Jung ◽  
◽  
Joon-sig Jung ◽  
Kyu-mok Lee ◽  
Hyung-kyu Park ◽  
...  
Keyword(s):  
Magnetic Fields ◽  
Present State ◽  
Low Frequency ◽  
Extremely Low Frequency
Sign in / Sign up

Export Citation Format

Share Document