scholarly journals Validation of Microarray for the Simultaneous Detection of Common α- and β-Thalassemia Gene Mutations

2019 ◽  
Vol 50 (3) ◽  
pp. 306-312
Author(s):  
Sakorn Pornprasert ◽  
Rinradee Anurak ◽  
Chedtapak Ruengdit ◽  
Nattasit Pienthai ◽  
Monthathip Tookjai ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Gene Mutations ◽  
Accurate Method ◽  
Asian Countries ◽  
Medical Sciences ◽  
Chiang Mai ◽  
Genetic Characteristics ◽  
Hemoglobin E ◽  
Reference Methods ◽  
Southeast Asian Countries

Abstract Background Methods for detecting the complex genetic characteristics of α- and β-thalassemias are required for preventing and controlling the outbreak of new cases. Methods We evaluated the accuracy and practical utility of microarray for simultaneous detection of α- and β-thalassemias. A total of 102 DNA specimens, which represented 25 different genotypes, were tested in parallel using the microarray and reference methods used in the thalassemia laboratory of the Associated Medical Sciences–Clinical Services Center (AMS-CSC), Chiang Mai, Thailand. Results A total of 100 (98.0%) DNA specimens were completely concordant between the microarray and reference methods, whereas discrepancies between the different methods were observed in only 2 DNA specimens with homozygous hemoglobin E (HbE). Conclusions The microarray appeared to be a fast, easy to perform, and accurate method for simultaneous detection of α- and β-thalassemias in Thailand and Southeast Asian countries. However, this technique needs to be improved and validated in a larger number of specimens with homozygous HbE before further routine laboratory use.

Proficiency testing program for hemoglobin E, A2 and F analysis in Thailand using lyophilized hemoglobin control materials

2018 ◽  
Vol 56 (4) ◽  
pp. 602-608 ◽  
Author(s):  
Sakorn Pornprasert ◽  
Monthathip Tookjai ◽  
Manoo Punyamung ◽  
Panida Pongpunyayuen ◽  
Kallayanee Treesuwan
Keyword(s):  
Proficiency Testing ◽  
Asian Population ◽  
Medical Sciences ◽  
Chiang Mai ◽  
Hemoglobin E ◽  
Laboratory Staff ◽  
Study Results ◽  
Different Types ◽  
Thalassemia Trait ◽  
Materials Used

Abstract Background: There is no external quality assessment (EQA) program for hemoglobin analysis that uses lyophilized hemoglobin control materials with HbA2/E in levels as high as those found in people with the β-thalassemia trait, HbE trait, β-thalassemia/HbE disease or homozygous HbE; these are all found frequently in the southeast Asian population. The aim of this study was to evaluate the efficiency of the control materials used in the established proficiency testing (PT) program at the Associated Medical Sciences-Clinical Service Center (AMC-CSC), Chiang Mai University, Chiang Mai, Thailand. Methods: The PT program for Hb analysis and the thalassemia interpretation was established in compliance with ISO/IEC17043:2010. Three cycles per year were performed in 2015 and 2016. In each cycle, three different types of control material were provided to the participants. Each participant analyzed the control materials in the same manner as in their routine practices. Hb analysis results and their thalassemia interpretation codes were entered into the report form and sent back to AMC-CSC. Results: The number of participants increased from 63 in 2015 to 76 in 2016. In addition, the number of participants who took part in all three cycles increased from 95.2% (60/63) in 2015 to 100% (76/76) in 2016. All participants reported the correct Hb measurement and type; however, misinterpretations in thalassemia diagnosis were noted. Conclusions: The lyophilized hemoglobin control materials prepared at AMC-CSC were used successfully in our PT program. However, the study results indicate the need for further improvement in thalassemia interpretation skills for laboratory staff.

scholarly journals Relevance of Sampling Techniques in Medical Research

2015 ◽  
Vol 2 (1) ◽  
pp. 3-6
Author(s):  
Brijesh Sathian ◽  
Jayadevan Sreedharan ◽  
Bedanta Roy ◽  
Indrajit Banerjee ◽  
Hosuru Subramanya Supram
Keyword(s):  
Medical Research ◽  
Medical Practice ◽  
Sampling Methods ◽  
Southeast Asian ◽  
Sampling Techniques ◽  
Asian Countries ◽  
Biomedical Sciences ◽  
Medical Sciences ◽  
Relevant Research ◽  
Southeast Asian Countries

It is the era of developments and technologies in Southeast Asian countries including Nepal. Medical practice is growing to its supreme level. There is several funded and clinically relevant research works from this region. But it is questionable whether the data is collected scientifically and the inferred evidence is useful to the medical sciences. Improper sampling methods will result with biased evidence, which will make the researcher misleading. This article will discuss the basic sampling techniques required for a good research.DOI: http://dx.doi.org/10.3126/jbs.v2i1.13031Journal of Biomedical Sciences 2015 Mar.; 2(1): 3-6

Malaysian Homestays: The Need for Standardized Regulation

2020 ◽  
Vol 17 (2) ◽  
pp. 63
Author(s):  
ROHASLINDA BINTI RAMELE ◽  
YAMAZAKI JUICHI ◽  
MD NAJIB IBRAHIM ◽  
LILIS SHEREENA SAFIEE
Keyword(s):  
Rural Community ◽  
Rural Tourism ◽  
Asian Countries ◽  
Government Organizations ◽  
Rural Income ◽  
Arts And Culture ◽  
The Government ◽  
Host Families ◽  
Southeast Asian Countries ◽  
Management Operation

This study aims to clarify regulations used among each type of registered and unregistered Malaysian homestays and to reveal issues arose regarding the implemented regulations. Selected homestays that are established by the government organizations and individuals were investigated to carry out the comparison on each homestay. Host families and persons in charge of the Ministry of Tourism, Arts and Culture (MOTAC), the Ministry of Agriculture (MOA), and the Ministry of Rural and Regional Development (MRRD) were also interviewed. Findings showed that there is only one guideline used for all types of homestays in Malaysia, which is the Malaysia Homestay Registration Guideline, established by MOTAC. MOTAC has also been selected as a leader of homestays in the Southeast Asian countries by the ASEAN Secretariat; therefore, the ASEAN Homestay Standard is also being referred. However, the implementation of this guideline and standard among other homestays unregistered with MOTAC (homestays established by MOA, MRRD, and individuals) is not compulsory, although encouraged, where they may receive equal benefits in term of facilities and promotion. Some issues arose due to the tax regulated by the local authorities, failure on homestays to be registered with MOTAC, and abandoned homestays. This study recommended that the Malaysia Homestay Registration Guideline to be standardized and regulated to all types of homestays, including individuals and unregistered homestays in Malaysia. This is important in order to reduce issues arose involving the homestay industry, providing safety and comfort to the tourists and, to help to develop rural income among host families and the rural community themselves.Keywords: Rural tourism, homestay, regulation, management, operation

Factors Affecting Bank Risk-Taking: Evidence from Southeast Asian Countries

2016 ◽  
Vol 23 (2) ◽  
pp. 120-136
Author(s):  
NGUYEN THANH LIEM ◽  
TRAN HUNG SON ◽  
HOANG TRUNG NGHIA
Keyword(s):  
Risk Taking ◽  
Southeast Asian ◽  
Bank Risk ◽  
Asian Countries ◽  
Factors Affecting ◽  
Southeast Asian Countries

Transboundary Haze-Free for Southeast Asian Countries by 2020: A Delusional Vision?

2020 ◽  
Vol 24 (02) ◽  
pp. 1923-1929
Author(s):  
Nurhidayatuloh ◽  
Febrian ◽  
Mada Apriandi ◽  
Annalisa Y ◽  
Helena Primadianti Sulistyaningrum ◽  
...  
Keyword(s):  
Southeast Asian ◽  
Asian Countries ◽  
Southeast Asian Countries

scholarly journals How do Southeast Asian countries participate in the development of the Arctic

2021 ◽  
Vol 625 ◽  
pp. 012009
Author(s):  
E E Krasnozhenova ◽  
S V Kulik ◽  
T Chistalyova ◽  
K Yu Eidemiller ◽  
P L Karabushenko
Keyword(s):  
Southeast Asian ◽  
The Arctic ◽  
Asian Countries ◽  
Southeast Asian Countries

scholarly journals Description of Echolocation Call Parameters for Urban Bats in Vietnam as a Step Towards a More Integrated Acoustic Monitoring of Urban Wildlife in Southeast Asia

Diversity ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 18
Author(s):  
Long Kim Pham ◽  
Bang Van Tran ◽  
Quy Tan Le ◽  
Trung Thanh Nguyen ◽  
Christian C. Voigt
Keyword(s):  
Discriminant Function ◽  
Discriminant Function Analysis ◽  
Function Analysis ◽  
Quantitative Description ◽  
Southeast Asian ◽  
Asian Countries ◽  
Echolocation Call ◽  
Echolocation Calls ◽  
Pilot Work ◽  
Southeast Asian Countries

This study is the first step towards more systematic monitoring of urban bat fauna in Vietnam and other Southeast Asian countries by collecting bat echolocation call parameters in Ho Chi Minh and Tra Vinh cities. We captured urban bats and then recorded echolocation calls after releasing in a tent. Additional bat’s echolocation calls from the free-flying bats were recorded at the site where we captured bat. We used the obtained echolocation call parameters for a discriminant function analysis to test the accuracy of classifying these species based on their echolocation call parameters. Data from this pilot work revealed a low level of diversity for the studied bat assemblages. Additionally, the discriminant function analysis successfully classified bats to four bat species with an accuracy of >87.4%. On average, species assignments were correct for all calls from Taphozous melanopogon (100% success rate), for 70% of calls from Pipistrellus javanicus, for 80.8% of calls from Myotis hasseltii and 67.3% of calls from Scotophilus kuhlii. Our study comprises the first quantitative description of echolocation call parameters for urban bats of Vietnam. The success in classifying urban bats based on their echolocation call parameters provides a promising baseline for monitoring the effect of urbanization on bat assemblages in Vietnam and potentially also other Southeast Asian countries.

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