New multilocus sequence typing primers to enable genotyping of AD hybrids within the Cryptococcus neoformans species complex

2020 ◽  
Vol 58 (7) ◽  
pp. 1005-1009
Author(s):  
Massimo Cogliati ◽  
Frederic Roger ◽  
Wieland Meyer ◽  
Vincent Robert ◽  
Sébastien Bertout
Keyword(s):  
Molecular Epidemiology ◽  
Cryptococcus Neoformans ◽  
Multilocus Sequence Typing ◽  
Species Complex ◽  
Mlst Database ◽  
Specific Primers ◽  
Typing Method ◽  
Genetic Studies ◽  
Epidemiology Studies ◽  
Ad Hybrids

Abstract   Although AD hybrids within the Cryptococcus neoformans species complex represent about 20% of the isolates identified in Europe, phylogenetic and population genetic studies are lacking due to the inability to use the standardized typing method. The aim of the present study was to design new molecular type specific primers in order to apply the standard ISHAM consensus multilocus sequence typing (MLST) scheme to AD hybrids. The new primers are able to specifically amplify VNI and VNIV alleles of the seven MLST loci in both haploid and diploid or aneuploid hybrid strains. This study forms the basis for future molecular epidemiology studies of AD hybrids. Lay abstract We designed and tested new specific primers to amplify the two alleles of each of the seven MLST loci in C. neoformans species complex hybrids. The sequences obtained from hybrids can be compared with those present in the Cryptococcus global MLST database for future molecular epidemiology studies.

scholarly journals Molecular Epidemiology Reveals Genetic Diversity amongst Isolates of the Cryptococcus neoformans/C. gattii Species Complex in Thailand

2013 ◽  
Vol 7 (7) ◽  
pp. e2297 ◽  
Author(s):  
Sirada Kaocharoen ◽  
Popchai Ngamskulrungroj ◽  
Carolina Firacative ◽  
Luciana Trilles ◽  
Dumrongdej Piyabongkarn ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Epidemiology ◽  
Cryptococcus Neoformans ◽  
Species Complex

Molecular epidemiology of isolates of the Cryptococcus neoformans species complex from Spain

2009 ◽  
Vol 26 (2) ◽  
pp. 112-117 ◽  
Author(s):  
Susana Frasés ◽  
Consuelo Ferrer ◽  
Manuel Sánchez ◽  
María Francisca Colom-Valiente
Keyword(s):  
Molecular Epidemiology ◽  
Cryptococcus Neoformans ◽  
Species Complex

scholarly journals Molecular epidemiology and antifungal susceptibility profiles of clinical Cryptococcus neoformans/Cryptococcus gattii species complex

2020 ◽  
Vol 69 (1) ◽  
pp. 72-81 ◽  
Author(s):  
Zainab Bandalizadeh ◽  
Tahereh Shokohi ◽  
Hamid Badali ◽  
Mahdi Abastabar ◽  
Farhang Babamahmoudi ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Cryptococcus Neoformans ◽  
Species Complex ◽  
Antifungal Susceptibility ◽  
Cryptococcus Gattii ◽  
Susceptibility Profiles

scholarly journals Environmental Status of Cryptococcus neoformans and Cryptococcus gattii in Colombia

2021 ◽  
Vol 7 (6) ◽  
pp. 410
Author(s):  
Briggith-Nathalia Serna-Espinosa ◽  
Diomedes Guzmán-Sanabria ◽  
Maribel Forero-Castro ◽  
Patricia Escandón ◽  
Zilpa Adriana Sánchez-Quitian
Keyword(s):  
Cryptococcus Neoformans ◽  
Species Complex ◽  
Columba Livia ◽  
Systematic Investigation ◽  
Cryptococcus Gattii ◽  
Eucalyptus Species ◽  
Environmental Niche ◽  
Worldwide Distribution ◽  
Bird Droppings ◽  
The Central Nervous System

The genus Cryptococcus comprises more than 80 species, including C. neoformans and C. gattii, which are pathogenic to humans, mainly affecting the central nervous system. The two species differ in geographic distribution and environmental niche. C. neoformans has a worldwide distribution and is often isolated from bird droppings. On the contrary, C. gattii is reported in tropical and subtropical regions and is associated with Eucalyptus species. This review aims to describe the distribution of environmental isolates of the Cryptococcus neoformans species complex and the Cryptococcus gattii species complex in Colombia. A systematic investigation was carried out using different databases, excluding studies of clinical isolates reported in the country. The complex of the species of C. gattii is recovered mainly from trees of the genus Eucalyptus spp., while the complex of the species of C. neoformans is recovered mainly from avian excrement, primarily Columba livia (pigeons) excrement. In addition, greater positivity was found at high levels of relative humidity. Likewise, an association was observed between the presence of the fungus in places with little insolation and cold or temperate temperatures compared to regions with high temperatures.

scholarly journals 32P-Post-labelling method improvements for aromatic compound-related molecular epidemiology studies

Mutagenesis ◽  
2007 ◽  
Vol 22 (6) ◽  
pp. 381-385 ◽  
Author(s):  
A. Munnia ◽  
F. Saletta ◽  
A. Allione ◽  
S. Piro ◽  
M. Confortini ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Aromatic Compound ◽  
Epidemiology Studies ◽  
Labelling Method

Multilocus sequence typing of strains from the Cryptococcus gattii species complex from different continents

Mycoses ◽  
2021 ◽  
Author(s):  
Bellet Virginie ◽  
Roger Frédéric ◽  
Krasteva Donika ◽  
Gouveia Tiphany ◽  
Drakulovski Pascal ◽  
...  
Keyword(s):  
Multilocus Sequence Typing ◽  
Species Complex ◽  
Cryptococcus Gattii

scholarly journals Multilocus sequence typing of Cryptococcus neoformans var. grubii from Laos in a regional and global context

2018 ◽  
Vol 57 (5) ◽  
pp. 557-565 ◽  
Author(s):  
Lam Tuan Thanh ◽  
Trieu Hai Phan ◽  
Sayaphet Rattanavong ◽  
Trinh Mai Nguyen ◽  
Anh Van Duong ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Multilocus Sequence Typing ◽  
Global Context
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