scholarly journals Corrigendum: Increased virulence of albino mutant of Fonsecaea monophora in Galleria mellonella

2019 ◽  
Vol 57 (8) ◽  
pp. e13-e13
Author(s):  
Yinghui Liu ◽  
Xiaowen Huang ◽  
Hongfang Liu ◽  
Liyan Xi ◽  
Chester R Cooper
Keyword(s):  
Galleria Mellonella ◽  
Fonsecaea Monophora ◽  
Albino Mutant

Increased virulence of albino mutant of Fonsecaea monophora in Galleria mellonella

2019 ◽  
Vol 57 (8) ◽  
pp. 1018-1023 ◽  
Author(s):  
Yinghui Liu ◽  
Xiaowen Huang ◽  
Hongfang Liu ◽  
Liyan Xi ◽  
Chester R Cooper
Keyword(s):  
Immune Response ◽  
Galleria Mellonella ◽  
Southern China ◽  
Immunological Mechanism ◽  
Fonsecaea Monophora ◽  
Albino Mutant ◽  
Predominant Pathogen ◽  
Cellular Immune ◽  
Albino Strain

Abstract Fonsecaea monophora has been the predominant pathogen of chromoblastomycosis in Southern China, but its pathogenic mechanism remains unclear. New models are needed to study this infection. In the current study, we examined the role of melanin on the pathogenicity of F. monophora in Galleria mellonella model using melanin and albino strain. Interestingly, the albino mutant strain displayed higher pathogenicity compared to the melanin stain and restoration of melanin of albino mutant could reverse the pathogenicity. Histopathology showed that inflammatory nodules were bigger than that infected with albino cells, which suggested that melanized cells could trigger a robust cellular immune response of G. mellonella than albino cells. The activated immune response in G. mellonella induced by melanized cells might explain the decreased virulence of melanized cells in larvae model. While further study was needed to gain full insights into the molecular immunological mechanism in G. mellonella activated by melanin.

Die Raupe Galleria mellonella als Infektionsmodell

2017 ◽  
Author(s):  
M Olsowski ◽  
J Steinmann ◽  
D Theegarten
Keyword(s):  
Galleria Mellonella

INFLUÊNCIA DO SUBSTRATO E DA LUMINOSIDADE NA INFECÇÃO DE NEMATOIDES ENTOMOPATOGÊNICOS EM Galleria mellonella (LEPIDOPTERA: PYRALIDAE)

2018 ◽  
Vol 20 (2) ◽  
pp. 91-101
Author(s):  
Andressa Lima de Brida ◽  
Silvia Renata Siciliano Wilcken ◽  
Luis Garrigós Leite
Keyword(s):  
Galleria Mellonella ◽  
Steinernema Carpocapsae ◽  
Steinernema Feltiae ◽  
Lepidoptera Pyralidae

Nematoides entomopatogênicos (NEPs) são alternativas eficientes para o controle de pragas. O emprego de novas técnicas da produção in vivo, permite o progresso da tecnologia de formulação de bioinseticidas. O objetivo do trabalho, foi avaliar a influência da luminosidade e do substrato na capacidade de infecção de juvenis infectantes (JIs) de Steinernema brazilense IBCBn 06, Steinernema carpocapsae IBCBn 02, Steinernema feltiae IBCBn 47 e Heterorhabditis amazonensis IBCBn 24 em lagartas de Galleria mellonella (Lepidoptera: Pyralidae). O delineamento experimental foi inteiramente casualizado com quatro tratamentos e oito repetições. As parcelas, constituídas por placa de Petri com, substrato-areia e substrato-papel filtro, com e sem luminosidade, inoculados com suspensão de 1,5 mL contendo 400JIs e quatro lagartas de G. mellonella. O número de JIs foi quantificado após a mortalidade das lagartas. A taxa de infecção de JIs de S. carpocapsae IBCBn 02 e S. feltiae IBCBn 47 variaram de 2,14 a 3,28 e de 11,04 a 13,09 JIs/lagarta. O substrato-areia com e sem luminosidade permitiu a maior taxa de infeção dos JIs de S. brazilense IBCBn 06 de 7,86 e 9,44 JIs/lagarta, e 13,49 JIs/lagarta com luminosidade para H. amazonensis IBCBn 24. O substrato-areia, permite a maior taxa de infecção por JIs de NEPs.

scholarly journals A Peptide Found in Human Serum, Derived from the C-Terminus of Albumin, Shows Antifungal Activity In Vitro and In Vivo

2020 ◽  
Vol 8 (10) ◽  
pp. 1627
Author(s):  
Tecla Ciociola ◽  
Pier Paolo Zanello ◽  
Tiziana D’Adda ◽  
Serena Galati ◽  
Stefania Conti ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Human Serum ◽  
Fungicidal Activity ◽  
Galleria Mellonella ◽  
Serum Proteins ◽  
Morphological Alterations ◽  
C Terminus ◽  
Different Sources

The growing problem of antimicrobial resistance highlights the need for alternative strategies to combat infections. From this perspective, there is a considerable interest in natural molecules obtained from different sources, which are shown to be active against microorganisms, either alone or in association with conventional drugs. In this paper, peptides with the same sequence of fragments, found in human serum, derived from physiological proteins, were evaluated for their antifungal activity. A 13-residue peptide, representing the 597–609 fragment within the albumin C-terminus, was proved to exert a fungicidal activity in vitro against pathogenic yeasts and a therapeutic effect in vivo in the experimental model of candidal infection in Galleria mellonella. Studies by confocal microscopy and transmission and scanning electron microscopy demonstrated that the peptide penetrates and accumulates in Candida albicans cells, causing gross morphological alterations in cellular structure. These findings add albumin to the group of proteins, which already includes hemoglobin and antibodies, that could give rise to cryptic antimicrobial fragments, and could suggest their role in anti-infective homeostasis. The study of bioactive fragments from serum proteins could open interesting perspectives for the development of new antimicrobial molecules derived by natural sources.

scholarly journals Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 819
Author(s):  
Nicolai Rügen ◽  
Timothy P. Jenkins ◽  
Natalie Wielsch ◽  
Heiko Vogel ◽  
Benjamin-Florian Hempel ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Galleria Mellonella ◽  
Systemic Reactions ◽  
Venom Composition ◽  
Assassin Bug ◽  
Molecular Components ◽  
First Time ◽  
Tissue Digestion

Assassin bug venoms are potent and exert diverse biological functions, making them potential biomedical goldmines. Besides feeding functions on arthropods, assassin bugs also use their venom for defense purposes causing localized and systemic reactions in vertebrates. However, assassin bug venoms remain poorly characterized. We collected the venom from the assassin bug Rhynocoris iracundus and investigated its composition and bioactivity in vitro and in vivo. It caused lysis of murine neuroblastoma, hepatoma cells, and healthy murine myoblasts. We demonstrated, for the first time, that assassin bug venom induces neurolysis and suggest that it counteracts paralysis locally via the destruction of neural networks, contributing to tissue digestion. Furthermore, the venom caused paralysis and melanization of Galleria mellonella larvae and pupae, whilst also possessing specific antibacterial activity against Escherichia coli, but not Listeria grayi and Pseudomonas aeruginosa. A combinatorial proteo-transcriptomic approach was performed to identify potential toxins responsible for the observed effects. We identified neurotoxic Ptu1, an inhibitory cystin knot (ICK) toxin homologous to ω-conotoxins from cone snails, cytolytic redulysins homologous to trialysins from hematophagous kissing bugs, and pore-forming hemolysins. Additionally, chitinases and kininogens were found and may be responsible for insecticidal and cytolytic activities. We demonstrate the multifunctionality and complexity of assassin bug venom, which renders its molecular components interesting for potential biomedical applications.

scholarly journals In Silico Predicted Antifungal Peptides: In Vitro and In Vivo Anti-Candida Activity

2021 ◽  
Vol 7 (6) ◽  
pp. 439
Author(s):  
Tecla Ciociola ◽  
Walter Magliani ◽  
Tiziano De Simone ◽  
Thelma A. Pertinhez ◽  
Stefania Conti ◽  
...  
Keyword(s):  
In Silico ◽  
Mammalian Cells ◽  
Galleria Mellonella ◽  
Ex Vivo ◽  
Consensus Sequence ◽  
In Silico Analysis ◽  
Significant Activity ◽  
Synthetic Antibody

It has been previously demonstrated that synthetic antibody-derived peptides could exert a significant activity in vitro, ex vivo, and/or in vivo against microorganisms and viruses, as well as immunomodulatory effects through the activation of immune cells. Based on the sequence of previously described antibody-derived peptides with recognized antifungal activity, an in silico analysis was conducted to identify novel antifungal candidates. The present study analyzed the candidacidal and structural properties of in silico designed peptides (ISDPs) derived by amino acid substitutions of the parent peptide KKVTMTCSAS. ISDPs proved to be more active in vitro than the parent peptide and all proved to be therapeutic in Galleria mellonella candidal infection, without showing toxic effects on mammalian cells. ISDPs were studied by circular dichroism spectroscopy, demonstrating different structural organization. These results allowed to validate a consensus sequence for the parent peptide KKVTMTCSAS that may be useful in the development of novel antimicrobial molecules.

scholarly journals Defective phagocyte association during infection of Galleria mellonella with Yersinia pseudotuberculosis is detrimental to both insect host and microbe

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 638-653
Author(s):  
Anne Marie Krachler ◽  
Natalie Sirisaengtaksin ◽  
Pauline Monteith ◽  
C. E. Timothy Paine ◽  
Christopher J. Coates ◽  
...  
Keyword(s):  
Yersinia Pseudotuberculosis ◽  
Galleria Mellonella ◽  
Insect Host
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