INFLUENCE OF THE QUINOLIZIDINE ALKALOID CONTENT OF LUPINUS ALBUS (FABACEAE) ON THE FEEDING CHOICE OF HELIX ASPERSA (GASTROPODA: PULMONATA)

2000 ◽  
Vol 66 (1) ◽  
pp. 61-68 ◽  
Author(s):  
L. CHEVALIER
Keyword(s):  
Lupinus Albus ◽  
Helix Aspersa ◽  
Alkaloid Content ◽  
Quinolizidine Alkaloid ◽  
Feeding Choice

scholarly journals COLORIMETRIC VS. CHROMATOGRAPHIC ANALYSES OF ALKALOIDS IN LUPIN SEEDS

2018 ◽  
Vol 78 ◽  
pp. 63-67
Author(s):  
Paulina Wilczura ◽  
Wojciech Święcicki ◽  
Katarzyna Kamel ◽  
Wiesław Wasiak
Keyword(s):  
Bitter Taste ◽  
Lupinus Albus ◽  
Colorimetric Method ◽  
Dry Weight ◽  
Alkaloid Content ◽  
Estimation Methods ◽  
Material Development ◽  
Colorimetric Test ◽  
Lupin Seeds ◽  
Accurate Quantification

A characteristic trait of lupins is a production of alkaloids, which are a toxic and bitter taste compound ofseeds. Due to the lack of fast, sensitive and inexpensive screening techniques to identify and reject high alkaloidplant material, development of suitable tools is important challenges for lupins breeding and seed production.The aim of this study was to compare two alkaloid content estimation methods in Lupinus angustifoliusL. and Lupinus albus L.During the Wagner’s colorimetric test, which is recommended by the UPOV, seed halves were stained onfour colors depending on the alkaloid content but only the level of 0.5% – 0.6% showed clear color change.Gas chromatography allowed accurate quantification and qualification of alkaloid content.Since safe alkaloid content for consumption is 0.02% of seed dry weight, colorimetric method is less usefulfor dividing lupin cultivars into sweet and bitter, than gas chromatography but can be used as a screeningtechnique.

Interrelationship between Quinolizidine Alkaloid Producing Legumes and Infesting Insects: Exploitation of the Alkaloid- Containing Phloem Sap of Cytisus scoparius by the Broom Aphid Aphis cytisorum

1982 ◽  
Vol 37 (11-12) ◽  
pp. 1081-1086 ◽  
Author(s):  
Michael Wink ◽  
Thomas Hartmann ◽  
Ludger Witte ◽  
Joachim Rheinheimer
Keyword(s):  
Direct Evidence ◽  
Xylem Sap ◽  
Aphid Resistance ◽  
Alkaloid Content ◽  
Quinolizidine Alkaloids ◽  
Cytisus Scoparius ◽  
Aphid Infestation ◽  
Phloem Sap ◽  
Quinolizidine Alkaloid ◽  
Evidence Analysis

Abstract Aphids (Aphis cytisorum) which infest broom plants (Cytisus scoparius) accumulate up to 500 μg alkaloid/g fr. wt. The alkaloids, which are similar to those of the plants, consist of 17-oxo-sparteine, sparteine, 12,13-dehydrosparteine, and lupanine. Infested plants contain > 50% less alkaloids than aphid-free plants. In Lupinus aphid resistance which is due to their high alkaloid content is more expressed: Whereas bitter varieties are free from aphids, only the sweet alkaloid-free plants are susceptible to aphid infestation. The accumulation of alkaloids in aphids indicates that the quinolizidine alkaloids are translocated via the phloem in legume plants. This assumption is supported by direct evidence: analysis of phloem sap from Lupinus contains up to 5 mg alkaloid whereas xylem sap is virtually free of alkaloids. The interrelationship between quinolizidine alkaloids and hervivores is discussed.

Treatment of Lupin Seed Using the Instantaneous Controlled Pressure Drop Technology to Reduce Alkaloid Content

2006 ◽  
Vol 12 (5) ◽  
pp. 365-370 ◽  
Author(s):  
J. Haddad ◽  
M. Muzquiz ◽  
K. Allaf
Keyword(s):  
Pressure Drop ◽  
Total Alkaloid ◽  
Lupinus Albus ◽  
Grain Legumes ◽  
Water Soluble ◽  
Alkaloid Content ◽  
High Protein Content ◽  
Lupinus Mutabilis ◽  
Lupin Seeds ◽  
Total Alkaloid Content

Despite their high protein content, lupin seeds are not as fully utilised as some other grain legumes. The drawback in their utilisation is mainly due to the presence of water soluble, poisonous alkaloids. In this study, an instantaneous controlled pressure drop (DIC) treatment followed by an aqueous extraction was performed on lupin seeds. Two species Lupinus albus and Lupinus mutabilis with different initial total alkaloid content of 0.025% and 5.5% (d.b.), respectively have been studied. Lupanine was the major alkaloid found in both lupin species. Experimental results proved the feasibility of using DIC treatment to reduce the alkaloid content of lupin seeds. Optimised DIC treatment combined with an adequate 2h soaking in water reduced the alkaloid content from 5.5% to 2.2% of L. mutabilis and from 0.025% to 0.0075% of L. albus.

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