A nonanucleotide sequence involved in promotion of ribosomal RNA synthesis and RNA priming of DNA replication on yeast mitochondria

Ribosomal RNA is synthesized at constant rate during most of the cell cycle in heat-shock synchronized populations of Tetrahymena pyriformis. Early in each macronuclear S-period the rate of synthesis increases abruptly, concomitant with replication of the genes coding for ribosomal RNA. The increase is prevented by inhibitors of DNA replication, added prior to the S-period. Similarly, in cultures synchronized by starvation/refeeding, inhibition of DNA replication, at the time when the rDNA is replicated, will prevent the normal increase in rate of RNA synthesis which follows refeeding. We conclude that inhibition of rDNA replication interferes with the synthesis of rRNA, and we suggest that with respect to rRNA synthesis a gene dosis effect is operating in fast-growing Tetrahymena cells.

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Inhibition of rRNA synthesis following incorporation of 5-bromodeoxyuridine into DNA of Tetrahymena pyriformis

Journal of Cell Science ◽

10.1242/jcs.17.3.495 ◽

1975 ◽

Vol 17 (3) ◽

pp. 495-502

Author(s):

A.E. Lykkesfeldt ◽

H.A. Andersen

Keyword(s):

Dna Replication ◽

Ribosomal Rna ◽

Rna Synthesis ◽

Nuclear Dna ◽

Tetrahymena Pyriformis ◽

Growth Conditions ◽

Defined Medium ◽

Rrna Synthesis ◽

Chemically Defined Medium ◽

Dividing Cells

Tetrahymena pyriformis was grown on chemically defined medium in the presence of 5-bromodeoxyuridine (BUdR). Under these growth conditions more than 60% of the thymidine sites in DNA were substituted with BUdR. It was found that RNA synthesis was strongly inhibited by the presence of BUdR in DNA. To assure that incorporation of BUdR into DNA was a prerequisite of the effect observed, BUdR was added to synchronously dividing cells. BUdR had no effect on the cells when present outside the period of nuclear DNA replication, whereas RNA synthesis was strongly inhibited as soon as the genes coding for ribosomal RNA had replicated in the presence of BUdR.

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Faculty Opinions recommendation of Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1024764.293740 ◽

2005 ◽

Author(s):

Lucio Comai

Keyword(s):

Ribosomal Rna ◽

Rna Synthesis ◽

Drosophila Development

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Faculty Opinions recommendation of A Molecular Titration System Coordinates Ribosomal Protein Gene Transcription with Ribosomal RNA Synthesis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726937139.793528937 ◽

2017 ◽

Author(s):

Catherine Fox

Keyword(s):

Ribosomal Protein ◽

Gene Transcription ◽

Ribosomal Rna ◽

Rna Synthesis ◽

Protein Gene ◽

Ribosomal Protein Gene ◽

Titration System ◽

Molecular Titration

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Accelerated rates of ribosomal RNA synthesis during growth of contracting heart cells in culture

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)84700-2 ◽

1989 ◽

Vol 264 (30) ◽

pp. 18220-18227

Author(s):

P J McDermott ◽

L I Rothblum ◽

S D Smith ◽

H E Morgan

Keyword(s):

Ribosomal Rna ◽

Rna Synthesis ◽

Heart Cells ◽

Cells In Culture ◽

Contracting Heart

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DIFFERENTIATION OF DROSOPHILA CELLS LACKING RIBOSOMAL DNA, IN VITRO

Genetics ◽

10.1093/genetics/73.3.429 ◽

1973 ◽

Vol 73 (3) ◽

pp. 429-434

Author(s):

J James Donady ◽

R L Seecof ◽

M A Fox

Keyword(s):

Drosophila Melanogaster ◽

Ribosomal Rna ◽

Ribosomal Dna ◽

Rna Synthesis ◽

Wild Type ◽

Drosophila Cells

ABSTRACT Drosophila melanogaster embryos that lacked ribosomal DNA were obtained from appropriate crosses. Cells were taken from such embryos before overt differentiation took place and were cultured in vitro. These cells differentiated into neurons and myocytes with the same success as did wild-type controls. Therefore, ribosomal RNA synthesis is not necessary for the differentiation of neurons and myocytes in vitro.

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