scholarly journals Generation of nonradioactive bromodeoxyuridine labeled DNA probes by polymerase chain reaction

1991 ◽  
Vol 19 (10) ◽  
pp. 2783-2783 ◽  
Author(s):  
S. Tabibzadeh ◽  
U.G. Bhat ◽  
X. Sun
Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 313-322 ◽  
Author(s):  
P. A. O. Majiwa ◽  
R. Thatthi ◽  
S. K. Moloo ◽  
J. H. P. Nyeko ◽  
L. H. Otieno ◽  
...  

SUMMARYRelatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes.


1996 ◽  
Vol 70 (10) ◽  
pp. 1072-1078
Author(s):  
Keiko YAMASHITA ◽  
Shohei KAGAWA ◽  
Akira MATSUOKA ◽  
Yutaka TAKARADA

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