scholarly journals Campylobacter jejuni: Specific oligonucleotides and DNA probes for use in polymerase chain reaction-based diagnosis

1993 ◽  
Vol 7 (4) ◽  
pp. 337-344 ◽  
Author(s):  
V�ronique Stonnet ◽  
Jean-Luc Guesdon
Keyword(s):  
Polymerase Chain Reaction ◽  
Campylobacter Jejuni ◽  
Dna Probes ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of trypanosome infections in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle

Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 313-322 ◽  
Author(s):  
P. A. O. Majiwa ◽  
R. Thatthi ◽  
S. K. Moloo ◽  
J. H. P. Nyeko ◽  
L. H. Otieno ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Buffy Coat ◽  
Dna Probes ◽  
Tsetse Flies ◽  
Chain Reaction ◽  
African Trypanosomes ◽  
Polymerase Chain

SUMMARYRelatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes.

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