scholarly journals BIOM-12. CIRCULATING TUMOR DNA IN ADULTS WITH GLIOMA: A SYSTEMATIC REVIEW AND META-ANALYSIS OF BIOMARKER SENSITIVITY

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii3-ii4
Author(s):  
James McMahon ◽  
Matthew Studer ◽  
Bryan Ulrich ◽  
Gustavo Pradilla
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Circulating Tumor Dna ◽  
Low Grade ◽  
High Grade ◽  
Who Grade ◽  
Targeted Next Generation Sequencing ◽  
Non Invasive ◽  
Performance Statistics ◽  
Tumor Dna

Abstract BACKGROUND Circulating tumor DNA (ctDNA) has emerged as a promising non-invasive biomarker to capture tumor genetics in patients with primary brain tumors. Research into its clinical utility, however, has not been standardized, as performance statistics of ctDNA remain undefined and optimal ctDNA assay and biospecimen sources for its evaluation have not been conclusively identified. We sought to determine a pooled sensitivity of the detection ctDNA in both CSF in plasma when compared to detecting the same mutant DNA in tumor tissue of gliomas. We then sought to compare ctDNA sensitivity between these two reservoirs, as well as between individual WHO grades of glioma. METHODS Following PRISMA guidelines, systematic review and meta-analysis was performed using published studies that assessed circulating tumor DNA in either plasma or CSF among adult patients with histopathology-confirmed glioma. Weighting of individual studies was conducted to reach an overall pooled sensitivity of ctDNA detection in both CSF and plasma. Chi-squared tests of independence were performed to compare overall sensitivity of ctDNA in CSF versus plasma, as well as to estimate the sensitivity of ctDNA for each WHO grade of glioma. RESULTS The overall reported sensitivity of ctDNA in CSF was found to be 77.4%, significantly higher than the 38.8% sensitivity in plasma (p< 0.0001). Sensitivity was significantly higher for high grade (82.8%) than low grade (60.5%) tumors in CSF (p=0.0023), and sensitivity was found to sequentially increase with increasing WHO grade. Qualitative analysis revealed evidence of greater sensitivity among single-allele PCR or small targeted next generation sequencing (NGS) panels, and increased sensitivity among larger tumors and those in proximity to cisternal or ventricular CSF. CONCLUSION Circulating tumor DNA is potentially a highly sensitive non-invasive biomarker among adults with gliomas. To maximize its sensitivity, CSF should be studied with targeted genetic analysis platforms, particularly in suspected high-grade gliomas.

A Systematic Review and Meta-analysis of the Association Between Circulating Tumor DNA (ctDNA) and Prognosis in Pancreatic Cancer

2021 ◽  
pp. 103528
Author(s):  
Deniz Can Guven ◽  
Taha Koray Sahin ◽  
Hasan Cagri Yildirim ◽  
Oktay Halit Aktepe ◽  
Omer Dizdar ◽  
...  
Keyword(s):  
Systematic Review ◽  
Pancreatic Cancer ◽  
Meta Analysis ◽  
Circulating Tumor Dna ◽  
Tumor Dna

Influence of Fatty Infiltration on Hip Abductor Repair Outcomes: A Systematic Review and Meta-analysis

2021 ◽  
pp. 036354652110279
Author(s):  
Austin M. Looney ◽  
Blake M. Bodendorfer ◽  
Stiles T. Donaldson ◽  
Robert B. Browning ◽  
Jorge A. Chahla ◽  
...  
Keyword(s):  
Systematic Review ◽  
Surgical Intervention ◽  
Meta Analysis ◽  
Fatty Infiltration ◽  
Harris Hip Score ◽  
Low Grade ◽  
High Grade ◽  
Vas Score ◽  
Hip Abductor ◽  
The Relationship

Background: Increasing evidence supports surgical intervention for hip abductor tears; however, the influence of fatty infiltration (FI) on outcomes after repair remains uncertain and has been addressed only in small case series. Purpose: To clarify the relationship between FI and surgical outcomes for hip abductor tears. Study Design: Meta-analysis; Level of evidence, 4. Methods: A systematic review and meta-analysis was conducted according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. FI severity was assessed by Goutallier-Fuchs (G-F) grade. The relationship between FI and improvement in Harris Hip Score (HHS)/modified Harris Hip Score (mHHS) and visual analog scale (VAS) score for pain was examined with mixed-effects metaregression. Outcomes with open and endoscopic techniques were also compared. Results: A total of 4 studies (206 repairs in 201 patients) were eligible. High-grade FI was associated with significantly less improvement in HHS/mHHS than both no FI (6.761 less; 95% CI, 3.983-11.570; P = .002) and low-grade FI (7.776 less; 95% CI, 2.460-11.062; P < .001) but did not significantly influence VAS score ( P > .05). Controlling for FI severity, we found no significant difference in HHS/mHHS improvement between open versus endoscopic repair ( P > .05 at each level), but open repair resulted in significantly greater improvement in VAS score for every G-F grade (all P < .005). Conclusion: Surgical intervention for symptomatic hip abductor tendon tears improved outcomes as reflected by change in HHS/mHHS; however, the presence of high-grade FI resulted in less improvement. FI severity did not influence VAS scores for pain. Although no differences were found between open and endoscopic repairs in terms of FI-adjusted improvement in HHS/mHHS, open repairs resulted in significantly greater pain relief at each FI level.

scholarly journals The diagnostic accuracy of circulating tumor DNA for the detection of EGFR-T790M mutation in NSCLC: a systematic review and meta-analysis

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Francesco Passiglia ◽  
Sergio Rizzo ◽  
Massimo Di Maio ◽  
Antonio Galvano ◽  
Giuseppe Badalamenti ◽  
...  
Keyword(s):  
Systematic Review ◽  
Diagnostic Accuracy ◽  
Meta Analysis ◽  
Circulating Tumor Dna ◽  
T790m Mutation ◽  
Tumor Dna ◽  
Egfr T790m

scholarly journals Role of diffusional kurtosis imaging in grading of brain gliomas: a protocol for systematic review and meta-analysis

BMJ Open ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. e025123 ◽  
Author(s):  
Gehad Abdalla ◽  
Eser Sanverdi ◽  
Pedro M Machado ◽  
Joey S W Kwong ◽  
Jasmina Panovska-Griffiths ◽  
...  
Keyword(s):  
Systematic Review ◽  
Quality Assessment ◽  
Brain Tumours ◽  
Meta Analysis ◽  
Sensitivity Analyses ◽  
Histopathological Analysis ◽  
Low Grade ◽  
Diffusional Kurtosis Imaging ◽  
Non Invasive ◽  
Statistical Heterogeneity

IntroductionCentral nervous system (CNS) gliomas are the most common primary intra-axial brain tumours and pose variable treatment response according to their grade, therefore, precise staging is mandatory. Histopathological analysis of surgical tumour samples is still deemed as the state-of-the-art staging technique for gliomas due to the moderate specificity of the available non-invasive imaging modalities. A recently evolved analysis of the tissue water diffusion properties, known as diffusional kurtosis imaging (DKI), is a dimensionless metric, which quantifies water molecules’ degree of non-Gaussian diffusion, hence reflects tissue microenvironment’s complexity by means of non-invasive diffusion-weighted MRI acquisitions. The objective of this systematic review and meta-analysis is to explore the performance of DKI in the presurgical grading of gliomas, both regarding the differentiation between high-grade and low-grade gliomas as well as the discrimination between gliomas and other intra-axial brain tumours.Methods and analysisWe will search PubMed, Medline via Ovid, Embase and Scopus in July 2018 for research studies published between January 1990 and June 2018 with no language restrictions, which have reported on the performance of DKI in diagnosing CNS gliomas. Robust inclusion/exclusion criteria will be applied for selection of eligible articles. Two authors will separately perform quality assessment according to the quality assessment of diagnostic accuracy studies-2 tool. Data will be extracted in a predesigned spreadsheet. A meta-analysis will be held using a random-effects model if substantial statistical heterogeneity is expected. The heterogeneity of studies will be evaluated, and sensitivity analyses will be conducted according to individual study quality.Ethics and disseminationThis work will be based on published studies; hence, it does not require institutional review board approval or ethics clearance. The results will be published in peer-reviewed journals.PROSPERO registration numberCRD42018099192.

Sign in / Sign up

Export Citation Format

Share Document