scholarly journals Clinical Significance of Staphylococcus aureus in a Single Positive Blood Culture Bottle

2021 ◽  
Author(s):  
John Raymond Go ◽  
Douglas Challener ◽  
Cristina Corsini Campioli ◽  
M Rizwan Sohail ◽  
Raj Palraj ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Infective Endocarditis ◽  
Blood Culture ◽  
Clinical Significance ◽  
Positive Blood Culture ◽  
Blood Culture Bottle ◽  
Hospital Length Of Stay ◽  
Culture Bottle ◽  
Single Positive ◽  
Clinically Significant

Abstract Clinical significance of a single positive blood culture bottle (SPBCB) with Staphylococcus aureus is unclear. We aimed to assess the significance of a SPBCB by looking at the associated outcomes. We performed a retrospective, multicenter study of patients with a SPBCB with S. aureus using data collected from both electronic health records and the clinical microbiology laboratory. Overall, 534 patients with S. aureus bacteremia were identified and 118 (22.1%) had a SPBCB. Among cases with a SPBCB, 106 (89.8%) were classified as clinically significant while 12 (10.2%) were considered contaminated or of unclear significance. A majority (92.4%) of patients received antibiotic therapy but patients with clinically significant bacteremia were treated with longer courses (25.9 vs 5.7 days, P<0.001). Significant differences in both frequency of echocardiography (65.1% vs 84.6%, P<0.001), and infective endocarditis diagnosis (3.8% vs 14.2%, P=0.002) were seen in those with a single positive blood culture bottle compared to those with multiple positive bottles. A longer hospital length of stay, and higher 90-day, 6-month, and 1-year mortality rates were seen in patients with multiple positive blood culture bottles. A SPBCB with S. aureus was common among our patients. While this syndrome has a more favorable prognosis as compared to those with multiple positive blood cultures, clinicians should remain concerned as it portends a risk of infective endocarditis and mortality.

scholarly journals 6. Staphylococcus aureus in a Single Blood Culture Bottle: Should We be Concerned?

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S5-S6
Author(s):  
John Raymond U Go ◽  
Douglas Challener ◽  
Cristina G Corsini Campioli ◽  
Muhammad R Sohail ◽  
Raj Palraj ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Clinical Significance ◽  
Positive Blood Culture ◽  
Laboratory Finding ◽  
Clinical Importance ◽  
Blood Culture Bottle ◽  
Clinical Microbiology Laboratory ◽  
Culture Bottle ◽  
Clinically Significant

Abstract Background Staphylococcus aureus bacteremia (SAB) is common and is characterized by high rates of morbidity and mortality. The clinical importance of a single positive blood culture bottle (SPBCB), however, is poorly defined despite it being a frequent laboratory finding. We therefore examined patients with SPBCB to determine its clinical significance and to understand the rationale of current practice. Methods We performed a retrospective, multicenter study of patients with a SPBCB for S. aureus in initial cultures from January 2019 to December 2019 using data collected from both electronic health records and the clinical microbiology laboratory. Results Overall, 534 patients with SAB were identified, and 118 (22.1%) had a SPBCB. Among SPBCB cases, 106 (89.3%) were classified as clinically significant while 12 were considered contaminated or of unclear clinical significance. Baseline characteristics were similar between the groups (Table 1). A majority (92.4%) received antibiotic therapy, but patients with clinically significant bacteremia were treated with a longer antibiotic course (25.9 vs 5.7 days, p< 0.001). Outcomes between those with SPBCB (contaminant vs clinically significant) were similar (Table 2). Of note, while there was no difference in use of echocardiography based on PREDICT criteria between the clinically significant SPBCB vs. the multiple positive blood culture bottles (MPBC) cohorts (Table 3), significant differences were seen in both frequency of echocardiography (65.1% vs. 84.6%, P< 0.001) and IE diagnosis (3.8% vs. 14.2%, P=0.002) for patients in the SPBCB vs. MPBC groups, respectively. In addition, those with MPBC had higher 90-day, 6-month and 1-year mortality rates. Conclusion SPBCB was documented in almost one-quarter of SAB cases and should trigger a thorough investigation as its associated mortality was high and complications, including IE, occurred. Although some SPBCB cases may represent contamination, antibiotic treatment of SPBCB was commonplace. Patients with clinically significant SPBCB were less likely to undergo echocardiography and had a reduced prevalence of an IE diagnosis as compared to those with MPBC. Patients with SPBCB may have a more favorable long-term prognosis as compared to that in patients with MPBC. Disclosures Muhammad R. Sohail, MD, Medtronic (Consultant)Philips (Consultant) Larry M. Baddour, MD, Boston Scientific (Individual(s) Involved: Self): Consultant; Botanix Pharmaceuticals (Individual(s) Involved: Self): Consultant; Roivant Sciences (Individual(s) Involved: Self): Consultant

Nosocomial Bacteremia Clinical Significance of a Single Blood Culture Positive for Coagulase-Negative Staphylococci

2005 ◽  
Vol 26 (8) ◽  
pp. 697-702 ◽  
Author(s):  
Benoît Favre ◽  
Stéphane Hugonnet ◽  
Luci Correa ◽  
Hugo Sax ◽  
Peter Rohner ◽  
...  
Keyword(s):  
Blood Culture ◽  
Clinical Significance ◽  
Positive Blood Culture ◽  
Blood Cultures ◽  
University Teaching ◽  
Coagulase Negative Staphylococci ◽  
Nosocomial Bacteremia ◽  
Single Positive ◽  
Clinically Significant ◽  
Culture Positive

AbstractObjectives:To describe the epidemiology of nosocomial coagulase-negative staphylococci (CoNS) bacteremia and to evaluate the clinical significance of a single blood culture positive for CoNS.Design:A 3-year retrospective cohort study based on data prospectively collected through hospital-wide surveillance. Bacteremia was defined according to CDC criteria, except that a single blood culture growing CoNS was not systematically considered as a contaminant. All clinically significant blood cultures positive for CoNS nosocomial bacteremia were considered for analysis.Setting:A large university teaching hospital in Geneva, Switzerland.Results:A total of 2,660 positive blood cultures were identified. Of these, 1,108 (41.7%) were nosocomial; CoNS were recovered from 411 nosocomial episodes (37.1%). Two hundred thirty-four episodes of CoNS bacteremia in the presence of signs of sepsis were considered clinically relevant and analyzed. Crude mortality and associated mortality were 24.4% and 12.8%, respectively. Associated mortality was similar among patients with one positive blood culture and those with two or more (16.2% vs 10.8%, respectively;P= .3). Mortality rates after bacteremia for patients with a single positive blood culture and for those with two or more were 15.3% and 7.0%, respectively, at day 14 (RR, 2.2; CI%, 0.87-5.46) and 20.8% and 11.3%, respectively, at day 28 (RR, 1.9; CI95, 0.9-3.8). On multivariate analysis, only age and a rapidly fatal disease were independently associated with death.Conclusion:CoNS bacteremia harbor a significant mortality and a single positive blood culture in the presence of signs of sepsis should be considered as clinically relevant.

Clinical significance of follow-up blood culture in patients with a single Staphylococcus aureus-positive blood culture

2019 ◽  
Vol 52 (3) ◽  
pp. 207-212
Author(s):  
Taeeun Kim ◽  
Seung Cheol Lee ◽  
Min Jae Kim ◽  
Jiwon Jung ◽  
Heungsup Sung ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Clinical Significance ◽  
Positive Blood Culture

Two Epidemics of Pseudobacteremia Due to Staphylococcus aureus and Aerococcus viridans

1980 ◽  
Vol 1 (5) ◽  
pp. 321-323 ◽  
Author(s):  
Martin L. Spivack ◽  
Robert Shannon ◽  
George A. Natsios ◽  
Jan Wood
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Culture System ◽  
Skin Infection ◽  
Blood Culture Bottle ◽  
Nasal Colonization ◽  
Culture Bottle ◽  
Blood Culture System ◽  
Aerococcus Viridans ◽  
Staphyloccus Aureus

AbstractTwo epidemics of pseudobacteremia are reported. The first, due to Staphyloccus aureus, was caused by a physician who had active staphylococcal skin infection and nasal colonization. Because the blood culture system in use at the time was open, and used screw cap bottles, we assume that the physician contaminated the bottles at the time of inoculation. The second outbreak, caused by Aerococcus viridans, was traced to contamination of the blood culture bottle tops as they were received from the manufacturer. We assume that there was inadequate disinfection of the bottle tops by the physicians prior to their use.

Superiority of SDS lysis over saponin lysis for direct bacterial identification from positive blood culture bottle by MALDI-TOF MS

2016 ◽  
Vol 11 (5-6) ◽  
pp. 1600131 ◽  
Author(s):  
Yvan Caspar ◽  
Cécile Garnaud ◽  
Mariya Raykova ◽  
Sébastien Bailly ◽  
Marie Bidart ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Blood Culture Bottle ◽  
Bacterial Identification ◽  
Culture Bottle ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Bacteraemia due to meticillin-resistant Staphylococcus aureus carrying the mecC gene in a patient with urothelial carcinoma

2013 ◽  
Vol 62 (12) ◽  
pp. 1914-1916 ◽  
Author(s):  
María Pilar Romero-Gómez ◽  
Marta Mora-Rillo ◽  
Fernando Lázaro-Perona ◽  
María Rosa Gómez-Gil ◽  
Jesús Mingorance
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Blood Culture Bottle ◽  
Culture Bottle ◽  
Negative Results ◽  
Specific Pcr ◽  
Conventional Culture ◽  
Vitek 2

We present a case of bacteraemia due to meticillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene. The susceptibility to meticillin of Staphylococcus aureus was investigated directly from one blood culture bottle using GenomEra MRSA/SA (Abacus Diagnostica Oy) test. This test identified S. aureus but the presence of the mecA gene result was negative, and the isolate was reported as meticillin-sensitive Staphylococcus aureus (MSSA). Susceptibility studies were done using VITEK 2 AST-P588 susceptibility cards (bioMérieux). The strain was identified as MRSA by the VITEK 2 system, although oxacillin MIC was low (0.5 µg ml−1). In view of these results, the isolate was tested for the presence of the mecC gene by a specific PCR and was verified as MRSA carrying mecC. The emergence of this new mecA homologue could have important consequences for the detection of MRSA when routine PCR methods are used as an identification method or provisional detection of MRSA, as in the case reported in this article, because S. aureus carrying the mecC gene will be wrongly diagnosed as meticillin susceptible. Negative results must be interpreted with caution and should be followed by conventional culture, and antimicrobial susceptibility testing or detection of mecC gene by a specific PCR.

Mobile genetic elements responsible for discordant Staphylococcus aureus phenotypes and genotypes in the same blood culture bottle

2020 ◽  
Vol 98 (4) ◽  
pp. 115175
Author(s):  
Isabella A. Tickler ◽  
Richard V. Goering ◽  
Scott Dewell ◽  
Victoria M. Le ◽  
Leepakshi Johar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Mobile Genetic Elements ◽  
Blood Culture Bottle ◽  
Culture Bottle ◽  
Genetic Elements
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