scholarly journals In Vitro Activity of Essential Oils Against Gram-Positive and Gram-Negative Clinical Isolates, Including Carbapenem-Resistant Enterobacteriaceae

2019 ◽  
Vol 6 (12) ◽  
Author(s):  
Jan E Patterson ◽  
Leticia McElmeel ◽  
Nathan P Wiederhold
Keyword(s):  
Essential Oils ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Gram Positive ◽  
Cinnamon Bark ◽  
Carbapenem Resistant ◽  
Zones Of Inhibition ◽  
Carbapenem Resistant Enterobacteriaceae

Abstract Background There is increasing demand for compounds to treat antimicrobial-resistant pathogens, and essential oils have gained interest. Moreover, previous studies have demonstrated antimicrobial activity of these nonpharmaceutical products. We investigated the activity of essential oils against multiresistant bacteria and other clinical isolates to evaluate the potential of their use topically and/or internally for treatment of bacterial infections. Methods We studied the in vitro activity of 10 essential oils and 1 essential oil blend against clinical isolates including extended-spectrum beta-lactamase-producing Enterobacteriaceae, carbapenem-resistant Enterobacteriaceae, multidrug-resistant Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus. Results Essential oils of oregano, thyme, cinnamon bark, and lemongrass had the largest zones of inhibition against Gram-positive organisms, whereas cinnamon bark had the largest zone of inhibition against P aeruginosa. Oregano, thyme, and cinnamon bark had the largest zones of inhibition against Enterobacteriaceae. Conclusions Essential oils have promising in vitro activity that warrants further study of their activity and use in the clinical setting.

scholarly journals In vitro Susceptibility Testing of Essential Oils against Gram-positive and Gram-negative Clinical Isolates, including Carbapenem-resistant Enterobacteriaceae (CRE)

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S370-S370
Author(s):  
Jan E Patterson ◽  
M.L McElmeel ◽  
Nathan P Wiederhold
Keyword(s):  
Essential Oils ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
Resistant Bacteria ◽  
In Vitro Activity ◽  
Gram Positive ◽  
Gram Negative ◽  
Cinnamon Bark ◽  
Zones Of Inhibition

Abstract Background In the era of antibiotic resistance, alternative anti-infectives must be explored. The National Action Plan for Combating Antibiotic-Resistant Bacteria calls for developing nontraditional therapeutics, including natural compounds such as essential oils (EOs) (Goal 4.4). A pilot study previously showed in vitro activity of EOs against CRE and warranted further study of their antibacterial activity. We studied cinnamon bark, clove, lavender, lemongrass, eucalyptus, oregano, rosemary, thyme, tea tree, manuka, and Thieves® blend (Young Living Essential Oils, Lehi UT) against an expanded panel of Gram-positive and Gram-negative isolates. Methods 30 Gram-positive and 70 Gram-negative clinical isolates, including CRE, were tested using CLSI methods. Isolates were grown overnight on TSA; 0.5 McFarland suspensions in sterile water were swabbed over Mueller–Hinton agar using the Kirby–Bauer method. 20 μl of full-strength oils were pipetted onto blank paper disks in a sterile dish. Disks were placed aseptically onto the plates immediately after inoculating disks. Vancomycin was tested with Gram-positives and meropenem with Gram-negatives. Median zone diameters are shown. Results EOs oregano, thyme, cinnamon bark, and lemongrass had the largest zones of inhibition against Gram-positive organisms and were larger than those of vancomycin for MRSA/MSSA. Cinnamon bark had the largest zone of inhibition against P. aeruginosa and was larger than that of meropenem. Oregano, thyme, cinnamon bark had the largest zones of inhibition against Enterobacteriaceae and were larger than those of meropenem against K. pneumoniae and E. cloacae. Conclusion Essential oils showed significant in vitro activity against clinical isolates, including CRE. Further study of the clinical activity of essential oils is warranted. Disclosures J. E. Patterson, Young Living Essential Oils: Independent Contractor, Salary

scholarly journals In vitro activity of tigecycline against 313 Gram-positive and Gram-negative clinical isolates

2010 ◽  
Vol 25 (1) ◽  
Author(s):  
Elisabetta Maioli ◽  
Erika Coppo ◽  
Ramona Barbieri ◽  
Elisabetta Canepa ◽  
Laura Gualco ◽  
...  
Keyword(s):  
Vitro Activity ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Gram Positive ◽  
Gram Negative

scholarly journals 681. In vitro Activity of the β-Lactamase Inhibitor QPX7728 in Combination with Several β-Lactams Against Acinetobacter baumannii (AB) and Pseudomonas aeruginosa (PSA)

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S310-S311 ◽  
Author(s):  
Olga Lomovskaya ◽  
Jill Lindley ◽  
Debora Rubio-Aparicio ◽  
Kirk J Nelson ◽  
Mariana Castanheira
Keyword(s):  
Resistance Mechanisms ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Microdilution Method ◽  
Carbapenem Resistant ◽  
Broth Microdilution Method ◽  
Data Representative ◽  
Lactamase Inhibitor

Abstract Background QPX7728 (QPX) is a novel broad-spectrum boron-containing inhibitor of serine- and metallo-β-lactamases (MBLs). We evaluated the in vitro activity of QPX combined with several β-lactams against carbapenem-resistant AB (CRAB) and PSA clinical isolates with varying β-lactam resistance mechanisms. Methods A total of 503 CRAB (meropenem [MEM] MIC ≥8 µg/mL) and 762 PSA clinical isolates were tested by the reference broth microdilution method against β-lactams alone and combined with QPX (4 µg/mL and 8 µg/mL). PSA isolates were selected to represent the normal distribution of MEM, ceftazidime–avibactam (CAZ-AVI), and ceftolozane-tazobactam (TOL-TAZ) resistance according to 2017 surveillance data (representative panel). Additionally, 262 PSA isolates that were either nonsusceptible (NS) to MEM (MIC, ≥4 µg/mL) or to TOL-TAZ (MIC, ≥8 µg/mL), or resistant (R) to CAZ-AVI (MIC, ≥16 µg/mL) (challenge panel) were also tested. Within this 262 strain challenge set, 56 strains carried MBLs and the majority also had nonfunctional OprD. Results Against CRAB, QPX at 4 and 8 µg/mL increased the potency of all β-lactams tested. MEM-QPX was the most potent combination (table) displaying MIC50/MIC90 at 1/8 and 0.5/4 µg/mL with QPX at fixed 4 and 8 µg/mL, respectively. Susceptibility (S) to MEM was restored in >95% of strains. Against the 500 PSA from the representative panel, S for all QPX combinations was >90%. For the challenge panel, TOL-QPX and piperacillin (PIP)-QPX were the most potent combinations, restoring S in 76–77% of strains. TOL-QPX and MEM-QPX or cefepime (FEP)-QPX restored the MIC values to S rates when applying the CLSI breakpoint for the compound alone (comparison purposes only) in ~90% and ~75% of non-MBL-producing strains, respectively, vs. 60–70% for TOL-TAZ and CAZ-AVI. PIP-QPX reduce the MIC values to S values for PIP-TAZ in ~60% of MBL-producing strains vs. 20–30% and 3–7% for other QPX combinations and non-QPX tested combinations, respectively. Conclusion Combinations of QPX with various β-lactam antibiotics displayed potent activity against CRAB and resistant PSA isolates and warrant further investigation. Disclosures All authors: No reported disclosures.

In vitro Activity of Quinupristin/ Dalfopristin, RP59500, against Gram-Positive Clinical Isolates

Chemotherapy ◽  
1997 ◽  
Vol 43 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Hussain Qadri ◽  
Y. Ueno ◽  
Abu Mostafa ◽  
Majid Halim
Keyword(s):  
Vitro Activity ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Gram Positive

scholarly journals In vitro activity of telavancin against recent Gram-positive clinical isolates: results of the 2004-05 Prospective European Surveillance Initiative

2008 ◽  
Vol 62 (1) ◽  
pp. 116-121 ◽  
Author(s):  
D. C. Draghi ◽  
B. M. Benton ◽  
K. M. Krause ◽  
C. Thornsberry ◽  
C. Pillar ◽  
...  
Keyword(s):  
Vitro Activity ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Gram Positive

In vitro Activity of Biapenem against Recent Gram-Negative and Gram-Positive Clinical Isolates

Chemotherapy ◽  
1997 ◽  
Vol 43 (6) ◽  
pp. 393-399 ◽  
Author(s):  
Giovanni Bonfiglio ◽  
Giuseppa Maccarone ◽  
Maria Lina Mezzatesta ◽  
Angela Privitera ◽  
Vincenzo Carciotto ◽  
...  
Keyword(s):  
Vitro Activity ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Gram Positive ◽  
Gram Negative

In vitro activity of quinupristin/dalfopristin against clinical isolates of common gram-positive bacteria in Taiwan

1999 ◽  
Vol 33 (4) ◽  
pp. 299-303 ◽  
Author(s):  
Shan-Chwen Chang ◽  
Chi-Tai Fang ◽  
Po-Ren Hsueh ◽  
Kwen-Tay Luh ◽  
Wei-Chuan Hsieh
Keyword(s):  
Vitro Activity ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Gram Positive ◽  
Gram Positive Bacteria
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