Acrosin activity in human spermatozoa in relation to semen quality and in-vitro fertilization

1993 ◽  
Vol 8 (2) ◽  
pp. 253-257 ◽  
Author(s):  
C.J. De Jonge ◽  
S.M. Tarchala ◽  
R.G. Rawlins ◽  
Z. Binor ◽  
E. Radwanska
Keyword(s):  
In Vitro Fertilization ◽  
Semen Quality ◽  
Human Spermatozoa ◽  
Vitro Fertilization ◽  
Acrosin Activity

scholarly journals IN VITRO FERTILITY TEST OF HUMAN SPERMATOZOA MEMBRANE PROTEIN FERTILIN BETA ANTIBODY IN MICE (Mus musculus Balb/c) AS IMMUNOCONTRACEPTIVE CANDIDATE

2017 ◽  
Vol 52 (3) ◽  
pp. 209
Author(s):  
Reny I’tishom ◽  
Doddy M Soebadi ◽  
Aucky Hinting ◽  
Hamdani Lunardhi ◽  
Rina Yudiwati
Keyword(s):  
In Vitro Fertilization ◽  
Membrane Protein ◽  
Mus Musculus ◽  
Human Spermatozoa ◽  
Control Group ◽  
Reproductive Process ◽  
Vitro Fertilization ◽  
Antibody Induction

One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.

Association of state and trait anxiety to semen quality of in vitro fertilization patients: a controlled study

2013 ◽  
Vol 99 (6) ◽  
pp. 1565-1572.e2 ◽  
Author(s):  
Elisa Vellani ◽  
Alessandro Colasante ◽  
Luciana Mamazza ◽  
Maria Giulia Minasi ◽  
Ermanno Greco ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Trait Anxiety ◽  
Semen Quality ◽  
Controlled Study ◽  
Vitro Fertilization ◽  
State And Trait Anxiety

Stress and Semen Quality in an In Vitro Fertilization Program

1988 ◽  
Vol 139 (5) ◽  
pp. 1138-1139 ◽  
Author(s):  
K.L. Harrison ◽  
V.J. Callan ◽  
J.F. Hennessey
Keyword(s):  
In Vitro Fertilization ◽  
Semen Quality ◽  
Fertilization Program ◽  
Vitro Fertilization

T08-O-20 Relationship between anxiety, psychological stress and semen quality among in vitro fertilization patients

Sexologies ◽  
2008 ◽  
Vol 17 ◽  
pp. S111
Author(s):  
E. Vellani ◽  
A. Colasante ◽  
D. Zavaglia ◽  
F. Di Nocera ◽  
A. Bevilacqua ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Psychological Stress ◽  
Semen Quality ◽  
Vitro Fertilization

Correlation between Progesterone Binding Sites on Human Spermatozoa and in vitro Fertilization Outcome

2000 ◽  
Vol 49 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Keisuke Fukui ◽  
Reiko Hori ◽  
Isao Yoshimoto ◽  
Hiroshi Ochi ◽  
Masaharu Ito
Keyword(s):  
In Vitro Fertilization ◽  
Binding Sites ◽  
Human Spermatozoa ◽  
Vitro Fertilization

scholarly journals The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity

2020 ◽  
Vol 9 (8) ◽  
pp. 2327
Author(s):  
Pernille Badsberg Norup ◽  
Dorte L. Egeberg Palme ◽  
Morten R. Petersen ◽  
Katharina M. Main ◽  
Kristian Almstrup
Keyword(s):  
In Vitro Fertilization ◽  
Semen Quality ◽  
Area Under The Curve ◽  
Image Cytometry ◽  
Vitro Fertilization ◽  
Sperm Donors ◽  
Fertility Treatments ◽  
Significant Difference ◽  
Fertility Clinic

The acrosome of the spermatozoa is required for fertilization and in the raw ejaculate the percentage of viable acrosome-intact spermatozoa, the acrosomal status, is higher among men with good semen quality. Here we investigated if the acrosomal status of the processed semen preparations used at a fertility clinic can also be informative and whether it is associated with fecundity. The acrosomal status was measured by image cytometry on purified semen samples from couples during in vitro fertilization (IVF) (n = 99) and intracytoplasmic sperm injection (ICSI) (n = 107) treatment. Purified frozen-thawed donor samples were also analyzed (n = 199). In purified semen preparations the acrosomal status was significantly higher among sperm donors (p = 5.3 × 10−8) and men from IVF couples (p = 2.2 × 10−5) when compared to men from ICSI couples. A significant difference was also found between female, male and mixed factor infertility (p = 0.003). No association with lifestyle factors was found. In frozen-thawed donor samples, a significant positive (r = 0.16, p = 0.025) association with the number of pregnancies per sold straw was observed together with an area under the curve of 75.3%, when comparing the top and bottom deciles. Our results indicate that the acrosomal status may be a valuable parameter for personalizing fertility treatments and might be a good predictor of pregnancy success among normozoospermic men.

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